Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 443-510-2 | CAS number: 738587-10-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_environmental-fate-and-pathways.png)
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Meets the criteria for classification as Reliable without restriction according to Klimisch et al (1997)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Test Guideline 301F, Ready biodegradability: Manometric respirometry.
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 443-510-2
- EC Name:
- -
- Cas Number:
- 738587-10-5
- Molecular formula:
- C26H14N10Ni2O18S4 as free acid
- IUPAC Name:
- dinickel(2+) sodium 3-carboxy-5-[(1E)-2-(7-oxido-2,6-disulfonaphthalen-1-yl)diazen-1-yl]-1H-1,2,4-triazol-1-ide 3-carboxy-5-[(1E)-2-(7-oxido-2-sulfo-6-sulfonatonaphthalen-1-yl)diazen-1-yl]-1H-1,2,4-triazol-1-ide
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Activated sludge was obtained from Buckland Sewage Treatment Works, Newton Abbot, Devon, UK on 31 October 2002. This works treats sewage of predominantly domestic origin. At the laboratory, the activated sludge was kept aerated at room temperature and the pH maintained at 7.0 ± 1.0.
Six days prior to the test the activated sludge was centrifuged, washed and resuspended in the test medium and the solids concentration determined. This sludge was then diluted in medium, added to test bottles and stirred until required for use. - Duration of test (contact time):
- 28 d
- Details on study design:
- The test was conducted using activated sludge obtained from a local sewage treatment works as the inoculum.
Triplicate test substance bottles and control (inoculum blanks), together with six positive control bottles (sodium acetate), were prepared. The experimental design consisted of two types of control: inoculum blanks and abiotic controls (poisoned flasks). The former contained viable organisms with no test susbtance or reference substance, the purpose of which was to demonstrate that there was no other carbon source in the test medium. The abiotic controls contained test substance as well as the activated sludge, however the sludge organisms were killed by the addition of HgCI2. The purpose of these controls was to ensure that test substance was present in a medium that has the same amount of particulate matter as the test bottles, so that any removal mechanisms (eg adsorption) or physical degradation (eg hydrolysis) could be accounted for.
The units containing test susbstance were dosed from a primary stock solution containing 1000 mg/l of test substance in deionised water prepared on the exposure start day. Oxygen uptake was recorded on weekdays during the 28 day experimental period. Oxygen uptake values were corrected for the inoculum blank. The biodegradation was calculated as a percentage of the measured chemical oxygen demand for the test or reference substance, as appropriate.
The pH of the test medium prior to use was measured and adjusted as necessary to 7.4 ± 0.2. The pH in several bottles was measured on day 0. The pH in all of the test bottles was measured on day 28. The study was performed in a constant temperature room to maintain the test bottle temperatures within the nominal range of 22 ± 2°C.
Reference substance
- Reference substance:
- other: Sodium acetate
Results and discussion
- Preliminary study:
- None conducted.
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- < 10
- Sampling time:
- 5 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- < 10
- Sampling time:
- 11 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- < 10
- Sampling time:
- 15 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- < 10
- Sampling time:
- 20 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- < 10
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- Points of degradation plot (reference substance):
60 % degradation after 5 d
63 % degradation after 10 d
63 % degradation after 15 d
63 % degradation after 20 d
63 % degradation after 28 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- Based on the results of the test, the test substance is considered to be not readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)