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EC number: 232-261-3 | CAS number: 7803-55-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 2006-09-05 till 2006-09-15
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well documented guideline study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- ammonium trivanadium octaoxide
- IUPAC Name:
- ammonium trivanadium octaoxide
- Reference substance name:
- Ammonium trivanadium octaoxide
- EC Number:
- 235-384-0
- EC Name:
- Ammonium trivanadium octaoxide
- IUPAC Name:
- 235-384-0
- Reference substance name:
- 12207-63-5
- Cas Number:
- 12207-63-5
- IUPAC Name:
- 12207-63-5
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report): "Ammoniumpolyvanadate (APV)"
- Molecular formula (if other than submission substance): (NH4)2V6O16
- Physical state: solid, yellow powder
- Storage condition of test material: at room temperature
Constituent 1
Constituent 2
Constituent 3
Method
- Target gene:
- not applicable
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 97
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- S. typhimurium TA 98
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- S. typhimurium TA 100
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- S. typhimurium TA 102
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- S. typhimurium TA 1535
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- Experiment I:
- without metabolic activation: 7, 21, 62, 185, 556 and 1667µg/plate
- with metabolic activation: 21, 62, 185, 556, 1667 and 5000µg/plate
Experiment II:
- without metabolic activation: 2.3, 7, 21, 62, 185 and 556µg/plate
- with metabolic activation: 7, 21, 62, 185, 556 and 1667µg/plate - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: A 0.5% aqueous solution of Na-carboxymethylcellulose (CMC, Lot No. 98H0328, Sigma) was used as vehicle for the test substance and for the negative control group (vehicle control, 100µl).
- Justification for choice of solvent/vehicle: The test substance was not soluble in water or DMSO.
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene; 1 or 2µg (dissolved in DMSO)
- Remarks:
- with metabolic activation; strains TA98, TA100 and TA1535
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 7,12-dimethylbenzanthracene
- Remarks:
- with metabolic activation; strain TA97a
Migrated to IUCLID6: ; 10µg (dissolved in DMSO)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 1,8-Dihydroxy-anthraquinone; 50µg (dissolved in DMSO)
- Remarks:
- with metabolic activation; strain TA102
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- without metabolic activation; strain TA98
Migrated to IUCLID6: ; 2µg (dissolved in DMSO)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- without metabolic activation; strains TA100 and TA1535
Migrated to IUCLID6: ; 1 or 2µg (dissolved in sterile water)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 4-Nitro-o-phenylenediamine; 10µg (dissolved in DMSO)
- Remarks:
- without metabolic activation; strain TA97a
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: t-Butyl-hydroperoxide; 50µg (dissolved in sterile water)
- Remarks:
- without metabolic activation; strain TA102
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar
The test substance was tested with the plate incorporation method and the results were verified by a second independent experiment.
DURATION
- Exposure duration: 48 hours at 37°C
NUMBER OF REPLICATIONS: Triplicate repetitions were run for each dose group; for the control groups 6-fold repetitions were run.
COUNTING OF COLONIES: The plates with less than about 50 revertant colonies, i.e. the plates of TA98 and TA1535 with the exception of the positive controls, were counted visually. The other plates were photographed and the picture files were scanned for colonies by a computer program.
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth:
Different concentrations of test substance suspensions were mixed with phosphate buffer or S9 mix, bacteria (TA100) and top-agar and spread over a plate with minimal agar. The plates were incubated at 37°C for 48 hours and the growth of the bacterial background and the density of revertant colonies were determined.
OTHER EXAMINATIONS:
No further examinations. - Evaluation criteria:
- A reproducible increase of the number of revertants to more than the following threshold values for at least one of the concentrations:
- For the strains with a low spontaneous revertant rate i.e. TA98 and TA1535: The 2.5-fold of the amount of the spontaneous revertants.
- For the strains with a high spontaneous revertant rate i.e. TA97a, TA100 and TA102: The 1.67-fold of the amount of the spontaneous revertants. - Statistics:
- Means and standard deviations were calculated for the number of mutants in every concentration group.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 97
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- There was no increase in the number of mutants in any of the tested bacterial strains at any of the tested concentrations. The addition of an external metabolising system did not change these results.
