Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 211-471-9 | CAS number: 646-25-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-06-23 to 2008-07-03
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- The analytical monitoring was conducted at three test concentrations (1.5, 13.6 and 60 mg/l) and in the control at the start (0h) and at the end of the test (48h). After 48h, 2 ml-samples were taken both from additional test vessels (without Daphnia) and from the test vessels with Daphnia to check the influence of the test organisms.
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: a stock solution with 33.0 mg of the test item in 495.52 ml algal growth medium was prepared and stirred for about 60 min with a magnetic stirrer. The dissolved test material resulted in a stock solution concentration of 66.6 mg/l. By diluting the test solution with the algal inoculum suspension the concentrations intended were achieved. The other test solutions were obtained by diluting the stock solution with algal growth medium
- Controls: dilution water
The recovery rates [%] for the test item in the test solutions B (1.5 mg/l), E (13.6 mg/l) and G (60 mg/l) were as follows:
At the start of the test (0h):
B: 97.3%, E: 97.1%, G: 97.8%
At the end of the test (72h):
B: 94.7%, E: 96.3%, G: 97.5% (with algae)
B: 98.7%, E: 99.3%, G: 99.8% (wihtout algae) - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISMS
- "DESMODESMUS SUBSPICATUS" CHODAT (current scientific name)
- Source/supplier: German Environmental Agency (UBA), Berlin-Marienfelde
- Laboratory culture: twice a week the stock suspension is diluted 1:17 into fresh Holm-Hansen medium under sterile conditions to keep it in exponential growth
- Light intensity: 136.4 µEm-2s-1 +/- 7.0% for 24h/day.
- Pretreatment: 3 day preculture: 3 days before the start of the test, 0.7 ml of the algae stock suspension were diluted with 49.3 ml algal growth medium (according to OECD 201) to obtain a starting conc. of 10000 cells/ml; after 3 days, the cell number was determined using a Coulter Counter Z2.
- Controls: yes, 6 replicates - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 23.6 to 23.9 degree C
- pH:
- 0 h: 7.7-9.5
72 h: 7.9-8.1 - Nominal and measured concentrations:
- Nominal concentrations A to G (0 hours): 0.7, 1.5, 3.1, 6.5, 13.6, 28.6, 60.0 mg/l
Measured concentrations (72 hours, without algae): B: 1.48, E: 13.5, G: 59.9 mg/l
Measured concentrations (72 hours, with algae): B: 1.42, E: 13.1, G: 58.5 mg/l - Details on test conditions:
- DILUTION WATER
- according to guideline
- MODIFICATIONS, if applicable
TEST SYSTEM
- Test type: static
- Exposure vessel type: sterile erlenmeyer flasks (250 ml), incubated in an temperature controlled water bath
- Test volume: 100 ml
- Number of replicates: 3, controls: 6
- Intensity of irradiation: 136.4 µEm-2s-1 +/- 7.0%
- Photoperiod: 24 h/d light
- Agitation: magnetic stirrer
TEST PARAMETER:
- "CHLOROPHYLL FLUORESCENCE" was determined at 0, 24, 48 and 72h and was measured at an exciation wave length of 465 nm and an emission wave
length of 670 nm; fluorescence values were related to cell density values according to a calibration curve. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.12 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 1.32-6.33
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 12.68 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: n.d.
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 0.76 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 0.06-1.65
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 5.78 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: n.d.
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.36 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 0.01-0.98
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 0.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 3.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- REFERENCE SUBSTANCE
- potassium dichromate
RESULTS: TEST WITH REFERENCE SUBSTANCE
- Date: 2008-01
- Results: EyC50 (Yield) = 0.X35 mg/l (required 0.20 - 0.75 mg/L); ErC50 (growth rate) = 0.80 mg/l (required 0.6 - 1.4 mg/l) - Reported statistics and error estimates:
- STATISTICS:
EC values as well as the NOEC and the LOEC were calculated with nominal concentrations using the statistical software ToxRat (ToxRat Solutions GmbH, Alsdorf). The inhibition of growth is plotted versus the concentration and a dose-response curve is calculated by probit analysis using linear Max. Likelihood regression. The ECx-values were calculated from the slope of the curve. - Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 based on growth rate was 12.68 mg/L and the NOEC was 1.5 mg/L indicating that the test substance may be harmful to algae.
- Executive summary:
In a 72 hour toxicity study, the cultures of Desmodesmus subspicatus were exposed to decamethylenediamin
at nominal concentrations of 0.7, 1.5, 3.1, 6.5, 13.6, 28.6 and 60.0 mg/L under static conditions in accordance with OECD 201. The NOEC and EC50values based on biomass (yield) were <0.7 mg/l and 3.12 mg/L, respectively. The NOEC and EC50 values based on growth rate were 1.5 mg/l and 12.68 mg/L, respectively.
There were no compound related phytotoxic effects.
This toxicity study is classified as "reliable without restrictions".
Results Synopsis
Test Organism: Desmodesmus subspicatusTest Type: Static
Growth rate:
72 hr ErC50: 12.68 mg/L 95% C.I.: n.d.
72 hr NOEC: 1.5 mg/L
Biomass (yield):
72 hr EbC50: 3.12 mg/L 95% C.I.: 1.32 to 6.33 mg/L
72 hr NOEC: <0.7 mg/L
Reference
Specific Growth Rate |
Yield 72 h nominal concentration |
|
EC50 |
12.68 mg/l |
3.12 mg/l |
95% CI |
n.d. |
1.32 – 6.33 mg/l |
EC20 |
5.78 mg/l |
0.76 mg/l |
95% CI |
n.d. |
0.06 – 1.65 mg/l |
EC10 |
3.83 mg/l |
0.36 mg/l |
95% CI |
n.d. |
0.01 – 0.98 mg/l |
Slope of the curve |
3.461 |
1.369 |
P(Chi²) |
0.973 |
0.996 |
degrees of freedom |
5 |
5 |
r² |
0.563 |
0.787 |
NOEC |
1.5 mg/l |
0.7 mg/l |
LOEC |
3.1 mg/l |
n.d. |
Description of key information
In a 72 hour toxicity study, the cultures of Desmodesmus subspicatus were exposed to decamethylenediamine at nominal concentrations of 0.7, 1.5, 3.1, 6.5, 13.6, 28.6 and 60.0 mg/L under static conditions according to TGD OECD 201. The NOEC and EC50 values based on growth rate were 1.5 mg/l and 12.68 mg/L, respectively. There were no compound related phytotoxic effects.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 12.68 mg/L
- EC10 or NOEC for freshwater algae:
- 1.5 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)