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EC number: 220-864-4 | CAS number: 2921-88-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Endpoint summary
Administrative data
Description of key information
Study Type |
Species |
Findings |
Guideline |
Reliability |
Buehler test |
Guinea Pigs |
Not a sensitizer |
OECD 406, EPA 81-6 |
1 |
Buehler test |
Guinea Pigs |
Not a sensitizer |
EPA 81-6 |
1 |
Buehler test |
Guinea Pigs |
Not a sensitizer |
OECD 406 |
2 |
Key value for chemical safety assessment
Skin sensitisation
Link to relevant study records
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 406 (Skin Sensitisation)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 81-6 (Skin Sensitisation)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: MAFF Dermal Sensitization Study, 1985
- Deviations:
- no
- GLP compliance:
- yes
- Type of study:
- Buehler test
- Justification for non-LLNA method:
- 1234
- Specific details on test material used for the study:
- Substance ID: TSN100759
Lot Number: 7299412
Purity: 97.6% - Species:
- guinea pig
- Strain:
- Hartley
- Sex:
- male
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: Charles River Breeding Laboratories, Inc. Portage, Michigan
- Age at study initiation: 5 weeks
- Weight at study initiation: 368.8-435.4 g
- Housing: Five per cage
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: At least one week
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 3
- Humidity (%): 40-70
- Air changes (per hr): 15
- Photoperiod (hrs dark / hrs light): 12/12 - Route:
- epicutaneous, occlusive
- Vehicle:
- unchanged (no vehicle)
- Concentration / amount:
- 0.4 mL neat
- Day(s)/duration:
- 6 hour exposure, treated once weekly for three consecutive weeks
- Adequacy of induction:
- highest concentration used causing mild-to-moderate skin irritation and well-tolerated systemically
- Route:
- epicutaneous, occlusive
- Vehicle:
- unchanged (no vehicle)
- Concentration / amount:
- A single 0.4 ml aliquot of neat DER 331 epoxy resin or 0.5% solution of DNCB (dinitro chlorobenzene) in DPGME solvent were used as positive controls.
- Day(s)/duration:
- 6 hour exposure, treated once weekly for three consecutive weeks
- Route:
- epicutaneous, occlusive
- Vehicle:
- other: DPGME
- Concentration / amount:
- 0.4 mL of 1% test substance solution
- Day(s)/duration:
- 6 hours exposure
- Adequacy of challenge:
- highest non-irritant concentration
- Route:
- epicutaneous, occlusive
- Vehicle:
- other: DPGME
- Concentration / amount:
- Fifteen naive animals were dosed; five received a single 0.4 ml aliquot of 1% solution of the test material in DPGME solvent, five received 0.4 ml of neat DER 331, and five received 0.4 ml of 0.5% DNCB.
- Day(s)/duration:
- 6 hours
- No. of animals per dose:
- Induction and challenge: 10
Positive control: 10
Naive animals: 15 - Details on study design:
- RANGE FINDING TESTS: A preliminary skin irritation screen was conducted in order to determine a slightly irritating dose, if obtainable, as well as to establish the highest non-irritating concentration of the test material. Guinea pigs were clipped free of hair, on the left and right side (and may have been clipped on their back), the day prior to dosing. The crystalline test substance was gently heated to achieve a liquid state. Single application of 0.4 mL of neat liquefied test material, or as a 0.5, 1, 3.75, 5, 10, 20, 40, or 80% solution of liquefied test substance in dipropylene glycol monomethyl ether (DPGME), was topically applied to the skin of guinea pigs for six hours in Hill Top Chambers. The following day the application sites were depilated prior to scoring. Skin irritation readings were recorded approximately 24 and 48 hours after test material removal. Multiple animals were used in verifying that 1% solution of the test substance in DPGME was the highest non-irritating dose level.
MAIN STUDY
A. INDUCTION EXPOSURE
- No. of exposures: 3
- Exposure period: Three weeks
- Test groups: One
- Control group: Positive control group and naive group
- Site: Left side
- Frequency of applications: Once per week
- Duration: 6 hour exposure
- Concentrations: Neat
B. CHALLENGE EXPOSURE
- No. of exposures: One
- Exposure period: 6 hours
- Test groups: One
- Control group: Positive control group and naive group
- Site: Left side
- Concentrations: 1%
- Evaluation (hr after challenge): 24 and 48 hours - Challenge controls:
- Neat DER 331 or 0.5% DNCB, the positive control materials, was applied to the right side of the guinea pigs.
