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Diss Factsheets
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EC number: 202-858-3 | CAS number: 100-50-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997-09-26 till 1997-10-22
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: Official Journal of the EC L 133 Part C: Biodegradability: Examination of the respiratory inhibition
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Principles of method if other than guideline:
- A defined amount of activated sludge is mixed with synthetic medium and measured the rate of respiration. This respiration rate is compared with those in the test preparations with different concentrations of the test substance be measured. The sensitivity of the activated sludge is examined with 3.5-dichlorophenol as a reference.
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Type and Origin: Activated sludge (mixed population of various micro-organisms) from a laboratory facility with the municipal waste water is mainly operated (southern Wupperverband).
Proparation of microbial inoculum:
- not pretreatment
- pH of the culture solution before Depletion: 7.6
- adjusted actived sludge was 2 g/L (dry weight) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2 °C
- pH:
- 7.1 - 7.7
- Dissolved oxygen:
- Experiments with test substance: 3.4 - 8.1 mg/L
Experiments with reference substance: 3.8 - 9.3 mg/L
Control experiments: 3.0 - 6.5 mg/L
Physico-chemical oxygen consumption control: 9.8 mg/L - Nominal and measured concentrations:
- - Nominal concentration of test substance: 100, 180, 320, 560 and 1000 mg/L
- Nominal concentration of reference concentration: 2.5, 5, 10, 20 and 40 mg/L - Details on test conditions:
- - Two control whitout test substance at test start and test end were performed in presence of activated sludge
- One physico-chemical oxygen consumption was carried out at the hightest test concentration of 1000 mg/L in absence of activated sludge.
A synthetic medium was made by dissolving the following amounts of substance in 1 litre of water.
64 g peptone
44 g meat extract
12 g urea
2.8 g NaCl
1.6 g CaCl2 x 2H2O
0.8 g MgSO4 x 7 H2O
11.2 g K2PO4 - Reference substance (positive control):
- yes
- Remarks:
- 3.5-Dichlorophenol (purity: 97%)
- Duration:
- 3 h
- Dose descriptor:
- other: EC05
- Effect conc.:
- 35 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 6.2 - 72
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 62 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 17 - 111
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 473 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 340 - 758
- Duration:
- 3 h
- Dose descriptor:
- other: EC90
- Effect conc.:
- 3 620 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 1700 - 21400
- Duration:
- 3 h
- Dose descriptor:
- other: EC95
- Effect conc.:
- 6 440 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 2580 - 57600
- Validity criteria fulfilled:
- yes
- Remarks:
- respiratory rates of the 2 controls differ less than 15 % from each other. EC 50 of the reference compond 3.5-Dichlorophenol was in the range 5 - 20 mg/L.
- Conclusions:
- The respiration inhibition on activated sludge whitin 3 hours was measured at EC 50 of 473 mg/L.
- Executive summary:
The potenial toxic effect of cyclohex-3-ene-1-carbaldehyde was tested according to official journal of the EC L 133 Part C, which is equal to OECD Guideline 209. The respiration inhibition on activated sludge whitin 3 hours was measured at EC 50 of 473 mg/L.
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- August 1979 til Februar 1983
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Reliable with restrictions.
- Qualifier:
- according to guideline
- Guideline:
- other: Zellvermehrungstest nach Bringmann & Kühn (1977, page 87-98)
- Deviations:
- no
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- pre-dissolved with ethanol
- Test organisms (species):
- Pseudomonas putida
- Test type:
- static
- Water media type:
- freshwater
- Nominal and measured concentrations:
- Nominal concentration: 1.95 mg/L - 1000 mg/L
- Duration:
- 16 h
- Dose descriptor:
- other: EC3
- Effect conc.:
- 66 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Cell proliferation inhibition
Referenceopen allclose all
Test substance: 1,2,5,6 -Tetrahydrobenzaldehyd
Test concentration[mg/L] | Respiration rate[mg/L*h] | Inhibition[%] |
100 | 26.0 | 16.7 |
180 | 22.8 | 26.9 |
320 | 18.9 | 39.4 |
560 | 14.0 | 55.1 |
1000 | 10.0 | 67.9 |
Exposure period: 16 - 18 hours
Description of key information
The respiration inhibition on activated sludge whitin 3 hours was measured at EC 50 of 473 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 473 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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