Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-544-6 | CAS number: 96-91-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from Water Resources Bulletin
Data source
Reference
- Reference Type:
- publication
- Title:
- Acute Toxicity of Picric Acid and Picramic Acid to Rainbow Trout, Salmo Gairdneri, and American Oyster, Crassostrea Virginica’
- Author:
- William L. Goodfellow, Jr., Dennis T. Burton, William C. Graves, Lenwood W. Hall, Jr., and Keith R. Cooper
- Year:
- 1 983
- Bibliographic source:
- Water Resources Bulletin, VOL. 19, NO. 4-641-648, AUGUST 1983
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as below
- Principles of method if other than guideline:
- Standard Methods (APHA, et al., 1981).
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 2-amino-4,6-dinitrophenol
- EC Number:
- 202-544-6
- EC Name:
- 2-amino-4,6-dinitrophenol
- Cas Number:
- 96-91-3
- Molecular formula:
- C6H5N3O5
- IUPAC Name:
- 2-amino-4,6-dinitrophenol
- Test material form:
- other: Dark red needles
- Details on test material:
- - Name of test material (as cited in study report): Picramic acid
- Molecular formula (if other than submission substance): C6H5N3O5
- Molecular weight (if other than submission substance): 199.125g\mol
- Smiles notation (if other than submission substance): c1(cc(cc(c1O)N)[N+](=O)[O-])[N+](=O)[O-]
- InChl (if other than submission substance): 1S/C6H5N3O5/c7-4-1-3(8(11)12)2-5(6(4)10)9(13)14/h1-2,10H,7H2
- Substance type: Organic
- Physical state: Dark red needles
- Analytical purity: No data available.
- Lot/batch No.:38C-0117
- Storage condition of test material: The stock solutions were stored in the dark at 5°C.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- yes
Test organisms
- Test organisms (species):
- other aquatic mollusc: Crassostrea virginica
- Details on test organisms:
- TEST ORGANISM
- Common name: American Or Virginia Oyster)
- Strain: virginica
- Source: Wilde Oyster Hatchery, Shady Side, Maryland
- Age at study initiation (mean and range, SD): Clutchless
- Weight at study initiation (mean and range, SD): No data available
- Length at study initiation (length definition, mean, range and SD): No data available
- Valve height at study initiation, for shell deposition study (mean and range, SD): 48.4 + 6.2 mm
- Peripheral shell growth removed prior to test initiation: No data available
- Method of breeding: No data available
- Feeding during test: Yes
- Food type: starch-yeast solution
- Amount: Fed 500 ml of 1.5 percent starch-yeast solution
- Frequency: Daily
ACCLIMATION
- Acclimation period: 2weeks
- Acclimation conditions (same as test or not): Yes
- Type and amount of food: Fed natural plankton in the estuarine water system supplemented with 1.5 percent corn starch-yeast solution when necessary.
- Feeding frequency: As per requirement.
- Health during acclimation (any mortality observed): Less than 3 percent mortality occurred in thelaboratory populations of oysters during acclimation.
Study design
- Test type:
- static
- Water media type:
- saltwater
- Total exposure duration:
- 144 h
Test conditions
- Test temperature:
- 10.5°C
- pH:
- 7.6
- Salinity:
- 7.0
- Nominal and measured concentrations:
- 0, 5, 15, 25, 35, and 75 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 38 l
- Aeration: Air-lift system was provided.
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): 24 hour
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates):6 concentration (one replicate)
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: No
- Intervals of water quality measurement:3 days
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 12 hlight:12 h dark
Results and discussion
Effect concentrations
- Duration:
- 144 h
- Dose descriptor:
- LC50
- Effect conc.:
- 69.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
Applicant's summary and conclusion
- Conclusions:
- LC50 was found to be 69.8 mg/l for Picramic acid in Crassostrea virginica for 144 hour
- Executive summary:
In Long-term toxicity to aquatic invertebrates for Picramic acid was determined in Crassostrea virginica for 144 hour. The LC50 was found to be 69.8 mg/l for Picramic acid.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)