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EC number: 214-987-2 | CAS number: 1241-94-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Specific investigations: other studies
Administrative data
- Endpoint:
- hepatotoxicity
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 7 February 1989 - 7 March 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study was not conducted according to a guideline, but perfomed under GLP, and acceptable basic data are given.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- To investigate the ability of 2-ethylhexyl diphenyl phosphate (2EHDPP) to cause peroxisome proliferation in the rat liver when administered in the diet, 2EHDPP was fed to groups of 5 male Fischer 344 rats at dietary levels of 0 (control), 0.1, 0.3 or 0.6%, and to groups of 6 male Sprague-Dawley rats at levels of 0 (control), 0.3 or 1.2% for 28 days. A further group of 5 F344 rats were fed 1.2% di-(2-ethylhexyl)phthalate (DEHP) for 28 days and 4 Sprague-Dawley rats were fed 1.2% DEHP for 14 days to act as positive study controls. Observations were made for variations in behavior or condition, and weight changes. At necropsy liver weight, liver biochemistry (protein, cyanide-insensitive palmitoyl Co-A) and serum analysis (triglycerides and total cholesterol) were performed.
- GLP compliance:
- yes
- Type of method:
- in vivo
Test material
- Reference substance name:
- 2-ethylhexyl diphenyl phosphate
- EC Number:
- 214-987-2
- EC Name:
- 2-ethylhexyl diphenyl phosphate
- Cas Number:
- 1241-94-7
- Molecular formula:
- C20H27O4P
- IUPAC Name:
- 2-ethylhexyl diphenyl phosphate
- Reference substance name:
- 16366-97-1
- IUPAC Name:
- 16366-97-1
- Reference substance name:
- Disflamoll
- IUPAC Name:
- Disflamoll
- Details on test material:
- - Name of test material (as cited in study report): 2-ethylhexyl diphenyl phosphate, 50/50 mixture of Santicizer 141 and Disflamoll
- Physical state: Liquid
- Storage condition of test material: Polypropylene container at room temperature
Constituent 1
Constituent 2
Constituent 3
Test animals
- Species:
- rat
- Strain:
- other: Fischer 344 and Sprague-Dawley
- Sex:
- male
Administration / exposure
- Route of administration:
- oral: feed
- Vehicle:
- unchanged (no vehicle)
- Analytical verification of doses or concentrations:
- yes
- Duration of treatment / exposure:
- 28 days
- Frequency of treatment:
- Continuous (diet)
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
0.1, 0.3, and 0.6 % 2EHDPP
Basis:
nominal in diet
Male F344: 136, 401, and 765 mg/kg bw/day resp.
- Remarks:
- Doses / Concentrations:
0.3 and 1.2 % 2EHDPP
Basis:
nominal in diet
Male Sprague-Dawley: 404 and 1610 mg/kg bw/day resp.
- No. of animals per sex per dose:
- 5 male Fischer 344/dose, and 6 male Sprague-Dawley/dose
- Control animals:
- yes, plain diet
Results and discussion
- Details on results:
- F344 rats given 0.3 and 0.6% EHDP, and Sprague-Dawley rats given 0.3 and 1,2% EHDP were lighter than their concurrent controls, and these animals all showed reduced food intake values initially, with the intake of those given 0.6 and 1.2% remaining low throughout the study. This latter effect was explained as a probable reaction to the unpalatability of the diet containing the test article.
There was a dose-related increase in both absolute and relative liver weight in both strains of rat given EHDP, but the livers showed no associated histological changes.
There were dose-related increases in serum triglyceride levels in all rats given EHDP, and F344 rats given 0.6% and Sprague-Dawley rats given 0.3 or 1,2% EHDP also showed increases in serum cholesterol levels. These changes are not associated with a peroxisome proliferator.
There were very small increases in specific palmitoyl-CoA oxidation activity (i.e. activity per mg protein) among F344 rats given 0.3 and 0.6% EHDP to 135 and 150% of control activities, resp. However, when the values were expressed per gram of liver (calculated independently of liver whole homogenate protein content), a significant increase was only observed at the 0.6% EHDP dose-level. There were no significant increases in palmitoyl-CoA oxidation activity among the Sprague-Dawley rats given EHDP.
