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Diss Factsheets
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EC number: 939-489-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Endpoint summary
Administrative data
Description of key information
Additional information
Short-term toxicity data are available for all three trophic levels. The most sensitive species was Daphnia magna (48 -h EC50 = 34.1 mg/L; BASF SE, 2011; project no. 50E0117/10E112). The Reaction product of 2,4-Dinitrotoluene and 2,6-Dinitrotoluene and hydrogen is assessed to be acutely harmful for aquatic organisms.
Short -term toxicity to fish
In a GLP study according to OECD 203, the acute fish toxicity of the Reaction product of 2,4-Dinitrotoluene and 2,6-Dinitrotoluene and hydrogen to the zebrafish (Danio rerio) was studied. The test concentrations were analytically verified and remained within 20% of the nominal values. Based on nominal concentrations, the 96 -h LC50 is greater than 120 mg/L. The 96 -h NOEC was determined to be 120 mg/L (BASF SE, 2011; Project No. 17F117/10E120).
With high probability acutely not harmful to fish.
Long-term toxicity to fish
In Annex IX Section 9.1.6 column 2 of Regulation (EC) No 1907/2006, it is laid down that long-term toxicity testing on fish shall be proposed by the registrant if the chemical safety assessment indicates the need to investigate further the effects on fish. According to Annex I of this regulation, the chemical safety assessment triggers further action when the substance or the preparation meets the criteria for classification as dangerous according to Directive 67/548/EEC or Directive 1999/45/EC or CLP-Regulation (EC) No 1272/2008 or is assessed to be a PBT or vPvB. The hazard assessment of the substance reveals neither a need to classify the substance as dangerous to the environment, nor is it a PBT or vPvB substance, nor are there any further indications that the substance may be hazardous to the environment. In Annex XI Section 3, it is laid down that testing in accordance with sections 8.6 and 8.7 of Annex VIII and in accordance with Annex IX may be omitted, based on the exposure scenario(s) developed in the Chemical Safety Report (“Substance-Tailored Exposure-Driven Testing”). In accordance with Annex XI Section 3, it can be demonstrated in the risk assessment that the manufacture and the use of the substance do not pose an unacceptable risk for all environmental compartments as the risk characterization ratios (RCRs) of the chemical safety assessment are below 1 for all compartments (see Chemical Safety Report Ch. 9, 10).
Therefore, and for reasons of animal welfare, a long-term toxicity test on fish is not provided.
Short-term toxicity to aquatic invertebrates
In a GLP study according to OECD 202, the acute toxicity of the Reaction product of 2,4-Dinitrotoluene and 2,6-Dinitrotoluene and hydrogen to the waterflea (Daphnia magna) was studied. The test concentrations were analytically verified and remained within 20% of the nominal values. Based on nominal concentrations, the 48 -h EC50 is 34.1 mg/L. The 48 -h NOEC was determined to be 10 mg/L (BASF SE, 2011; Project No. 50E0117/10E112).
This result is supported by a screening test with Daphnia magna performed in the same laboratory under similar conditions, but without GLP conditions and analytical verification of the test substance concentrations. Based on nominal values, the EC50 was determined to be between 10 and 100 mg/L (BASF SE, 2010; Project No. 60E0117/10E119).
Acutely harmful to aquatic invertebrates.
Long-term toxicity to aquatic invertebrates
The long-term toxicity to aquatic invertebrates was investigated in a GLP study according to OECD 211 using Daphnia magna test species (BASF SE 2013; report no. 51E0117/10E132). The test concentrations were analytically determined. Reproduction was the most sensitive endpoint. The 21 -d NOEC was 3.2 mg/L, the 21 -d EC10 was 4.7 mg/L (nominal).
Based on the experimentally determined EC10 the substance is assessed to be chronically not harmful to aquatic invertebrates.
Toxicity to aquatic algae and cyanobacteria
In a GLP study according to OECD 201, the growth inhibition of the Reaction product of 2,4-Dinitrotoluene and 2,6-Dinitrotoluene and hydrogen to unicellular green algae (Desmodesmus subspicatus) was tested. The test concentrations were analytically verified. They remained within 20% of the nominal values, therefore the effect values are given based on nominal concentrations. Based on growth rate inhibition, the 72 -h ErC50 is greater than 220 mg/L. The 72 -h ErC10 was determined to be 75.6 mg/L (BASF SE, 2011; Project No. 60E0117/10E119).
With high probability acutely not harmful to aquatic algae.
Toxicity to microorganisms
The key study comes from a GLP guideline study following OECD TG 209 (2010). Domestic activated sludge was exposed to Reaction product of 2,4-Dinitrotoluene and 2,6-Dinitrotoluene and hydrogen (CAS no. 13897 -55 -7 and CAS 13897 -56 -8) for 3 h. The EC50 was 870 mg/L, the EC20 was 180 mg/L and the EC10 was 77 mg/L (BASF, 2012).
It can be concluded that the inhibition of the degradation activity of activated sludge is not anticipated when introduced in appropriately low concentrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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