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Diss Factsheets
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EC number: 215-119-5 | CAS number: 1303-36-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24/06/2015 - 07/07/2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.42 (Skin Sensitisation: Local Lymph Node Assay)
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Diarsenic triselenide
- EC Number:
- 215-119-5
- EC Name:
- Diarsenic triselenide
- Cas Number:
- 1303-36-2
- Molecular formula:
- As2Se3
- IUPAC Name:
- diarsenic triselenide
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- - Batch: E15-01025
- Purity: 100%
- Physical state / Appearance: black powder
- Storage Conditions: oom temperature in the dark
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA/Ca
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: Harlan Laboratories B.V., Horst, The Netherlands
- Females (if applicable) nulliparous and non-pregnant: yes
- Age at study initiation: 8-12 weeks
- Weight at study initiation: 15-23g
- Housing: On receipt the animals were randomly allocated to cages.
- Diet (e.g. ad libitum): Free access to and food (2014C Teklad Global Rodent diet supplied by Harlan Laboratories UK Ltd.,Oxon, UK)
- Water (e.g. ad libitum): Free access to mains tap water
- Acclimation period: min 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19-25
- Humidity (%): 30-70
- Air changes (per hr): 15x
- Photoperiod (hrs dark / hrs light): 12/12 (6,00-18,00h)
Study design: in vivo (LLNA)
- Vehicle:
- propylene glycol
- Remarks:
- This vehicle was chosen as it produced the highest concentration that was suitable for dosing.
- Concentration:
- - Preliminary screening test: no clinical signs of toxicity were noted at a concentration of 50% w/w, this concentration was selected as the highest dose investigated in the main test of the Local Lymph Node Assay.
- 50 µL (25 µL per ear) of the test item as a suspension in propylene glycol at concentrations of 50%, 25% or 10% w/w. - No. of animals per dose:
- 3 groups of 4 animals
- Details on study design:
- - application to the dorsal surface of the ear for 3 consecutive days
- Five days following the first topical application of the test item or vehicle (Day 6) all mice were injected via the tail vein with 250 \iL of phosphate buffered saline (PBS) containing 3H-methyl thymidine (3HTdR: 80 jj-Ci/mL, specific activity 2.0 Ci/mmoL, ARC UK Ltd) giving a total of 20 jiCi to each mouse.
- control: treatment with propylene glycol alone
Results and discussion
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Value:
- 0.83
- Test group / Remarks:
- concentration in propylene glycol: 10 %w/w
- Parameter:
- SI
- Value:
- 1.24
- Test group / Remarks:
- concentration in propylene glycol: 25 %w/w
- Parameter:
- SI
- Value:
- 1.18
- Test group / Remarks:
- concentration in propylene glycol: 50 %w/w
- Cellular proliferation data / Observations:
- Black staining on the ears and fur was noted post dose on Days 1 to 3 in all test animals.
There were no deaths. No signs of systemic toxicity were noted in the test or control animals during the test.
Body weight change of the test animals between Day 1 and Day 6 was comparable to that observed in the corresponding control group animals over the same period.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The test item was considered to be a non-sensitizer under the conditions of the test.
The test item does not meet the criteria for classification according to the Globally Harmonized Classification System and to the Regulation (EC) No 1272/2008, relating to the Classification, Labelling and Packaging of Substances and Mixtures. - Executive summary:
Introduction
A study was performed to assess the skin sensitization potentialofthe test item in the CBA/Ca strain mouse following topical application to the dorsal surfaceofthe ear.
Methods
Following a preliminary screening test in which no clinical signsoftoxicity were noted at a concentrationof50%w/w, this concentration was selected as the highest dose investigated in the main testofthe Local Lymph Node Assay. Three groups,eachoffour animals, were treated with 50\xL(25\xLper ear)ofthe test item as a suspension in propylene glycol at concentrationsof50%, 25%or 10%w/w. A further groupoffour animals was treated with propylene glycol alone.
Results
The Stimulation Index expressed as the mean radioactive incorporation for each treatment group divided by the mean radioactive incorporationofthe vehicle control group are as follows:
0.83 / 1.24 / 1.18
The test item was considered to be a non-sensitizer under the conditionsofthe test.
The test item does not meet the criteria for classification according to the Globally Harmonized Classification System and to Regulation (EC) No 1272/2008, relating to the Classification, Labelling and PackagingofSubstances and Mixtures.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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