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EC number: 200-240-8 | CAS number: 55-63-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- November 1987-March 1991
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets National standards method with acceptable resctrictions.
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM Designation E 729-80 (ASTM, 1980)
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Details on sampling:
- - The stability of the NG stock solution were periodically checked during storage to assure that no decomposition occured. Standard solutions of NG were prepared by dilution of the stock solution freshly prepared each day of analysis. Standard solutions with concentrations of 10.0, 5.0, 2.5, and 1.0 µl/L were used.- Aqueous samples from bioassays were injected directly into HPLC after filtration to remove particles > 0,45 µm.In the cases where samples could not be analysed immediately following filtration, the filtered samples were stored at 4°C in amber glass vials fitted with Teflon-lined caps and analyzedwithin 24 h from the time the samples were originally taken from the test aquaria.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION - Basic solution (the shipment): 1 g NG dissolved in JHU/APL well water. NG solutions were keep in the dark at 4°C in glass bottles.- NG stock solutions were prepared by adding appropriate amounts of the material to aerated JHU/APL well water and stirred for 4 to 8 h. No NG stock solutions were heated during preparation or filtering at 0.45 µm. All stock solutions were prepared in amber glass containers.
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM- Common name: Fathead minnow- Source: the JHU/APL culture. The JHU/APL culture was initiated with mature fathead minnows obtained from the U.S.EPA Environmental Monitoring and Support Laboratory-Cincinnati, Ohio.- Age at study initiation (mean and range, SD): juvenile (20 days)- Mean length (control organisms) at study end: 10.7 +/- 1.9 mm(n=10)- Mean dry weight (control organisms) at study end: 3.8 +/- 2.5 mg (n=10)- Method of breeding: Spawning fish were cultured in fiber glass tanks containing JHU/APL well water held at 25 +/- 1 °C. The spawning adults were fed a diet of frozen brine shrimp (Artemia sp.) and TetraMin® staple food. twice daily.Excess food was removed daily. Five sets of spawning fathead minnows were maintained in the culture tanks at ration of 1 male : 3 females. Replacement spawners were rotated at approx. 3-month intervals. Fathead minnow embryos were collected on spawning substrates. Fry were reared on brine shrimp nauplii in 19 L aquaria at 25 +/- 1°C in JHU/APL well water.ACCLIMATION- Since they had been bred and raised under the test conditions, no acclimatization was necessary.
- Test type:
- flow-through
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Hardness:
- 199 (120-240) mg/L as CaCO3
- Test temperature:
- 25.3 (24.7-25.7)°C
- pH:
- 7.4 (7-7.9)
- Dissolved oxygen:
- 7.4 (7-7.9) mg/L
- Nominal and measured concentrations:
- Nominal concentration: 1.04, 1.76, 3.68, 4.8, 8 mg/LMeasured concentartion (mean): 0.94, 1.63, 3.53, 4.51, 7.71 mg/L
- Details on test conditions:
- TEST SYSTEM- Test vessel: 10-L glass aquarium- Fill volume: 6.4 L- Volume additions per day: 4.8 L (mean volume addition per day for the complete exposure period)- Type of flow-through: solenoid-activated proportional dilutor systems- No. of organisms per vessel: 10- No. of vessels per concentration (replicates): 2- No. of vessels per control (replicates): 2- Biomass loading rate: 0.03 g/L (mean loading (g wet weight/L) at the end of the exposure period for control organisms)TEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: Non-chlorinated deep well located at The Johns Hopkins University Applied Physics Laboratory (JHU/APL) in Shady Side, MD.- Total organic carbon: 19 mg/L- Metals: Sb, As, Be, Pb, se, Tl < 0.005 mg/L, Cd < 0.001 mg/L, Cr < 0.05 mg/L, Cu < 0.02 mg/L, Hg < 0.0002 mg/L, Ni < 0.2 mg/L, Ag <0.01 mg/L, Zn < 0.04 mg/L- Alkalinity:100 mg CaCO3/L- Conductivity: 345 µmhos/cm- The water used in was analyzed for 43 Priority Pollutants, eight nonpriority, but "Hazardous" substances, 26 pesticides, and 13 metals. None of them were detected with detection limits of 2, 2, 0.1 µg/, and <0.2 - <0.0002 mg/, respectively.- Intervals of water quality measurement: A comprehensive chemical analysis of the well water was conducted two times during the study. The analyses were separated by approx. 18 months.OTHER TEST CONDITIONS- Photoperiod:16-h light:8-h dark- Light intensity: fluorescent lights, 60-85 foot candles at the surface of test chambersEFFECT PARAMETERS MEASURED: mortality at 24, 48, 72 and 96 hrsTEST CONCENTRATIONS- Concentrations for the determinative evaluations were set with two objectives: 1) a geometric series of five concentrations with a dilution factor of 0.6 and 2) two of the five concentrations to produce approximately 84% survival and 84% death, respectively.
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3.58 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 2.91-4.41 mg/L
- Reported statistics and error estimates:
- The LC50 values and their 95% fiducial limits were determined by the probit method, using an EPA statistical program (Stephan, 1978). In all cases, thegoodness of fit probability of the data was >0.05.