Small decreases in hepatic whole homogenate protein content were observed in F344 rats given 0.3% EHDP and in Sprague-Dawley rats given 1.2% EHDP to 91.5% and 92.2% of the corresponding control values, resp. With both strains the small changes observed are unlikely to have any biological significance.
Any other information on results incl. tables
All the diets had a concentration of test article within the 10% range of nominal. Diets were stable for 28 days.
Applicant's summary and conclusion
- Conclusions:
- This study, investigating the ability of 2-ethylhexyl diphenyl phosphate (2EHDPP) to cause peroxisome proliferation in the rat liver when administered in the diet, could not identify a NOAEL level of 2EHDPP in terms of liver enlargement. The NOAELs for the induction of hepatic palmitoyl-CoA oxidation activity by 2EHDPP (as an index of peroxisome proliferation) were 1.2% in the diet of Sprague-Dawley rats (approx. intake of 1600 mg/kg/day) and 0.3% in the diet of Fischer 344 rats (approx. intake of 400 mg/kg/day). However, these figures were based upon only very minor changes in enzyme activities which probably have little, if any, biological significance. It is therefore thought unlikely that 2EHDPP produces peroxisome proliferation in the rat liver.
- Executive summary:
To investigate the ability of 2-ethylhexyl diphenyl phosphate (2EHDPP) to cause peroxisome proliferation in the rat liver when administered in the diet, 2EHDPP was fed to groups of 5 male Fischer 344 rats at dietary levels of 0 (control), 0.1, 0.3 or 0.6%, and to groups of 6 male Sprague-Dawley rats at levels of 0 (control), 0.3 or 1.2% for 28 days. A further group of 5 F344 rats were fed 1.2% di-(2-ethylhexyl)phthalate (DEHP) for 28 days and 4 Sprague-Dawley rats were fed 1.2% DEHP for 14 days to act as positive study controls. Observations were made for variations in behaviour or condition, and weight changes. At necropsy liver weight, liver biochemistry (protein, cyanide-insensitive palmitoyl Co-A) and serum analysis (triglycerides and total cholesterol) were performed. The effect of 2EHDPP on hepatic peroxisomes was quantified by measurement of palmitoyl Co-A oxidation.
F344 rats given 0.3 and 0.6% 2EHDPP, and Sprague-Dawley rats given 0.3 and 1,2% 2EHDPP were lighter than their concurrent controls, and these animals all showed reduced food intake values initially, with the intake of those given 0.6 and 1.2% remaining low throughout the study. This latter effect was explained as a probable reaction to the unpalatability of the diet containing the test article.
There was a dose-related increase in both absolute and relative liver weight in both strains of rat given 2EHDPP, but the livers showed no associated histological changes.
There were dose-related increases in serum triglyceride levels in all rats given 2EHDPP, and F344 rats given 0.6% and Sprague-Dawley rats given 0.3 or 1,2% 2EHDPP also showed increases in serum cholesterol levels. These changes are not associated with a peroxisome proliferator.
There were very small increases in specific palmitoyl-CoA oxidation activity (i.e. activity per mg protein) among F344 rats given 0.3 and 0.6% 2EHDPP to 135 and 150% of control activities, resp. However, when the values were expressed per gram of liver (calculated independently of liver whole homogenate protein content), a significant increase was only observed at the 0.6% 2EHDPP dose-level. There were no significant increases in palmitoyl-CoA oxidation activity among the Sprague-Dawley rats given 2EHDPP.
Small decreases in hepatic whole homogenate protein content were observed in F344 rats given 0.3% 2EHDPP and in Sprague-Dawley rats given 1.2% 2EHDPP to 91.5% and 92.2% of the corresponding control values, resp. With both strains the small changes observed are unlikely to have any biological significance.
Based on these results it is therefore thought unlikely that 2EHDPP produces peroxisome proliferation in the rat liver.
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