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Diss Factsheets
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EC number: 203-545-4 | CAS number: 108-05-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- genetic toxicity in vivo
- Remarks:
- Type of genotoxicity: other: genotoxicity in germ cells of male mice
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non GLP, non-guideline study, published in peer reviewed literature, adequate for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Effects of vinyl acetate and acetaldehyde on sperm morphology and meiotic micronuclei in mice
- Author:
- Lahdetie J
- Year:
- 1 988
- Bibliographic source:
- Mutation Research 202, 171-178
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The testicular genotoxic effects of vinylacetate and its hydrolysis product, acetaldehyde, were studied in mice by analyzing the induction of meiotic micronuclei.
- GLP compliance:
- not specified
- Type of assay:
- other: genotoxicity in germ cells of male mice
Test material
- Reference substance name:
- Vinyl acetate
- EC Number:
- 203-545-4
- EC Name:
- Vinyl acetate
- Cas Number:
- 108-05-4
- Molecular formula:
- C4H6O2
- IUPAC Name:
- ethenyl acetate
- Details on test material:
- - Name of test material (as cited in study report): Vinylacetate
- Supplied by: Fluka AG, Buchs, Switzerland
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- other: (C57B1/6J x C3H/He)F1
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: University of Helsinki, Finland (parents from Zentralinstitut fur Versuchstierzucht GmbH, Hannover, FRG) or Zentralinstitut fur Versuchstierzucht GmbH, Hannover, FRG directly.
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- - Vehicle(s)/solvent(s) used: olive oil for vinylacetate; (cold physiological saline for acetaldehyde)
- Duration of treatment / exposure:
- Single ip dose
- Frequency of treatment:
- Single ip dose
- Post exposure period:
- 13 days
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
1000, 750, 500, 250 mg/kg bw
Basis:
other: nominal in olive oil - vinylacetate
- Remarks:
- Doses / Concentrations:
500, 375, 250, 125 mg/kg bw
Basis:
other: nominal in cold physiological saline - acetaldehyde
- No. of animals per sex per dose:
- 1, 3, 4, 4, 4 for 1000, 750, 500, 250 and 0 (olive oil) mg/kg vinyl acetate. 4, 4, 4, 7 for 375, 250, 125 and 0 (saline) mg/kg acetaldehyde. 4 for each of the positive control groups.
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- cyclophosphamide 75 mg/kg or adriamycin 6 mg/kg
Examinations
- Tissues and cell types examined:
- Preparations of 1 mm segments of seminiferous tubules, representing seminiferous epithelial stages XII-I, were made from newly divided round spermatids at stage 1 of mouse spermatogenesis.
- Details of tissue and slide preparation:
- The mice were killed 13 days after ip injection (the time interval allowed for analysis of cells exposed at the preleptotene stage of meiosis). Newly divided round spermatids at stage 1 of mouse spermatogenesis were collected using a micro-dissection technique. Preparations of 1 mm segments of seminiferous tubules were made and stained with Hoechst 33258; these represented seminiferous epithelial stages XII-I.
- Evaluation criteria:
- 1000 early spermatids per mouse were scored for the number of micronuclei.
- Statistics:
- The statistical evaluation of meiotic micronuclei was based on Poisson distribution.
Results and discussion
Test resultsopen allclose all
- Sex:
- male
- Genotoxicity:
- negative
- Remarks:
- vinylacetate
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Sex:
- male
- Genotoxicity:
- negative
- Remarks:
- acetaldehyde
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
Table 1: Meiotic micronucleus frequencies in early spermatids of mice 13 days after a single injection of vinylacetate or acetaldehyde (frequency in 1000 early spermatids)
treatment |
vinylacetate |
oli |
acetaldehyde |
sal |
cp |
adm |
|||||
dose level (mg/kg) |
1000 |
750 |
500 |
250 |
0 |
375 |
250 |
125 |
0 |
75 |
6 |
number of mice |
1 |
3 |
4 |
4 |
4 |
4 |
4 |
4 |
7 |
4 |
4 |
frequency (mean± SE) |
0 |
0.33 ± 0.33 |
2.25 ± 0.85 |
2.00 ± 1.08 |
2.00 ± 0.71 |
1.00 ± 0.71 |
1.25 ± 0.48 |
1.5 ± 0.50 |
1.57 ± 0.61 |
4.75** ± 0.75 |
4.75** ± 3.77 |
(range) |
|
0-1 |
0-4 |
0-5 |
0-3 |
0-3 |
0-2 |
0-2 |
0-4 |
2-9 |
0-16 |
** p<0.01 compared to saline controls oli = olive oil, cp =cyclophosphamide, sal = saline, adm = adriamycin |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
Neither vinylacetate nor acetaldehyde (its hydrolysis product) induced micronuclei in meiotic cells. - Executive summary:
Neither vinylacetate nor acetaldehyde (its metabolite) induced micronuclei in meiotic cells.
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