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Diss Factsheets
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EC number: 220-864-4 | CAS number: 2921-88-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_environmental-fate-and-pathways.png)
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Purity not reported
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, adapted
- Details on inoculum:
- - Source: The aeration stage of the HRC pIc sewage treatment plant
- Preparation of inoculum for exposure: The sample was allowed to settle and the supernatant filtered through Whatman GFA filter paper (first 200 mL discarded). - Duration of test (contact time):
- 28 d
- Initial conc.:
- 2 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Test temperature: 20 ± 1°C
- Aeration of dilution water: Yes
- Continuous darkness: Yes
TEST SYSTEM
- Culturing apparatus: 250-300 mL BOD bottles (darkened glass) with ground glass stoppers
- Number of culture flasks/concentration: Duplicate
- Test performed in closed vessels due to significant volatility of test substance: Yes
SAMPLING
- Sampling frequency: 0, 5, 15, and 28 days
CONTROL AND BLANK SYSTEM
- Other: Dilution water without inoculum and dilution water with inoculum - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 22
- Sampling time:
- 28 d
- Results with reference substance:
- Sodium benzoate attained 100% degradation after 28 days
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- % biodegradation after 28 days was 22% (not readily biodegradable)
- Executive summary:
The study was conducted to assess the ready biodegradability of the test substance following OECD guideline 301D.
2 mg/L of the test substance was used. Test media were inoculated with activated sludge filtrate at the rate of 1 drop of inoculum per litre. BOD bottles were filled, by siphon, and firmly stoppered to exclude all air bubbles. Sufficient bottles were prepared to allow a single oxygen determination per bottle to be made at 0, 5, 15 and 28 days for each test medium (duplicate bottles at each sampling time). Bottles were incubated in water bath at 20 ± 1°C.
Dissolved oxygen concentrations for each test medium were determined, in duplicate, at 0, 5, 15 and 28 days by means of lead/silver galvanic cell probe.
Percentage biodegradation after 28 days for the test substance was 22%. The test substance was not considered readily biodegradable under the test conditions employed. Sodium benzoate attained 100% degradation after 28 days.
Oxygen depletions in the inoculated and non-inoculated control series were within the prescribed limits.
Reference
Description of key information
Study Type |
Study Details | Value | Guideline | Reliability |
Ready Biodegradation Study | Closed bottle test |
Not Readily Biodegradable |
OECD 301D |
2 |
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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