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Diss Factsheets
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EC number: 203-604-4 | CAS number: 108-67-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP, near guideline, experimental study, published in peer reviewed literature; minor restrictions in design and/or reporting but otherwise adequate for assessment.
- Qualifier:
- according to guideline
- Guideline:
- other: In accordance with the “Provisional Procedure: extended toxicology test with Daphnia magna (determination of NOEC for reproduction rate, mortality and the time of first appearance of offspring; 21d)” as of 1 January 1984, Federal Environmental Agency.
- Deviations:
- not specified
- Principles of method if other than guideline:
- 21 day semi-static closed vessel Daphnia reproduction test. Conducted in line with the provisional procedure proposed by the West German Federal Environmental Agency
- GLP compliance:
- no
- Remarks:
- No data reported
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No data reported - Analytical monitoring:
- yes
- Details on sampling:
- The concentration in the initial preparations and in the test and blank (no test organisms or feed) preparations were determined after 48/72 hours. Samples taken twice during the test period from selected concentrations and analysed, once before the 7th day and once between the 16th and 21st day.
- Vehicle:
- no
- Details on test solutions:
- Substances tested firstly fully diluted using magnetic stirrers for 24h. Graduated dilutions prepared from stock solutions.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- 20-30 specimens in forty 2-L beakers, filled with at least 1.6 L Berlin tap water. 24 h-old animals. Culture area regulated at 20°C.
- Test type:
- other: semi-static, closed vessels
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Post exposure observation period:
- None
- Hardness:
- German hardness 16°
- Test temperature:
- 25±1°C
- pH:
- pH was never lower than the 7.0
- Dissolved oxygen:
- Average minimum oxygen saturation value of 58%.
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Stock solution concentration: 63 mg/L.
Dilution steps corresponded to a ratio of 1:2. - Details on test conditions:
- Four test vessels per concentration and four controls. 20 animals per concentration level. Feeding took place on the days when parent animals were transferred. Overall COD of 15-20 mg/L as the daily feed amount. Lit from 7am to 4pm with fluorescent lamps - Philips TL 40/25W.
Observations: Dead parent animals removed. Offspring counted, total number for each test vessel was recorded. pH value and oxygen concentration measured in two test vessels per concentration level. - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.4 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- Chemical analysis showed a loss of 1,3,5-trimethylbenzene of more than 20%. No chemical analysis was available for the 24h EC50.
- Results with reference substance (positive control):
- No data reported
- Reported statistics and error estimates:
- No data reported
- Validity criteria fulfilled:
- yes
- Remarks:
- see remarks on results
- Conclusions:
- The minimum 21 day NOEC value was determined to be 0.4 mg/L based on chemical analysis. 1,3,5-trimethylbenzene was most sensitive to reproduction rate.
- Executive summary:
This study is not GLP compliant but follows a standard guideline. There are limitations in the design and reporting but it is suitable for use for this endpoint. The minimum 21 day NOEC value was determined to be 0.4 mg/L based on chemical analysis. 1,3,5-trimethylbenzene was most sensitive to reproduction rate.
Reference
Validity criteria met: reproduction rate per parent animal after 21 days was 68.0 offspring. Parent animal mortality after 21 days was 9.1%. The pH was never lower than the 7.0 in any of the control or test preparations. Average minimum oxygen saturation vale of 58%.
Description of key information
The 21 day NOEC value was determined to be 0.4 mg/L based on chemical analysis. 1,3,5-trimethylbenzene was most sensitive to reproduction rate.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.4 mg/L
Additional information
Based on the information available, the study by Kühn et al (1981) is considered to be the key study. The study is not GLP compliant but follows a standard guideline. There are limitations in the design and reporting but it is considered suitable for use as the key study for this endpoint. The 21 day NOEC value for Daphnia magna exposed to 1,3,5-trimethylbenzene was determined to be 0.4 mg/L based on chemical analysis. 1,3,5-trimethylbenzene was most sensitive to reproduction rate.
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