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EC number: 262-104-4 | CAS number: 60207-90-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 20 Jul 1981 to 03 Aug 1981
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The vascular freshwater plant duckweed was exposed to the test item for 14 days. The increase or decrease of frond production was determined at various time intervals.
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- yes
- Remarks:
- Acetone
- Test organisms (species):
- Lemna gibba
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Reference substance (positive control):
- no
- Duration:
- 14 d
- Dose descriptor:
- EC10
- Effect conc.:
- 2.94 mg/L
- 95% CI:
- > 1.26 - <= 2.03
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- frond number
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- 9.02 mg/L
- 95% CI:
- >= 4.88 - <= 13.18
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- frond number
- Duration:
- 14 d
- Dose descriptor:
- EC90
- Effect conc.:
- 21.87 mg/L
- 95% CI:
- >= 11.72 - <= 40.8
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- frond number
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- In a toxicity study on the freshwater duckweed species Lemna gibba, performed without a standard guideline, the 14d-EC10 and EC50 values for frond production were calculated to be 2.94 mg/L and 9.02 mg/L, respectively, based on initial measured concentrations.
- Executive summary:
The toxicity of the test substance to the freshwater duckweed species Lemna gibba, was tested without a standard guideline but in compliance with GLP criteria. Triplicate 7-day-old cultures were employed for each test concentration and the controls. The test duckweed were exposed to the test substance in a static system for 14 days. The range of nominal concentrations tested was 0 (blank control), 0 (solvent control), 3, 6, 12, 25 and 50 mg/L. Cultures were incubated at 24 °C under approximately 10,300 lux illumination. Measured concentrations in the samples taken at the start of exposure were ND, ND, 2.54, 6.50, 12.9, 23.4 and 46.9 mg/L at nominal test concentrations of 0 (blank control), 0 (solvent control), 3, 6, 12, 25 and 50 mg/L, respectively. Measured concentrations ≥ 6.50 mg/L significantly affected the growth of Lemna gibba after 14 days of exposure. Inhibition of frond production was from 4 % in cultures exposed to 2.54 mg/L to 93 % in cultures exposed to 46.9 mg/L. Given these results, the 14d-EC10 for frond production was calculated to be 2.94 mg/L with a 95% confidence interval of 1.26 to 2.03 mg/L, and the 14d-EC50 for frond production was calculated to be 9.02 mg/L with a 95% confidence interval of 4.88 to 13.18 mg/L. Effects were based on initial measured concentrations.
Reference
Analytical results: Measured concentrations in the samples taken at the start of exposure were ND, ND, 2.54, 6.50, 12.9, 23.4 and 46.9 mg/L at nominal test concentrations of 0 (blank control), 0 (solvent control), 3, 6, 12, 25 and 50 mg/L, respectively. Measured levels ranged from 85 % to 108 % of the nominal levels.
Biological results: Measured concentrations of ≥ 6.50 mg/L significantly affected growth of Lemna gibba after 14 days of exposure. Inhibition of frond production was from 4 % in cultures exposed to 2.54 mg/L to 93 % in cultures exposed to 46.9 mg/L. Flowering was not observed among any of the control plants or plants exposed to the test material during the 14 day exposure period. The 14-day EC10 and EC50 and their 95 % confidence limits were calculated by a probit analysis.
Description of key information
14-d EC10 = 2.94 mg/L (mean measured concentration) and 14-d EC50 = 9.02 mg/L (mean measured concentration); frond number; Lemma gibba; freshwater species; no guideline followed; Hollister 1981
Key value for chemical safety assessment
- EC50 for freshwater plants:
- 9.02 mg/L
- EC10 or NOEC for freshwater plants:
- 2.94 mg/L
Additional information
One GLP study is available for this endpoint and included as a supporting study. Triplicate 7-day-old freshwater duckweed Lemna gibba was employed for each test concentration and the controls. The test duckweed was exposed to the test substance in a static system for 14 days. The range of nominal concentrations tested was 0 (blank control), 0 (solvent control), 3, 6, 12, 25 and 50 mg/L (initial measured concentrations ND, ND, 2.54, 6.50, 12.9, 23.4 and 46.9 mg/L, respectively). Cultures were incubated at 24 °C under approximately 10,300 lux illumination. The 14d-EC10 for frond production was calculated to be 2.94 mg/L and EC50 for frond production was calculated to be 9.02 mg/L, based on initial measured concentrations (Hollister 1981, no standard guideline followed, Reliability 2).
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