- Conclusions:
- The 96 hr. LC50 for fingerling fathead minnows in this flow-through study was 3.58 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- November 1987-March 1991
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets National standards method with acceptable resctrictions.
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM Designation E 729-80 (ASTM, 1980)
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Details on sampling:
- - The stability of the NG stock solution were periodically checked during storage to assure that no decomposition occured. Standard solutions of NG were prepared by dilution of the stock solution freshly prepared each day of analysis. Standard solutions with concentrations of 10.0, 5.0, 2.5, and 1.0 µl/L were used.- Aqueous samples from bioassays were injected directly into HPLC after filtration to remove particles > 0,45 µm.In the cases where samples could not be analysed immediately following filtration, the filtered samples were stored at 4°C in amber glass vials fitted with Teflon-lined caps and analyzedwithin 24 h from the time the samples were originally taken from the test aquaria.
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION - Basic solution (the shipment): 1 g NG dissolved in 1 L of ethanol. NG solutions were keep in the dark at 4°C in glass bottles.- NG stock solutions were prepared by adding appropriate amounts of the material to aerated JHU/APL well water and stirred for 4 to 8 h. No NG stock solutions were heated during preparation or filtering at 0.45 µm. All stock solutions were prepared in amber glass containers.- Chemical name of vehicle: ethanol
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM- Common name: Rainbow trout- Source: Pennsylvania Fish Commission, Reynoldsdale Fish Culture Station, New Paris, PA- Age at study initiation: fingerling (48-49 d)- Mean length (control organisms) at study end: 29.5 +/- 1.4 mm (mean size of control organisms)- Mean weight (control organisms) at study end: 314 +/- 50 mg (mean wet weight)ACCLIMATION- Acclimation period: 14-16 d prior to testing- Acclimation conditions: in aerated JHU/APL well water at 12 +/- 1°C- Type and amount of food:ground SalmonStarter Meal (Zielger Brothers, Gardners, PA)- Feeding frequency: three times dailyon weekdays and twice on weekends
- Test type:
- flow-through
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Hardness:
- (176-189) mg/L as CaCO3
- Test temperature:
- 11.5 (10.7-11.9) °C181
- pH:
- 8.3 (7.8-8.9)
- Dissolved oxygen:
- 9.7 (9-10.2) mg/L
- Nominal and measured concentrations:
- Nominal concentration: 1, 1.67, 2.78, 4.63, 7.72 mg/LMeasured concentration (mean): 0.91, 1.46, 2.47, 3.89, 6.25 mg/L
- Details on test conditions:
- TEST SYSTEM- Test vessel: 10-L glas aquarium- Fill volume: 6.4 LVolume additions per day: 6 L (mean volume addition per day for the complete exposure period)- Type of flow-through: solenoid-activated proportional dilutor systems- No. of organisms per vessel: 10- No. of vessels per concentration (replicates): 2- No. of vessels per control (replicates): 2- No. of vessels per vehicle control (replicates): 2- Biomass loading rate: 0.03 g/L (mean loading (g wet weight/L) at the end of the exposure period for control organisms)TEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: Non-chlorinated deep well located at The Johns Hopkins University Applied Physics Laboratory (JHU/APL) in Shady Side, MD.- Total organic carbon: 19 mg/L- Metals: Sb, As, Be, Pb, se, Tl < 0.005 mg/L, Cd < 0.001 mg/L, Cr < 0.05 mg/L, Cu < 0.02 mg/L, Hg < 0.0002 mg/L, Ni < 0.2 mg/L, Ag <0.01 mg/L, Zn < 0.04 mg/L- Alkalinity: 100 (80-130) mg CaCO3/L- Conductivity: 345 (300-390) µmhos/cm- The water used in was analyzed for 43 Priority Pollutants, eight nonpriority, but "Hazardous" substances, 26 pesticides, and 13 metals. None of them were detected with detection limits of 2, 2, 0.1 µg/, and <0.2 - <0.0002 mg/, respectively.- Intervals of water quality measurement: A comprehensive chemical analysis of the well water was conducted two times during the study. The analyses were separated by approx. 18 months.OTHER TEST CONDITIONS- Adjustment of pH:- Photoperiod: 16-h light:8-h dark- Light intensity: fluorescent lights, 60-85 foot candles at the surface of the test chambersEFFECT PARAMETERS MEASURED: mortality at 24, 48, 72 and 96 hrsTEST CONCENTRATIONS- Concentrations for the determinative evaluations were set with two objectives: 1) a geometric series of five concentrations with a dilution factor of 0.6 and 2) two of the five concentrations to produce approximately 84% survival and 84% death, respectively.
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.9 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 1.69-2.14 mg/L
- Reported statistics and error estimates:
- The LC50 values and their 95% fiducial limits were determined by the probit method, using an EPA statistical program (Stephan, 1978). In all cases, thegoodness of fit probability of the data was >0.05.
- Conclusions:
- The 96 hr. LC50 for fingerling rainbow trout in this flow-through study was 1.90 mg/L.
Referenceopen allclose all
Description of key information
The contact of the nitroglycerine with the marine water is impossible in normal conditions of its production and using of explosives.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 1.98 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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