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Diss Factsheets
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EC number: 201-353-5 | CAS number: 81-48-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Endpoint summary
Administrative data
Description of key information
Several in vivo skin irritation/corrosion studies according or similar to OECD guideline 404 and in vivo eye irritation studies according or similar to OECD guideline 405 are available. The test items were not irritating to the skin and not irritating to the eyes.
Key value for chemical safety assessment
Skin irritation / corrosion
Link to relevant study records
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Principles of method if other than guideline:
- Three animals were applied 500 mg of the test substance under semiocclusive conditions for 4 hours. After 4 hours the skin areas were washed with water. After 1, 24, 48, 72 hours and 7 days the skin areas were scored for erythema and edema according to Draize.
- GLP compliance:
- not specified
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- Three male rabbits with an average weight of 3.5 kg were used. The animals were housed in single cages at a room temperature of 19 - 25° C and a relative humidity of 40 - 60 %. The dark/light cycle was 12 hours. The animals received a standard nutrition and tap water ad libitum.
- Type of coverage:
- semiocclusive
- Preparation of test site:
- clipped
- Vehicle:
- water
- Controls:
- not required
- Amount / concentration applied:
- 500 mg
- Duration of treatment / exposure:
- 4 hours
- Observation period:
- 7 days
- Number of animals:
- 3 animals
- Details on study design:
- One day before the application of the test substance, an area of 6 x 6 cm of the animals was clipped. Only animals with intact skin were used. 500 mg of the test substance was applied under semiocclusive conditions for 4 hours. Afterwards the skin areas were washed with water. After 1, 24, 48, 72 hours and 7 days the skin areas were scored for erythema and edema according to Draize.
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0 at any time point
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0 at any time point
- Irritant / corrosive response data:
- Erythema and edema score was = 0 at any time point (24/48/72 hours).
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- 1-Hydroxy-4-(p-toluidino)anthraquinone is not irritating. Erythema and edema score was = 0 at any time point (24/48/72 hours).
- Executive summary:
Three animals were applied 500 mg of the test substance under semiocclusive conditions for 4 hours. After 4 hours the skin areas were washed with water. After 1, 24, 48, 72 hours and 7 days the skin areas were scored for erythema and edema according to Draize. 1-Hydroxy-4-(p-toluidino)anthraquinone is not irritating. Erythema and edema score was = 0 at any time point (24/48/72 hours).
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Principles of method if other than guideline:
- Three animals were applied 100 µg of the test substance into the conjunctival sac of one eye. The other eye served as control. The treated eye was washed after 24 hours with physiologic saline. After 1, 24, 48, 72 hours and 7 days the eyes were scored for cornea, iris, conjunctivae and chemosis according to Draize.
- GLP compliance:
- not specified
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- Three male rabbits with an average weight of 3.5 kg were used. The animals were housed in single cages at a room temperature of 19 - 25° C and a relative humidity of 40 - 60 %. The dark/light cycle was 12 hours. The animals received a standard nutrition and tap water ad libitum.
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
- Amount / concentration applied:
- 100 µl
- Duration of treatment / exposure:
- 24 hours
- Observation period (in vivo):
- 7 days
- Number of animals or in vitro replicates:
- 3 animals
- Details on study design:
- Three animals were applied 100 µg of the test substance into the conjunctival sac of one eye. The other eye served as control. The treated eye was washed after 24 hours with physiologic saline. After 1, 24, 48, 72 hours and 7 days the eyes were scored for cornea, iris, conjunctivae and chemosis according to Draize.
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0 at any time point
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Reversibility:
- other: score = 0 at any time point
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 3
- Reversibility:
- other: score = 0 at any time point
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0 at any time point
- Irritant / corrosive response data:
- Cornea opacity, iris, conjunctivae and chemosis scores were 0 at any time point (24, 48 and 72 hours).
- Other effects:
- No data
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Cornea opacity, iris, conjunctivae and chemosis scores were 0 at any time point (24, 48 and 72 hours).
- Executive summary:
Three animals were applied 100 µg of the test substance into the conjunctival sac of one eye. The other eye served as control. The treated eye was washed after 24 hours with physiologic saline. After 1, 24, 48, 72 hours and 7 days the eyes were scored for cornea, iris, conjunctivae and chemosis according to Draize. Cornea opacity, iris, conjunctivae and chemosis scores were 0 at any time point (24, 48 and 72 hours). 1-hydroxy-4-(p-toluidino)anthraquinone is not irritating to the eyes:
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Justification for classification or non-classification
In all available studies for skin irritation/corrosion or eye irritation the test items were not irritating. According to CLP classification criteria (Regulation (EC) No 1272/2008) a classification is therefore not justified.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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