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Diss Factsheets
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EC number: 207-431-5 | CAS number: 470-82-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- No data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets generally accepted scientific standards, well documented and acceptable for assessment.
Data source
Reference
- Reference Type:
- publication
- Title:
- Salmonella Mutagenicity Test Results for 250 Chemicals
- Author:
- Haworth S et al.
- Year:
- 1 983
- Bibliographic source:
- Haworth S, Environmental Mutagenesis Supplement 1:3-142 (1983)
- Report date:
- 1983
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Cineole
- EC Number:
- 207-431-5
- EC Name:
- Cineole
- Cas Number:
- 470-82-6
- Molecular formula:
- C10H18O
- IUPAC Name:
- 1,3,3-trimethyl-2-oxabicyclo[2.2.2]octane
- Reference substance name:
- Eucalyptol
- IUPAC Name:
- Eucalyptol
- Details on test material:
- - Name of test material (as cited in study report): Cineol (Eucalyptol)
- Lot/batch No.: C22435
- Other: Sourced from Pfaltz and Bauer
Constituent 1
Constituent 2
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced liver S-9 fractions obtained from male Sprague-Dawley rats and male Syrian hamsters, injected, i.p.
- Test concentrations with justification for top dose:
- The maximum concentration dosed was 10mg/plate unless toxicity was observed at this dose level.
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: [acetone; dimethyl sulphoxide; ethanol(95%); water (distilled)]
- Justification for choice of solvent/vehicle: The solvent of choice was distilled water, DMSO was used if the chemical was insoluble. Ethanol or acetone was used if the substance was not soluble or stable in DMSO. The final choice of solvent for this substance was not reported.
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- Tested on TA1537
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- Tested on TA100 and TA 1535 without S9.
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 4-Nitro-o-phenylenediamine (NOPD)
- Remarks:
- Tested on TA98 without S9
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene (2-AA)
- Remarks:
- Used on all strains with and without S9
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation procedure
DURATION
- Preincubation period: 37°C for 48 hours in the open lab (without shaking)
SELECTION AGENT (mutation assays):
NUMBER OF REPLICATIONS: At least five doses of test substance, in addition to the concurrent solvent and positive controls were tested on each strain in the presence of S-9 mix or buffer. Three plates were used, and the experiment was repeated no less than 1 week after completion of the initial test.
NUMBER OF CELLS EVALUATED: Not reported
DETERMINATION OF CYTOTOXICITY
- Method: viability on complete medium (EGG) and reduced numbers of revertant colonies per plate and/or thinning or the absence of the bacterial lawn (CWR, EGG, SRI). If toxicity was not apparent in the preliminary toxicity determination, the highest dose tested was 10mG/plate; otherwise the upper limit of solubility was used. If toxicity was observed, the doses of test chemical were chosen so that the high dose exhibited some degree of toxicity.
OTHER: - Evaluation criteria:
- Positive controls: The positive contol chemicals were tested concurrently with each test chemical. 2-AA was tested on all strains in the presence of rat and hamster S-9. NOPD was tested on TA98 with out S9. SA was tested on TA100 and TA1535 and 9-AAD was tested on TA1537 all without S9. The concentration for each poistive control used for each strain and activation condition was selected based on dose-response curves gebnerated at the beginning of the test. All positive controls were detected and gave reproducible results.
The negative controls were non-mutagenic in all tests. - Statistics:
- The data were evaluated using analysis based on the models presented by Margolin et al [1981].
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
other: negative with and without metabolic activation system
The test substance was tested blind following the Ames test method (reverse phase mutagenicity test) using strains TA1535, TA1537, TA98 and TA100, with and without S9. The substance did not exhibit any mutagenic effects on the strains tested with or without S9.
Concurrent positive control samples gave expected and reproducible effects with and without S9 under the conditions of the test, thereby confirming the satisfactory activity of the metabolic activation system used.
Negative(solvent) controls gave expected and reproducible responses under the conditions of the test. - Executive summary:
The test substance was tested blind following the Ames test method (reverse phase mutagenicity test) using strains TA1535, TA1537, TA98 and TA100, with and without S9. The substance did not exhibit any mutagenic effects on the strains tested with or without S9.
Concurrent positive control samples gave expected and reproducible effects with and without S9 under the conditions of the test, thereby confirming the satisfactory activity of the metabolic activation system used.
Negative(solvent) controls gave expected and reproducible responses under the conditions of the test.
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