Fifteen naive animals were dosed; five received a single 0.4 ml aliquot of 1% solution of the test material in DPGME solvent, five received 0.4 ml of neat DER 331, and five received 0.4 ml of 0.5% DNCB. - Positive control substance(s):
- yes
- Remarks:
- dinitro chlorobenzene or DER 331 epoxy resin
- Positive control results:
- Challenge application with 0.4 ml of DER 331 epoxy resin caused slight to moderate erythema, considered to reflect a hypersensitivity response, at the test site on eight of ten animals at the 48-hour read. None of the DER 331 naive animals showed any evidence of irritation, at the 48-hour read. Challenge application with 0.4 ml of 0.5% DNCB caused slight to moderate erythema, considered to reflect a hypersensitivity response, at the test site on ten of ten animals, at the 48-hour read. None of the 0.5% DNCB naive animals showed any evidence of irritation, at the 48-hour read.
- Key result
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 1%
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- no indication of skin sensitisation
- Key result
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 1%
- No. with + reactions:
- 1
- Total no. in group:
- 10
- Remarks on result:
- no indication of skin sensitisation
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- positive control
- Dose level:
- DER 331 (Neat)
- No. with + reactions:
- 4
- Total no. in group:
- 10
- Remarks on result:
- positive indication of skin sensitisation
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- positive control
- Dose level:
- DER 331 (Neat)
- No. with + reactions:
- 8
- Total no. in group:
- 10
- Remarks on result:
- positive indication of skin sensitisation
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- positive control
- Dose level:
- 0.5% DNCB
- No. with + reactions:
- 9
- Total no. in group:
- 10
- Remarks on result:
- positive indication of skin sensitisation
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- positive control
- Dose level:
- 0.5% DNCB
- No. with + reactions:
- 10
- Total no. in group:
- 10
- Remarks on result:
- positive indication of skin sensitisation
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- other: Test substance naive group
- Dose level:
- 1%
- No. with + reactions:
- 1
- Total no. in group:
- 5
- Remarks on result:
- no indication of skin sensitisation
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- other: Test substance naive group
- Dose level:
- 1%
- No. with + reactions:
- 0
- Total no. in group:
- 5
- Remarks on result:
- no indication of skin sensitisation
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- other: Positive control naive group
- Dose level:
- DER 331 (Neat)
- No. with + reactions:
- 0
- Total no. in group:
- 5
- Remarks on result:
- no indication of skin sensitisation
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- other: Positive control naive group
- Dose level:
- DER 331 (Neat)
- No. with + reactions:
- 0
- Total no. in group:
- 5
- Remarks on result:
- no indication of skin sensitisation
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- other: Positive control naive group
- Dose level:
- 0.5% DNCB
- No. with + reactions:
- 0
- Total no. in group:
- 5
- Remarks on result:
- no indication of skin sensitisation
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- other: Positive control naive group
- Dose level:
- 0.5% DNCB
- No. with + reactions:
- 0
- Total no. in group:
- 5
- Remarks on result:
- no indication of skin sensitisation
- Group:
- negative control
- Remarks on result:
- other: Negative control was not included
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- No delayed contact hypersensitivity in guinea pig
- Executive summary:
The test substance was evaluated for dermal sensitization potential using the Buehler method. The study was conducted according to OECD guideline 406 and EPA guideline 81-6. Ten male Hartley albino guinea pigs received three dermal applications of 0.4 mL of test substance, during the three-week induction period and during the challenge application two weeks after the last induction application. Five naive animals also received 0.4 mL of test substance at the challenge phase. The condition of the test sites was assessed approximately 24 and 48 hours after the challenge application. Challenge application with 0.4 mL of test substance caused erythema at the test site in only one of the ten animals, and none of the naïve animals showed any evidence of irritation, at the 48-hour read. An additional ten male Hartley albino guinea pigs/test material received three dermal applications of 0.4 mL of neat DER 331 epoxy resin or 0.5% DNCB (dinitro chlorobenzene), respectively, which are run every six months as positive controls. Five naive animals also received a 0.4 mL aliquot of neat DER 331 epoxy resin or 0.5% DNCB, respectively, at the challenge phase. Challenge application with 0.4 mL of DER 331 epoxy resin caused slight to moderate erythema, considered to reflect a hypersensitivity response, at the test site on eight of ten animals at the 48-hour read. None of the DER 331 naive animals showed any evidence of irritation, at the 48-hour read. Challenge application with 0.4 mL of 0.5% DNCB caused slight to moderate erythema, considered to reflect a hypersensitivity response, at the test site on ten of ten animals, at the 48-hour read. The animals were in apparent good health and gained weight over the study period. Therefore, under the conditions of this study, the test substance did not cause delayed contact hypersensitivity in guinea pigs.
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 81-6 (Skin Sensitisation)
- Deviations:
- no
- GLP compliance:
- yes
- Type of study:
- guinea pig maximisation test
- Specific details on test material used for the study:
- Batch: 341-HRR-153
Purity: 99.3% - Species:
- guinea pig
- Strain:
- Dunkin-Hartley
- Sex:
- female
- Route:
- intradermal
- Vehicle:
- arachis oil
- Concentration / amount:
- 25% w/v in arachis oil B.P.
