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Diss Factsheets
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EC number: 604-721-7 | CAS number: 150114-71-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15 June 2001 to 30 August 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.4 (Acute Toxicity: Dermal Irritation / Corrosion)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.2500 (Acute Dermal Irritation)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: JMAFF Primary Dermal Irritation Study, 2000
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4-amino-3,6-dichloropyridine-2-carboxylic acid
- EC Number:
- 604-721-7
- Cas Number:
- 150114-71-9
- Molecular formula:
- C6H4Cl2N2O2
- IUPAC Name:
- 4-amino-3,6-dichloropyridine-2-carboxylic acid
- Test material form:
- solid: particulate/powder
- Details on test material:
- - Appearance: tan powder
Constituent 1
- Specific details on test material used for the study:
- Purity: 94.5%
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 17 - 18 weeks
- Weight at study initiation: 2.50 - 2.57 kg
- Housing: animals were individually housed in stainless steel cages
- Diet: 5 ounces of diet per day
- Water: ad libitum
- Acclimation period: at least 2 weeks
ENVIRONMENTAL CONDITIONS
- Temperature: 19 ± 3 °C
- Humidity: 40 - 60 % (relative)
- Air changes: 12 - 15 air changes per hour
- Photoperiod: 12 hours of darkness / 12 hours of light (06:00 to 18:00)
Test system
- Type of coverage:
- semiocclusive
- Preparation of test site:
- clipped
- Vehicle:
- unchanged (no vehicle)
- Remarks:
- the test material was moistened with 0.3 mL of 0.5 % aqueous methylcellulose
- Controls:
- no
- Amount / concentration applied:
- The day prior to study start, an area approximately 10 x 10 cm of the back of three New Zealand White rabbits was clipped free of fur. On day 1, a 0.5 g aliquot of the test material moistened with 0.3 mL of 0.5 % aqueous methylcellulose was applied to the back of each rabbit.
- Duration of treatment / exposure:
- 4 hours
- Observation period:
- 72 hours after test material removal.
- Number of animals:
- Three, one male and two females.
- Details on study design:
- TEST SITE
The test material was applied to the back of each rabbit and covered with a gauze patch (semi-occlusive dressing ~6 x 6 cm) with cotton backing. The gauze patch was held in place with an elastic jacket.
REMOVAL OF TEST SUBSTANCE
The jacket and patch were removed after four hours, and the back was wiped with a damp disposable towel to remove any residual test material.
SCORING SYSTEM
The application sites were graded for erythema and oedema within thirty minutes, and 24, 48, and 72 hours after test material removal. The grades for dermal lesions were assigned according to the Draize scoring system as follows:
> Erythema and eschar formation
0 = No erythema
1 = Very slight erythema (barely perceptible)
2 = Well-defined erythema (slight)
3 = Moderate to severe erythema
4 = Severe erythema (beet redness) to slight eschar formation (injuries in depth)
> Oedema formation
0 = No oedema
1 = Very slight oedema (barely perceptible)
2 = Slight oedema (edges of area well-defined by definite raising)
3 = Moderate oedema (edges raised approximately 1 mm)
4 = Severe oedema (raised more than 1 mm and extending beyond the area of exposure)
OTHER EXAMINATIONS
Animals were weighed on the day of treatment and at study termination.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- animal #1
- Time point:
- other: within 60 minutes, 24, 48 and 72 hours
- Score:
- 0
- Max. score:
- 4
- Irritation parameter:
- edema score
- Basis:
- animal #1
- Time point:
- other: within 60 minutes, 24, 48 and 72 hours
- Score:
- 0
- Max. score:
- 4
- Irritation parameter:
- erythema score
- Basis:
- animal #2
- Time point:
- other: within 60 minutes, 24, 48 and 72 hours
- Score:
- 0
- Max. score:
- 4
- Irritation parameter:
- edema score
- Basis:
- animal #2
- Time point:
- other: within 60 minutes, 24, 48 and 72 hours
- Score:
- 0
- Max. score:
- 4
- Irritation parameter:
- erythema score
- Basis:
- animal #3
- Time point:
- other: within 60 minutes, 24, 48 and 72 hours
- Score:
- 0
- Max. score:
- 4
- Irritation parameter:
- edema score
- Basis:
- animal #3
- Time point:
- other: within 60 minutes, 24, 48 and 72 hours
- Score:
- 0
- Max. score:
- 4
- Irritant / corrosive response data:
- There were no signs of dermal irritation in any of the three rabbits during the study. The rabbits were removed from study 72 hours after unwrapping.
- Other effects:
- All rabbits gained body weight over the 72-hour period.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- There were no signs of dermal irritation in any of the three rabbits during the study.
- Executive summary:
The skin irritation potential of the test material was investigated in a study which was conducted under GLP conditions and in accordance with the standardised guidelines OECD 404, EU Method B.4, EPA OPPTS 870.2500 and JMAFF (2000).
During the study aliquots of 0.5 g test material (moistened with 0.3 mL of 0.5 % aqueous methylcellulose) were applied to 1 male and 2 female New Zealand White rabbits for approximately 4 hours to the intact skin on the back of each rabbit. There were no signs of dermal irritation in any of the three rabbits during the study. The rabbits were removed from study 72 hours after unwrapping. All rabbits gained body weight over the 72-hour period.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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