Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 216-032-5 | CAS number: 1477-55-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- Not stated
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets generally accepted scientific standards, well documented and acceptable for assesment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method B.4 (Acute Toxicity: Dermal Irritation / Corrosion)
- Deviations:
- yes
- Remarks:
- study conducted in the rat
- Qualifier:
- according to guideline
- Guideline:
- other: Evaluation of danger of unknown substances established by the Maritime Techniques Safety Bureau, Ministry of Transport (December 1985)
- Deviations:
- not specified
- GLP compliance:
- not specified
- Remarks:
- study predates GLP
Test material
- Reference substance name:
- m-phenylenebis(methylamine)
- EC Number:
- 216-032-5
- EC Name:
- m-phenylenebis(methylamine)
- Cas Number:
- 1477-55-0
- Molecular formula:
- C8H12N2
- IUPAC Name:
- 1-[3-(aminomethyl)phenyl]methanamine
- Test material form:
- liquid
- Details on test material:
- - Specific gravity: 1.050
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Crj-Wistar
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Crj-Wistar rats were supplied by Japan Charles River Co Ltd.
- Age at study initiation: 5 weeks; test material administered at 6 weeks
- Weight at study initiation: Initial weight not given but stated to be almost the same by sex
Test system
- Type of coverage:
- other: no covering used
- Preparation of test site:
- not specified
- Vehicle:
- unchanged (no vehicle)
- Controls:
- no
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 1mL/kg
- Concentration (if solution): Substance applied as supplied - Duration of treatment / exposure:
- 4 hours
- Observation period:
- 4 hours
- Number of animals:
- Six
- Details on study design:
- TEST SITE
- Area of exposure: 1 square centimetre on the head
REMOVAL OF TEST SUBSTANCE
- Substance was not removed
SCORING SYSTEM: Corrosiveness of test substance evaluated based on dermal changes appearing at 3 minutes, 60 minutes and 4 hours after application in accordance with the criteria established by the International Maritime Organisation.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- other: necrosis
- Basis:
- animal #1
- Remarks:
- male
- Time point:
- other: 4 hours
- Score:
- 4
- Max. score:
- 4
- Reversibility:
- no data
- Remarks:
- not reversible within time period stated
- Remarks on result:
- other: test material was not removed
- Irritation parameter:
- other: necrosis
- Basis:
- animal #2
- Remarks:
- male
- Time point:
- other: 4 hours
- Score:
- 4
- Max. score:
- 4
- Reversibility:
- no data
- Remarks:
- Not reversible within time period stated
- Remarks on result:
- other: test material was not removed
- Irritation parameter:
- other: necrosis
- Basis:
- animal #3
- Remarks:
- male
- Time point:
- other: 4 hours
- Score:
- 4
- Max. score:
- 4
- Reversibility:
- no data
- Remarks:
- not reversible within time period stated
- Remarks on result:
- other: test material was not removed
- Irritation parameter:
- other: necrosis
- Basis:
- animal #4
- Remarks:
- female
- Time point:
- other: 4 hours
- Score:
- 4
- Max. score:
- 4
- Reversibility:
- no data
- Remarks:
- not reversible within time period stated
- Remarks on result:
- other: test material was not removed
- Irritation parameter:
- other: necrosis
- Basis:
- animal #5
- Remarks:
- female
- Time point:
- other: 4 hours
- Score:
- 4
- Max. score:
- 4
- Reversibility:
- no data
- Remarks:
- not reversible within time period stated
- Remarks on result:
- other: test material was not removed
- Irritation parameter:
- other: necrosis
- Basis:
- animal #6
- Remarks:
- female
- Time point:
- other: 4 hours
- Score:
- 4
- Max. score:
- 4
- Reversibility:
- no data
- Remarks:
- not reversible within time period stated
- Remarks on result:
- other: test material was not removed
- Irritation parameter:
- erythema score
- Basis:
- mean
- Remarks:
- both sexes
- Time point:
- other: 4 hours
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Remarks on result:
- other: erythema up to score 2 observed before 4 hours, but considered to be of low relevance due to severe necrosis early in the test.
- Irritation parameter:
- erythema score
- Basis:
- mean
- Remarks:
- both sexes
- Time point:
- 24/48/72 h
- Reversibility:
- other: see 'Remarks on results'
- Remarks on result:
- other: Experiment terminated 4 hours after application due to necrosis.
- Irritation parameter:
- edema score
- Basis:
- mean
- Remarks:
- both sexes
- Time point:
- other: 4 hours
- Score:
- 4
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Remarks on result:
- other: significant oedema observed before and at 4 hours, but considered to be of low relevance due to severe necrosis
- Irritation parameter:
- edema score
- Basis:
- mean
- Remarks:
- both sexes
- Time point:
- 24/48/72 h
- Reversibility:
- other: see 'Remarks on results'
- Remarks on result:
- other: Experiment terminated 4 hours after application due to necrosis.
- Irritant / corrosive response data:
- The individual scores for erythema, oedema, haemorrhage and necrosis are given in remarks on results including tables and figures.
- Other effects:
- Subcutaneous heamorrhage appeared after 3 minutes; brown metachromatic changes macroscopically judged to be necrosis emerged after 5 minutes. These changes showed highly necrotic appearance after 60 minutes; namely, the dark red-brown skin surfaces were slightly dried and hardened. Four hours after application, the cut surfaces showed necrosis throughout the entire dermal layers and severe heamorrhage reached the dorsal muscle.
Any other information on results incl. tables
The individual scores for erythema, oedema, haemorrhage and necrosis are shown in the following table:
|
Sex |
No |
Body Weight (g) |
3 minutes |
60 minutes |
4 hours |
|||||||||
Er |
Ed |
H |
N |
Er |
Ed |
H |
N |
Er |
Ed |
H |
N |
||||
Rat |
M |
1 |
191 |
1 |
2 |
2 |
0 |
2 |
4 |
4 |
4 |
0 |
4 |
4 |
4 |
2 |
189 |
1 |
0 |
2 |
0 |
1 |
4 |
4 |
3 |
0 |
4 |
4 |
4 |
||
3 |
190 |
1 |
0 |
2 |
0 |
1 |
3 |
2 |
4 |
0 |
3 |
3 |
4 |
||
F |
4 |
148 |
2 |
0 |
1 |
0 |
1 |
2 |
2 |
4 |
0 |
3 |
3 |
4 |
|
5 |
148 |
2 |
1 |
1 |
0 |
2 |
4 |
2 |
4 |
0 |
4 |
4 |
4 |
||
6 |
149 |
1 |
1 |
0 |
0 |
2 |
4 |
3 |
4 |
0 |
3 |
3 |
4 |
Applicant's summary and conclusion
- Interpretation of results:
- corrosive
- Remarks:
- Migrated information Criteria used for interpretation of results: other:
- Conclusions:
- The substance was judged, based on the criteria of the International Maritime Organisation, to correspond to a corrosive substance classified as Packing grade II defined in the recommendations of the United Nations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)