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Diss Factsheets
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EC number: 203-770-8 | CAS number: 110-46-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- ''amyl nitrite'', mixed isomers
- EC Number:
- 203-770-8
- EC Name:
- ''amyl nitrite'', mixed isomers
- Cas Number:
- 110-46-3
- Molecular formula:
- C5H11NO2
- IUPAC Name:
- pentyl nitrite
Constituent 1
Test organisms
- Test organisms (species):
- Daphnia magna
Test conditions
- Hardness:
- Between 140 and 250 mg.L-1
- Test temperature:
- 20 °C ± 2
- pH:
- Between 6 and 9
- Details on test conditions:
- Light/dark cycle: 16 h/8 h
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.307 mg/L
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.307 mg/L
- Basis for effect:
- mobility
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The objective of this study was to assess the effects of the test item Isoamyl Nitrite on the mobility of Daphnia magna over a period of 48 hours under static conditions according to OECD Guideline 202 (April 2004).
Since the substance is volatile and is not soluble in under conditions of the test, the test was carried out in closed bottles and the substance was added by injection through the septum.
Test item quantification was performed according to a validated analytical method (final report RRCo-000234_01) on a GC-MS/MS from Agilent (GC 7890b and MS triple quadrupole 7000b or c) as mass spectrometry detection was found suitable to measure Isoamyl Nitrite concentrations at 0 and 48 hours.
Results are the following:
Isoamyl Nitrite
EC50 (24h) 0.317 mg.L-1 (0.307 – 0.327 mg.L-1)
EC50 (48h) 0.317 mg.L-1 (0.307 – 0.327 mg.L-1)
EC = effect concentration
The acute effects of Isoamyl Nitrite at the definitive nominal concentrations of 0; 0.31; 0.63; 1.25; 2.5 and 5 mg.L-1 to Daphnia magna were investigated under laboratory conditions.
Due to the instability of test item, the calculations were performed on the measured concentrations at initial time.
The study is valid since immobilization in the control was less than 10% (actual values: 0% in the definitive test), dissolved oxygen in the lowest concentration producing the maximum effect at the end of the test was ≥ 3 mg.L-1 (actual values: 8.5 mg.L-1 in the definitive test) and the EC50 with reference item over a period of 24 hours was between 0.6 and 2.1 mg.L-1 (actual value: 1.44 mg.L-1).
Based on the number of immobilized daphnids, the EC50 after a 24-hour exposure period was calculated to be 0.317 mg.L-1 (0.307 – 0.327 mg.L-1 95% confidence limits), and the EC50 after a 48-hour exposure period was calculated to be 0.317 mg.L-1 (0.307 – 0.327 mg.L-1 95% confidence limits).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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