- Cytotoxicity / choice of top concentrations:
- other: Slight toxicity to the bacteria was noted in the higher concentrations.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- There was no increase in the number of mutants in any of the tested bacterial strains at any of the tested concentrations. The addition of an external metabolising system did not change these results.
- Cytotoxicity / choice of top concentrations:
- other: Slight toxicity to the bacteria was noted in the higher concentrations.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- There was no increase in the number of mutants in any of the tested bacterial strains at any of the tested concentrations. The addition of an external metabolising system did not change these results.
- Cytotoxicity / choice of top concentrations:
- other: Slight toxicity to the bacteria was noted in the higher concentrations.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- There was no increase in the number of mutants in any of the tested bacterial strains at any of the tested concentrations. The addition of an external metabolising system did not change these results.
- Cytotoxicity / choice of top concentrations:
- other: Slight toxicity to the bacteria was noted in the higher concentrations.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- There was no increase in the number of mutants in any of the tested bacterial strains at any of the tested concentrations. The addition of an external metabolising system did not change these results.
- Cytotoxicity / choice of top concentrations:
- other: Slight toxicity to the bacteria was noted in the higher concentrations.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Effects of pH: pH 3.79 (1% suspension in deionised water, w/v, determined with a pH-Meter WTW pH 340)
- Water solubility: The test substance was not soluble.
- Precipitation: Test substance particles were visible in the higher concentration groups when the test substance was mixed with the agar.
RANGE-FINDING/SCREENING STUDIES: The test substance was toxic to the bacteria in the samples without metabolic activation at 5000 and 1667µg/plate and in the samples with metabolic activation at 5000µg/plate. Therefore 1667µg/plate was used as the highest concentration for the samples without metabolic activation and 5000 µg/plate as the highest concentration for the samples with metabolic activation which both could be toxic and 6 concentrations were tested each. Each of the other 5 concentrations was 1/3 of the preceding one.
The concentrations for the second experiment were changed due to the results of the first one: Toxicity was noted in the 1667 and 556µg/plate samples in the plates without metabolic activation and in the 5000 and 1667 µg/plate samples in the plates with metabolic activation. Therefore the concentrations were decreased one step each.
COMPARISON WITH HISTORICAL CONTROL DATA: Threshold values for evaluation of the results were derived from the variations in the control samples of available historical data of the Ames test.
ADDITIONAL INFORMATION ON CYTOTOXICITY: Slight toxicity to the bacteria was noted in the higher concentrations: Although the bacterial background was normal in the higher concentrations, the colonies were reduced in number and size, or no growth of bacteria was noted at all. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
According to the results obtained in this study, the test substance "Ammoniumpolyvanadate (APV)" is non-mutagenic in the Ames test with the strains TA97a, TA98, TA100, TA102 and TA1535 with and without an external metabolic activation system up to the limit of toxicity. - Executive summary:
"Ammoniumpolyvanadate (APV)" was tested for mutagenic activity with the Ames test. The study was conducted in accordance with the OECD guideline 471. The test substance was suspended in 0.5% CMC. The following concentrations were tested: 7, 21, 62, 185, 556 and 1667µg/plate without metabolic activation and 21, 62, 185, 556, 1667 and 5000µg/plate with S9 mix. In a second experiment the concentrations were changed as follows: 2.3, 7, 21, 62, 185 and 556µg/plate without S9 mix and 7, 21, 62, 185, 556 and 1667µg/plate with S9 mix.
The test was performed according to the "direct plate incorporation method". The bacterial strains TA97a, TA98, TA100, TA102 and TA1535 were used. Negative and positive controls were included.
In the main test slight toxicity to the bacteria was noted in the higher concentrations: Allthough the bacterial background was normal in the higher concentrations, the colonies were reduced in number and size, or no growth of bacteria was noted at all.
In none of the concentrations tested and with none of the strains used an increase of the mutation frequency to more than the threshold values (250% of the controls for strains TA98 and TA1535, 167% of the controls for strains TA97a, TA100 and TA102) was obtained. Metabolic activation did not change these results.
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