Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 245-119-0 | CAS number: 22605-92-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 28 Apr - 29 Jun 2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: ICCVAM recommended HET-CAM test method protocol (Nov. 2006)
- Deviations:
- no
- Principles of method if other than guideline:
- The in vitro study was performed to evaluate the irritating potential of nickel hydrogencitrate by detection of damages in blood vessels under the chorioallantoic membrane of 9 day incubated chicken eggs.
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Kaiser-Friedrich-Straße 7, 55116 Mainz, Germany
Test material
- Reference substance name:
- Nickel(2+) hydrogen citrate
- EC Number:
- 242-533-3
- EC Name:
- Nickel(2+) hydrogen citrate
- Cas Number:
- 18721-51-2
- IUPAC Name:
- hydrogen nickel(2+) citrate
- Details on test material:
- - Name of test material (as cited in study report): Nickel Hydrogencitrat
- Analytical purity: > 99%
-Batch No.: WRG 09012701037
- Storage condition of test material: 20 ± 5°C, dry
- Date of production: 27 Jan 2009
- Date of expiry: 26 Jan 2011
Constituent 1
Test animals / tissue source
- Species:
- other: Lohmann selected Leghorn chicken´s egg
- Strain:
- other: Lohmann selected Leghorn chicken´s egg
- Details on test animals or tissues and environmental conditions:
- The eggs were obtained from the LSL Rhein Main Geflügelvermehrungsbetrieb, Dieburg, Germany.
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: not applicable
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 300 µL (mean weight: 0.1847 g) - Duration of treatment / exposure:
- not applicable; see "any other information on materials and methods"
- Observation period (in vivo):
- not applicable; see "any other information on materials and methods"
- Number of animals or in vitro replicates:
- not applicable; see "any other information on materials and methods"
Results and discussion
Any other information on results incl. tables
Experimental observations and irritation scores are given in table 1 and table 2, respectively.
The negative control showed no irritating effects on the blood vessels. The calculated mean irriation score was 0.0.
Solutions of SDS (1%) and NaOH (0.1 N) were used as positive controls. The positive controls induced a severe irritation on the blood vessels. The calculated mean irriation score were 10.48 for SDS and 19.56 for NaOH.
The test item Nickel Hydrogencitrat showed effects on the blood vessels of the CAM. The calculated mean irriation score was 5.85.
Under the experimental conditions, the test item is moderately irritating to the eyes.
Table 1: experimental observations
Test Group |
Time until |
Time until |
Time until |
|
seconds |
seconds |
seconds |
Negative Control |
301 |
301 |
301 |
Negative Control |
301 |
301 |
301 |
Negative Control |
301 |
301 |
301 |
Positive Control 0.1 NaOH |
9 |
31 |
22 |
Positive Control 0.1 NaOH |
8 |
33 |
19 |
Positive Control 0.1 NaOH |
8 |
34 |
20 |
Positive Control 1% SDS |
14 |
54 |
301 |
Positive Control 1% SDS |
12 |
58 |
301 |
Positive Control 1% SDS |
15 |
59 |
301 |
Nickel Hydrogencitrat |
150 |
175 |
301 |
Nickel Hydrogencitrat |
104 |
168 |
301 |
Nickel Hydrogencitrat |
135 |
179 |
301 |
Nickel Hydrogencitrat |
139 |
171 |
301 |
Nickel Hydrogencitrat |
114 |
1701 |
301 |
Nickel Hydrogencitrat |
123 |
183 |
301 |
Calculation of the irritation score
The irritation score was calculated from the following equation:
IS= ((301 -time until haemorrhage)/300)*5 + ((301 -time until lysis)/300)*7 + ((301 -time until coagulation)/300)*9
The calculated IS for each replicate and the corresponding means are presented in the following table:
Table 2: irritation score
Test Group |
Irritation Score |
Mean Irritation Score |
Negative Control |
0.00 |
0.00 |
Negative Control |
0.00 |
|
Negative Control |
0.00 |
|
Positive Control 0.1 NaOH |
19.54 |
19.56 |
Positive Control 0.1 NaOH |
19.60 |
|
Positive Control 0.1 NaOH |
19.54 |
|
Positive Control 1% SDS |
10.55 |
10.48 |
Positive Control 1% SDS |
10.49 |
|
Positive Control 1% SDS |
10.41 |
|
Nickel Hydrogencitrat |
5.46 |
5.85 |
Nickel Hydrogencitrat |
6.39 |
|
Nickel Hydrogencitrat |
5.61 |
|
Nickel Hydrogencitrat |
5.73 |
|
Nickel Hydrogencitrat |
6.17 |
|
Nickel Hydrogencitrat |
5.72 |
Classification of the irritation potential is made as stated in the table 3:
Irritation Score (IS) |
Classification |
0 - 0.9 |
not irritant |
1 - 4.9 |
slightly irritant |
5 - 8.9 |
moderately irritant |
9 - 21 |
severely irritant |
The validity criteria and findings are given in the table 4:
Parameter |
Criterion |
Found |
Assessment |
IS of negative controls |
< 0.9 |
0.0 |
ok |
IS of positive controls |
> 9 |
19.56 (NaOH) |
ok |
Applicant's summary and conclusion
- Interpretation of results:
- moderately irritating
- Remarks:
- Migrated information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)