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Diss Factsheets
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EC number: 443-510-2 | CAS number: 738587-10-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Study test phase 2002-10-28 to 2002-11-01. Study report 2002-12-03
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Meets the criteria for classification as Reliable without restriction according to Klimisch et al (1997)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 443-510-2
- EC Name:
- -
- Cas Number:
- 738587-10-5
- Molecular formula:
- C26H14N10Ni2O18S4 as free acid
- IUPAC Name:
- dinickel(2+) sodium 3-carboxy-5-[(1E)-2-(7-oxido-2,6-disulfonaphthalen-1-yl)diazen-1-yl]-1H-1,2,4-triazol-1-ide 3-carboxy-5-[(1E)-2-(7-oxido-2-sulfo-6-sulfonatonaphthalen-1-yl)diazen-1-yl]-1H-1,2,4-triazol-1-ide
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The study was run with nominal concentrations of 1500 mg/L of test material together with a dilution water control.
Test organisms
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: Mirror carp
- Source: Reputable supplier
- Length at end of study: 36-40mm, mean 38mm
- Weight at end of study: 1.21-2.57g, mean 1.87g
- Feeding during test: none
ACCLIMATION
- Acclimation period: 7d
- Acclimation conditions: 22 +/- 2 degrees C
- Type of food: commercial fish food (food was witheld at least 24h prior to commencement of test)
- Health during acclimation:No mortalities observed
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- Hardness of dilution water 41.7 mg/L CaCO3
- Test temperature:
- 21.8-22.2 degrees C
- pH:
- 7.7-8.3
- Dissolved oxygen:
- 89-94%
- Salinity:
- Salinity of dilution water 1.0
- Nominal and measured concentrations:
- The overall mean measured concentration was 107% of the nominal value. The percentage loss in the measured concentrations over the test period was <1%. On the basis of the analytical data the nominal concentrations were used for the calculation and reporting of results.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Borosilicate glass
- Fill volume: Vessels had a max. capacity of 40L and a working volume of 25L
- Aeration: Test solutions were gently aerated
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates):1
- No. of vessels per control (replicates):1
- Biomass loading rate: 0.75g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water which has been passed through activated carbon, coarsely filtered, dechlorinated, salts added (to maintain hardness), UV sterilised and re-filtered (5um).
- Chlorine (as Cl2): Free residual <2 ug/L Combined residual <2 ug/L
- Alkalinity: 20.4 mg/L (as CaCO3)
- Conductivity: 243 uS/cm (at 25 degrees C)
- Intervals of water quality measurement: Daily measurements of the test solutions for pH, temperaure and dissolved oxygen. The temperature of the dilution water control was measured hourly.
OTHER TEST CONDITIONS
- Photoperiod: 16 hours fluorescent light and 8 hours dark with a 20 min. dawn/dusk transition period.
EFFECT PARAMETERS MEASURED: Observation for mortalities were made at 3, 24, 48, 72 and 96 h.
TEST CONCENTRATIONS
- Test concentrations: 1500 mg/L - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 500 mg/L
- Details on results:
- Due to the intensity of colour in the test solution it was not possible to assess symptoms of toxicity during the study.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- No mortalities were observed in either the control or the 1500 mg/L test concentration vessel during this study. Therefore, the 96h LC50 was determined to be >1500 mg/L. The No Observed Effect Concentration (NOEC) was determined to be 1500 mg/L. The lowest nominal concentration at which there was 100% mortality was >1500 mg/L.
- Executive summary:
Introduction
The study was undertaken to determine the acute toxicity of the test substance to mirror carp Cyprinus carpio. The method was designed to be compatible with the following guidelines:
OECD Guidelines for the Testing of Chemicals (1992). Method 203. Fish, Acute Toxicity Test.
Official Journal of the European Communities, L 383 A, Part C1, Acute Toxicity for Fish (29th December 1992).
Results & Conclusions
During this study there were no mortalities observed in both the dilution water control vessel and the test substance vessel. Therefore the 96h LC50 was determined to be >1500 mg/L. The 96h NOEC was determined to be 1500 mg/L. The lowest nominal concentration at which there was 100% mortality was >1500 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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