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Diss Factsheets
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EC number: 216-644-2 | CAS number: 1633-22-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Dichlorotricyclo[8.2.2.24,7]hexadeca-1(12),4,6,10,13,15-hexaene, mixed isomers
- EC Number:
- 249-236-8
- EC Name:
- Dichlorotricyclo[8.2.2.24,7]hexadeca-1(12),4,6,10,13,15-hexaene, mixed isomers
- Cas Number:
- 28804-46-8
- Molecular formula:
- C16H14Cl2
- IUPAC Name:
- Unknown constituents
- Test material form:
- solid: granular
1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
- Remarks:
- acetone
- Details on test solutions:
- Before conducting the preliminary range finding study, test item was checked for solubility and precipitation. Test item was found to be insoluble in reconstituted water at the concentration of 0.1 mg/mL. Further solubility was checked in dimethyl formamide (DMF), acetone and Dimethyl sulfoxide (DMSO) and found to be soluble at 33.33 (Stock A), 28.57 (Stock B) and 22.22 (Stock C) mg/mL, respectively. Maximum solubility of test item found in dimethyl formamide (DMF) and acetone. Hence, test item soluble in DMF and acetone was further serially diluted in respective solvents and checked for precipitation in the test medium.
Stock solution A was serially diluted at the concentration of 16.66 (Stock D), 8.33 (Stock E), 4.16 (Stock F), and 2.08 (Stock G) mg/mL in DMF. These stock solutions were checked for precipitation test using reconstituted water. Precipitation was observed upto the concentration of 4.16 mg/mL, while no precipitation was observed at the concentrations of 2.08 mg/mL.
Stock solution B was serially diluted at the concentration of 14.29 (Stock H), 7.14 (Stock I) and 3.57 (Stock J) mg/mL in acetone. These stock solutions were checked for precipitation test using reconstituted water. Precipitation was observed upto the concentration of 7.14 mg/mL, while no precipitation was observed at the concentrations of 3.57 mg/mL.
Hence, the Acetone was used as vehicle for the study. And the highest concentration was used 0.357 mg/L.
Test organisms
- Test organisms (species):
- Daphnia magna
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.357 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- As the study was conducted as a limit test of 0.357 mg DPX-C (Di-Cloro-Di-p-Xililene)/L and no immobility observed up to 48 h, 24 and 48 h, EC50 could not be calculated and was considered to be greater than 0.357 mg DPX-C (Di-Cloro-Di-p-Xililene)/L.
- Executive summary:
This study was performed to assess the toxic effects ofDPX-C (Di-Cloro-Di-p-Xililene) exposureonDaphnia magnaunder static conditionsaccording to the OECD guideline 202.
Based on the results of the preliminary range finding study, the main study was restricted to limit study and it was performed at the dose level of 0.357 mg/L along with control and vehicle control groups. In the main study, a 48 h static toxicity test procedure was followed, comprising six groups. Each group consisted of four replicates with 5 daphnids per replicate.
The stability of the test item in the test media was also performed during method validation (JRF Study N° 228-2-13-14896). The test media was analysed for DPX-C (Di-Cloro-Di-p-Xililene) active ingredient concentration to monitor the concentration of test solution at 0 and 48 during main study. The active ingredient concentration of the test item in the test media was within the acceptable limit (80% of nominal concentration).
As the study was conducted as a limit test of 0.357 mgDPX-C (Di-Cloro-Di-p-Xililene)/L and no immobility observed up to 48 h, 24 and 48 h, EC50could not be calculated and was considered to be greater than 0.357 mgDPX-C (Di-Cloro-Di-p-Xililene)/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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