- Day(s)/duration:
- Day 0
- No.:
- #1
- Route:
- epicutaneous, occlusive
- Vehicle:
- unchanged (no vehicle)
- Concentration / amount:
- 0.1 - 0.2 mL undiluted test material
- Day(s)/duration:
- Day 21
- No.:
- #2
- Route:
- intradermal
- Vehicle:
- arachis oil
- Concentration / amount:
- 75% v/v in arachis oil B.P .
- Day(s)/duration:
- Day 21
- No. of animals per dose:
- 20 test animals and 10 control animals
- Key result
- Reading:
- 1st reading
- Dose level:
- 75%
- No. with + reactions:
- 0
- Total no. in group:
- 19
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Test substance was not sensitizing to guinea pigs.
- Executive summary:
A test was performed to assess the skin contact sensitization potential of the test material in the albino guinea pig. The method used followed that described in the USA Environmental Protection Agency (E.P.A. Guidelines. The results may be used as a basis for classification and labelling under Annex VI of Council Directive 67/548/EEC (as adapted to technical progress by Commission Directive 91/325/EEC).
Twenty test and ten control animals were used for the main study. Based on the results of sighting tests, the concentrations of test material for the induction and challenge phases were selected as follows:
Intradermal Induction 25% w/v in arachis oil B.P.
Topical Induction undiluted as supplied
Topical challenge undiluted as supplied and 75% v/v in arachis oil B.P .
The test material produced a 0% (0/19) sensitisation rate and was classified as a non-sensitiser to guinea pig skin. The test material did not meet the criteria for classification as a sensitizer according to EC labelling. No risk phrase is required.
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 406 (Skin Sensitisation)
- Deviations:
- no
- GLP compliance:
- no
- Type of study:
- Buehler test
- Specific details on test material used for the study:
- Dursban Technical
Lot Number: EK 830516110
Purity: Not reported - Species:
- guinea pig
- Strain:
- Dunkin-Hartley
- Sex:
- male/female
- Route:
- epicutaneous, occlusive
- Vehicle:
- polyethylene glycol
- Concentration / amount:
- 0.3 mL at 100% (w/v)
- Day(s)/duration:
- 6 hour exposure; treatment was repeated 7 and 14 days after the initial exposure on the same site
- Adequacy of induction:
- not specified
- Route:
- epicutaneous, occlusive
- Vehicle:
- polyethylene glycol
- Concentration / amount:
- 100% (w/v)
- Day(s)/duration:
- 6 hours exposure
- Adequacy of challenge:
- not specified
- No. of animals per dose:
- Test: 12 (6M + 6F)
Control: 12 (6M + 6F) - Positive control substance(s):
- no
- Key result
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 100% (w/v)
- No. with + reactions:
- 0
- Total no. in group:
- 12
- Remarks on result:
- no indication of skin sensitisation
- Key result
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 100% (w/v)
- No. with + reactions:
- 1
- Total no. in group:
- 12
- Remarks on result:
- no indication of skin sensitisation
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- other: test group vehicle alone
- Dose level:
- 100% (w/v)
- No. with + reactions:
- 0
- Total no. in group:
- 12
- Remarks on result:
- no indication of skin sensitisation
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- other: test group vehicle alone
- Dose level:
- 100% (w/v)
- No. with + reactions:
- 0
- Total no. in group:
- 12
- Remarks on result:
- no indication of skin sensitisation
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- No delayed contact hypersensitivity in guinea pig
- Executive summary:
A study was performed to determine the skin sensitization potential of the test substance in the albino guinea pig based on the Buehler Test.
The study was designed to meet the requirements of OECD 406.
No incidence of skin reactions observed in both test and control animals.
These results indicated that the test substance does not cause delayed contact hypersensitivity in the albino guinea pig using the Buehler Test.
- Endpoint:
- skin sensitisation: in vitro
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- an in vitro skin sensitisation study does not need to be conducted because adequate data from an in vivo skin sensitisation study are available
- Justification for type of information:
- Conduct of an In vivo study is a data requirement in other geographies (Asian countries such as India, China, Japan etc.) and therefore, an in vivo study was conducted which was adequate to evaluate the endpoint.
Referenceopen allclose all
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not sensitising)
- Additional information:
Three studies performed for evaluation of skin sensitization potential of chlorpyrifos followed Buehler method, all studies concluded that the test material is non skin sensitizer.
Respiratory sensitisation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Justification for classification or non-classification
There was no evidence of dermal sensitisation in guinea pigs. The substance does not need to be classified for skin sensitisation according to EU Classification, Labelling and Packaging of Substances and Mixtures (CLP) Regulation (EC) No. 1272/2008.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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