Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 220-864-4 | CAS number: 2921-88-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Batch Number: RMM 1430
Purity: 96.4% - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0.0625, 0.125, 0.25, 0.5, 1.0 mg/L
- Sampling method: The contents of the bottles were transferred to separating funnels containing NaCl (4 g) and the bottles rinsed with dichloromethane (50 ml) which was also transferred to the separating funnel. The layers were shaken (1 minute) then following separation, the lower organic layer was drained into a round bottom flask. The aqueous layer was re-extracted with dichloromethane (50 ml) and the organic layers combined. The solvent was evaporated just to dryness (rotary film evaporator, 30°C) and any remaining solvent removed under nitrogen. The extracts were dissolved in 2,2,4-trimethylpentane and analysed by Gas Liquid Chromatography against standards prepared from test substance.
- Sample storage conditions before analysis: Frozen - Vehicle:
- yes
- Remarks:
- 1% Tween 80 acetone
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Preliminary solution in 1% Tween 80 acetone
- Controls: 1 control and 1 solvent control
- Chemical name of vehicle: Organic solvent (1% Tween 80 acetone) - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green Algae - Chlorophytes
- Strain No.: CCAP 276/20
- Source (laboratory, culture collection): Culture Centre of Algae & Protozoa c/o Freshwater Biological Association, Cumbria, U.K. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 24 ± 1°C
- pH:
- ~8
- Nominal and measured concentrations:
- 62.5, 125, 250, 500, 1000 µg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL conical flasks
- Type: Closed
- Aeration: None. Gaseous exchange and suspension of algal cells maintained by orbital shaker.
- Initial cells density: The cell densities of the control cultures at initiation, 72 hours and at termination were determined by direct counting with the aid of a haemacytometer.
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): 3
GROWTH MEDIUM
- Standard medium used: Yes (nutrient medium)
OTHER TEST CONDITIONS
- Sterile test conditions: Yes
- Photoperiod: Continuous
- Light intensity: ~7000 lux - Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 580 µg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: Nominal effect conc.: 800 µg/L
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 480 µg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: Nominal effect conc.: 660 µg/L
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 730 µg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: Nominal effect conc. (24-48 h): >1000 µg/L (highest dose tested)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 43 µg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Remarks on result:
- other: Nominal effect conc.: 62.5 µg/L
- Details on results:
- - Observation of abnormalities: All test and control cultures were inspected at 72 and 96 hours. At both these periods during the study, clumping was apparent at 500 and 1000 µg/L. No abnormalities were detected in any of the other cultures.
- The inhibitory effects on growth occurred predominantly after 48 hours exposure, hence the apparent anomaly between the ErC50 (24 - 48 h) and EbC50 (72 hand 96 h) values.
- Care should be exercised in the interpretation of these results since it was observed that exposure levels could not be maintained at near nominal values throughout the study. Mean measured concentrations were 73% of nominal at the start of the test and 6-20% of nominal at the end (mean value 14%). Adsorption may have accounted for some of the apparent losses but assimilation by the algae may have been a contributing factor, especially at 96 hours. - Validity criteria fulfilled:
- yes
- Conclusions:
- 72 h EbC50 (green algae): 580 µg/L
96 h EbC50 (green algae): 480 µg/L
24-48 h ErC50 (green algae) >730 µg/L (highest dose tested)
NOEC (green algae): 43 µg/L - Executive summary:
A study was performed to assess the inhibitory effect of the test substance on the growth of the unicellular green alga Scenedesmus subspicatus (Chodat). The study was conducted in accordance with OECD Guideline No. 201 with an exposure period of 96 hours.
Algal cultures exposed to five concentrations of test substance plus one untreated control and one solvent control were incubated on an orbital shaker under continuous illumination at 24 ± 1°C for 96 hours. Growth was monitored daily by measuring the absorbance of the cultures at 665 nm.
The following values were derived form the data, based on nominal concentrations:
EbC50 (72 h): 800 µg/L
EbC50 (96 h): 660 µg/L
ErC50 (24-48 h): >1000 µg/L
NOEC: 62.5 µg/L
Mean measured concentrations were 73% of nominal at the start ofthe test and 14% at the end. Consequently, results are also expressed in terms of initial measured concentration:
EbC50 (72 h): 580 µg/L
EbC50 (96 h): 480 µg/L
ErC50 (24-48 h): >730 µg/L
NOEC: 43 µg/L
Reference
Description of key information
72 h EbC50 (green algae, Desmodesmus subspicatus): 580 µg/L, 96 h EbC50 (green algae): 480 µg/L; OECD 201; Reliability = 2
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 480 µg/L
- EC10 or NOEC for freshwater algae:
- 43 µg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)