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EC number: 209-876-0 | CAS number: 596-03-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
Data source
Reference
- Reference Type:
- publication
- Title:
- Toxicity of Rhodamine B and Fluorescein Sodium to Fish and their Compatibility with Antimycin A.
- Author:
- Leif L. Marking
- Year:
- 1 969
- Bibliographic source:
- The Progressive Fish – Culturist, Vol. 31 (3), Pg. no. 139-142, 1969.
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Short term toxicity study to Rainbow trout (Salmo gairdneri), Channel catfish (Ictalurus punctatus) and Bluegill (Lepomis macrochirus) was carried out for 96 hrs.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Disodium 2-(3-oxo-6-oxidoxanthen-9-yl)benzoate
- EC Number:
- 208-253-0
- EC Name:
- Disodium 2-(3-oxo-6-oxidoxanthen-9-yl)benzoate
- Cas Number:
- 518-47-8
- Molecular formula:
- C20H12O5.2Na
- IUPAC Name:
- disodium 3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-diolate
- Test material form:
- other: Orange reddish powder
- Details on test material:
- - Name of test material (as cited in study report): Fluorescein sodium - Molecular formula (if other than submission substance): C20-H10-O5.2Na- Molecular weight (if other than submission substance): 376.274 g/mol- Smiles notation (if other than submission substance): c1(c2c(oc3c1ccc(c3)[O-])cc(=O)cc2)c1c(cccc1)C(=O)[O-].[Na+].[Na+]- InChl (if other than submission substance): 1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)25-18-10-12(22)6-8-16(18)19(15)13-3-1-2-4-14(13)20(23)24;;/h1-10,21H,(H,23,24);;/q;2*+1/p-2- Substance type: Organic - Physical state: Solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
- Details on sampling:
- - Concentrations: The conc. of test chemical used for the study was 5 and 100 mg/l (5 and 100 ppm), respectively.- Sampling method: The dye fluorescein sodium (9-(0-carboxyphenyl)-6-hydroxy-3-isoxanthenone) was acquired from Matheson, Coleman, and Bell in a dry powder in a grade.The dye for the toxicity study was weighed separately for each vessel.Stock solutions of the water-soluble dye was prepared for concentrations of 5 and 100 p.p.m. in the antimycin-dye mixtures. Because antimycin is relatively insoluble in water, it was first dissolved in acetone and then diluted with water.
Test solutions
- Vehicle:
- yes
Test organisms
- Test organisms (species):
- other: 3 different test organisms were used for the study. The test organism include- 1.Rainbow trout (Salmo gairdneri) 2.Channel catfish (Ictalurus punctatus) and 3.Bluegill (Lepomis macrochirus)
- Details on test organisms:
- No data available
Study design
- Test type:
- not specified
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Post exposure observation period:
- LC50 value was determined at 24, 48 and 96 hrs.
Test conditions
- Hardness:
- No data available
- Test temperature:
- 12°C
- pH:
- No data available
- Dissolved oxygen:
- No data available
- Salinity:
- No data available
- Nominal and measured concentrations:
- Nominal concentrations
- Details on test conditions:
- EFFECT PARAMETERS MEASURED (with observation intervals if applicable): LC50 value was determined at 24, 48 and 96 hrs.TEST CONCENTRATIONS- Test concentrations: The conc. of test chemical used for the study was 5 and 100 mg/l (5 and 100 ppm), respectively.
- Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 4 198 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% confidence interval, the LC50 vaue is for test animal Rainbow trout.
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3 420 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% confidence interval, the LC50 vaue is for test animal Rainbow trout.
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1 372 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% confidence interval, the LC50 vaue is for test animal Rainbow trout.
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3 828 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% confidence interval, the LC50 vaue is for test animal Channel catfish.
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 2 826 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% confidence interval, the LC50 vaue is for test animal Channel catfish.
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 2 267 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% confidence interval, the LC50 vaue is for test animal Channel catfish.
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 5 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% confidence interval, the LC50 vaue is for test animal Bluegill.
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 4 898 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% confidence interval, the LC50 vaue is for test animal Bluegill.
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3 433 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% confidence interval, the LC50 vaue is for test animal Bluegill.
- Reported statistics and error estimates:
- No data available
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Table: Toxicity of fluorescein sodium to test fishes at 12°C.
Dye
Species
LC50 and 95% confidence interval (ppm) at:
24 hrs
48 hrs
96 hrs
Fluorescein sodium
Rainbow trout
Channel catfish
Bluegill
4,198
(3,695 – 4,763)
3,828
(3,560 – 4,115)
5,000
(4,308 – 5,800)
3,420
(3,000 -3,900)
2,826
(2,559 – 3,123)
4,898
(4,153 – 5,765)
1,372
(1,023 – 1,840)
2,267
(1,928 – 2,670)
3,433
(2,922 – 4,028)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Based on mortality of test organism Rainbow trout, Channel catfish and Bluegill, the 24, 48 and 96 hrs LC50 values was given-Rainbow trout: LC50 values at24 hrs - 4198 mg/l48 hrs - 3420 mg/l96 hrs –1372 mg/lChannel catfish: LC50 values at24 hrs -3828 mg/l48 hrs -2826 mg/l96 hrs –2267 mg/lBluegill: LC50 values at24 hrs -5000 mg/l48 hrs -4898 mg/l96 hrs –3433 mg/l
- Executive summary:
Short term toxicity study to 3 different species of fish was carried out for 96 hrs.The test organism include-
1. Rainbow trout (Salmo gairdneri)
2. Channel catfish (Ictalurus punctatus) and
3. Bluegill (Lepomis macrochirus)
The dye fluorescein sodium (9-(0-carboxyphenyl)-6-hydroxy-3-isoxanthenone) was acquired from Matheson, Coleman, and Bell in a dry powder in a grade.
The dye for the toxicity study was weighed separately for each vessel.
Stock solutions of the water-soluble dye was prepared for concentrations of 5 and 100 p.p.m. in the antimycin-dye mixtures. Because antimycin is relatively insoluble in water, it was first dissolved in acetone and then diluted with water.
The conc. of test chemical used for the study was 5 and 100 mg/l (5 and 100 ppm), respectively.
The toxicity study was performed at temperature of 21°C. LC50 value was determined at 24, 48 and 96 hrs.
Based on mortality of test organism Rainbow trout, Channel catfish and Bluegill, the 24, 48 and 96 hrs LC50 values was given-
Rainbow trout:LC50 values at
24 hrs - 4198 mg/l
48 hrs - 3420 mg/l
96 hrs –1372 mg/l
Channel catfish:LC50 values at
24 hrs -3828 mg/l
48 hrs -2826 mg/l
96 hrs –2267 mg/l
Bluegill:LC50 values at
24 hrs -5000 mg/l
48 hrs -4898 mg/l
96 hrs –3433 mg/l
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