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EC number: 203-744-6 | CAS number: 110-18-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1 - 5 February 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- No chemical analysis was performed.
- Qualifier:
- according to guideline
- Guideline:
- other: USEPA TSCA 40 CFR Part 797.1400, 9/27/85, 5/20/87
- Deviations:
- not specified
- GLP compliance:
- yes
- Specific details on test material used for the study:
- TMEDA Lot number #7-0906
Clear, pale-yellow, water miscible liquid with strong ammonia odour - Analytical monitoring:
- no
- Details on sampling:
- Not applicable
- Vehicle:
- not specified
- Remarks:
- Material described as water miscible so vehicle not likely to have been used.
- Details on test solutions:
- Not details reported
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- Obtained from Musky Trout hatchery, Asbury, NJ.
Fish hatched 10 March 1987 (approx)
Tested age 121 days, less than 5% mortality during acclimation - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- Not applicable
- Post exposure observation period:
- Not applicable
- Hardness:
- Ranged between 90 and 100 mg/L as CaC03 over the duration of the study
- Test temperature:
- 12°C
- pH:
- Measured pH was concentration dependent: after 24 hours pH was 7.4, 7.8, 8.0, 8.5, 9.2 and 9.5 in the 0, 100, 180, 320, 560 and 1000 mg/L treatments respectively. From 48 hours until the end of the study pH ranged from 7.7 to 8.4 in the 0, 100, 180 and 320 mg/L treatments.
- Dissolved oxygen:
- Ranged between 10.4 and 11.0 mg/L over the duration of the study
- Salinity:
- Not applicable
- Conductivity:
- Ranged between 200 and 300 (no units provided) over the duration of the study
- Nominal and measured concentrations:
- Nominal exposure concentrations - 0 (control), 100, 180, 320, 560 and 1000 mg/L
- Details on test conditions:
- Test vessels were 4000 mL non-plasticised polypropylene containers, each containing 3000 mL of test media.
Three replicate test vessels were used test treatment, each containing 7 fish.
Aeration was supplied to each test vessel using an oil-free supply at a rate of 100 +/- 10 bubbles per minute through a pipette
Temperature, dissolved oxygen, pH and conductivity were measured daily; hardness and alkalinity were measured at the start of the study.
The study was performed with a 16 hour light and 8 hour dark cycle using fluorescent lights between 100 and 200 ft-candles.
Fish were observed for mortality and various non-lethal effects.
A sample of 25 fish had a mean weight of 528 mg, a mean length of 38.7 mm ranging from 31 – 45 mm) and a loading factor of 1.2 g of fish/L. - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 240 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Mortality after 96 hours was 0, 0, 0, 100, 100 and 100% in the control, 100, 180, 320, 560 and 1000 mg/L treatments.
There was a consistent trend of increasing mortality with time evident in the 320 mg/L treatment. Remaining concentrations had an "all or nothing" response.
No sub-lethal effects were observed in the control, 100 or 180 mg/L treatments during the study. - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- The LC50 was calculated using the modified Litchfield-Wilcoxon method.
- Sublethal observations / clinical signs:
Cumulative mortality
Nominal concentration (mg/L)
Number of fish*
Cumulative mortality (%)
24 hours
48 hours
72 hours
96 hours
Control
21
0
0
0
0
100
21
0
0
0
0
180
21
0
0
0
0
320
21
86
95
100
100
560
21
100
100
100
100
1000
21
100
100
100
100
*Total of three replicates
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96 hour LC50 for rainbow trout exposed to TMEDA was 240 mg/L, the lowest concentration causing 100% mortality was 320 mg/L and the highest concentration causing no mortality was 180 mg/L. All concentrations are reported as nominal concentrations.
- Executive summary:
Rainbow trout (Salmo gairdneri) were exposed to TMEDA at nominal test concentrations of: 0, 100, 180, 320, 560 and 1000 mg/L. This study was carried out to USEPA TSCA 40 CFR Part 797.1400, 9/27/85, 5/20/87. The study was performed as a static study with three replicates each containing seven fish, without analytical verification.
After 96 hours mortality was 0, 0, 0, 100, 100 and 100% in the nominal 0 (control), 100, 180, 320, 560 and 1000 mg/L. The 96 hour LC50 for rainbow trout exposed to TMEDA was 240 mg/L, the lowest concentration causing 100% mortality was 320 mg/L and the highest concentration causing no mortality was 180 mg/L. All concentrations are reported as nominal concentrations.
Reference
Description of key information
The short-term toxicity of TMEDA to fish was determined in a 96-hour static test performed according to USEPA TSCA 40 CFR Part 797.1400 Guideline (2011) and in compliance with GLP. The 96-h LC50 was 240 mg TMEDA/L. The lowest concentration causing 100% mortality was 320 mg/L and the highest concentration causing no mortality was 180 mg/L. All concentrations are reported as nominal concentrations.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 240 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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