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Diss Factsheets
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EC number: 217-468-9 | CAS number: 1863-63-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27. March 2017 - 24. May 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material:
608031
- Expiration date of the lot/batch:
25. Oct. 2018
- Purity test date: not stated
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
Room temperature, in a tightyl closed vessel
- Stability under test conditions: assumed stable
- Solubility and stability of the test substance in the solvent/vehicle:
not applicable
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:
not applicable
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
none - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, NW-Lachen-Speyerdorf
- Laboratory culture: not applicable
- Method of cultivation: not applicable
- Storage conditions: not stated
- Storage length: not stated (date of collection: 21. Apr. 2017
- Preparation of inoculum for exposure: inoculum was taken from its source, washed, aerated
- Pretreatment: The sludge was filtrated, washed with tap water (2x), then washed with and re-suspended in test medium. It was then aerated until use.
- Concentration of sludge: 4040 mg suspended solids / L (dry matter), 25 mg/L for experiment
- Initial cell/biomass concentration: not determined
- Water filtered: no
- Type and size of filter used, if any: - Duration of test (contact time):
- ca. 28 d
- Initial conc.:
- ca. 34 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline
- Additional substrate: none
- Solubilising agent (type and concentration if used): none
- Test temperature: 20-25 °C
- pH: 6.8-7.6 (depending on test flask)
- pH adjusted: no
- CEC (meq/100 g): not determined
- Aeration of dilution water: no
- Suspended solids concentration: 4040 mg/L
- Continuous darkness: no
- Other:
TEST SYSTEM
- Culturing apparatus: 1500 mL flasks
- Number of culture flasks/concentration: 1
- Method used to create aerobic conditions: aeration with purified, CO2 scrubbed, moistened air
- Method used to create anaerobic conditions: not applicable
- Measuring equipment:
- Test performed in closed vessels due to significant volatility of test substance:
- Test performed in open system:
- Details of trap for CO2 and volatile organics if used:
- Other:
SAMPLING
- Sampling frequency: on day 0, 2, 4, 8, 10,14, 18, 23,2 9
- Sampling method: 1 mL of NaOH-solution from scrubber taken
- Sterility check if applicable: not applicable
- Sample storage before analysis: not stated
- Other:
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes
- Other:
STATISTICAL METHODS: none - Preliminary study:
- none performed
- Test performance:
- All validity parameteres and values were fulfilled:
IC content of test item solution in medium: 0 %
CO2 emitted by the controls: 11.5 mg/L
Difference within replicates: 1.6 %
Degradation of positive control: 8 days
Degradation in the toxicity flask on day 14: 86.8 % - Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 92
- Sampling time:
- 29 d
- Details on results:
- The 10 day window began on day 1, at its end, 82 % degradation were reached
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- When applying the 10-day-window, Ammonium Benzoate is readily biodegradable following OECD 301B/EU C.4-C.
- Executive summary:
The test item Ammonium Benzoate was tested using a concentration of nominally 20 mg
organic carbon/L (corresponding to 33.0 mg Ammonium Benzoate/L) in test medium following
OECD 301B and EU-Method C.4-C.
Aniline was chosen as positive control.
Activated sludge was used as inoculum (concentration in the test 25.0 mg dry matter/L).
The test was left running for 28 days.
All validity criteria were met. Degradation of the positive control was 62 % after 8 days.
The following data were determined for the test item Ammonium Benzoate:
10-day-window: day 1 – 11
degradation at the end of 10-day-window 82 %
degradation at the end of the test 92 %
pass level following guideline: 60% at the end of 10-day-window for pure substances
respective 60 % at the end of the test for mixtures
Therefore, when applying the 10-day-window, Ammonium Benzoate is readily biodegradable
following OECD 301B/EU C.4-C.
Reference
Description of key information
One Guideline screening study available.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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