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EC number: 619-057-3 | CAS number: 94667-33-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13/07/2000 - 24/10/2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
- Deviations:
- yes
- Remarks:
- Low humic soils biomass under 1% total organic carbon
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 217 (Soil Microorganisms: Carbon Transformation Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes
- Specific details on test material used for the study:
- No information provided.
- Analytical monitoring:
- no
- Details on sampling:
- Samples were taken to determine nitrogen metabolite content on days 5 and 28. CO2 evolution was determined on days 5 – 8 and 25 – 28.
- Vehicle:
- not specified
- Details on preparation and application of test substrate:
- Two soils were used in this study, a sandy loam soil, and a low humic content sand soil.
A sample of sandy loam soil was taken from grassland located at the Maasdijk, Heerewaarden, The Netherlands on 30 June 2000. A sample of low humic content sand soil was taken at the Bulb Research Institute, Lisse, The Netherlands on 30 June 2000. The soil samples were stored at refrigerator temperatures pending use. Soil was dried and sieved prior to testing to obtain soil granules < 2mm.
Powdered lucerne meal was added (C/N ratio 13/1) was added at a concentration of 0.3 g per 50 g dry weight and mixed thoroughly. - Test organisms (inoculum):
- soil
- Total exposure duration:
- 28 d
- Test temperature:
- 20 ± 2°C
- Moisture:
- Approximately 50% of Maximum Water Holding Capacity (MWHC)
- Details on test conditions:
- 50 ± 0.5 g dry weight of soil samples were mixed with lucerne meal and placed in 100 ml Scott Duran bottles. The samples were incubated in the dark at 20 ± 2°C. A stock solution of the test substance was created and subsequent dilutions were made in ultrapure water, 0.5 ml of which was added to the 50g dry weight soil sample to achieve a final test substance concentration. Samples were dosed with test substance at concentrations 0, 10, 100 and 1000 mg a.s./kg dry weight of soil.
At the start of the test and after 5 and 28 days, nitrite, nitrate and ammonium were extracted from the soils and were analyzed with Dr Lange test kits. Measurements were carried out using a CADAS 30 spectrophotometer.
Carbon mineralization was measured at 5 and 28 days by trapping evolved CO2 in a 0.6M sodium hydroxide solution for 72 hours and titrating it. - Nominal and measured concentrations:
- 0, 10, 100 and 1000 mg/kg dry weight of soil
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Remarks on result:
- other:
- Remarks:
- Sandy loam low humic content sand
- Details on results:
- The test substance had no effect on the production of nitrates, nitrites and carbon dioxide. The rate of ammonium production increased. The results indicate tat the test substance did not inhibit the nitrate and nitrite formation at the tested concentrations in both soil types tested after 5 and after 28 days of incubation. No significant relevant reduction effects were found between the CO2 evolution rates in treated and untreated soils. An increase in CO2 evolution in several samples may be partly caused by the biodegradable solvent in the test substance.
- Results with reference substance (positive control):
- No information provided.
- Reported statistics and error estimates:
- Two-tailed Dunnett’s test
- Validity criteria fulfilled:
- yes
- Conclusions:
- The difference in CO2 prodction and nitrogen transformation between the treated and untreated soil samples did not exceed 50% after 28 days of incubation. The highest inhibition recorded was 82.5% inhibition of nitrification in the sandy loam soil after 5 days at 10 mg/kg. However, after 28 days of incubation the inhibition was below 25%. Therefore it was not necessary to continue the test beyond 28 days.
Didecyldimethylammonium Chloride can be characterised as having no long-term influence on nitrogen or carbon transformations in soils under the conditions of this study. - Executive summary:
In a study conducted in accordance with OECD Guidelines 216 and 217, 50 g samples of low humic content sand and sandy loam were treated with Didecyldimethylammonium Chloride at concentrations of 0, 10, 100 and 1000 mg/kg dry weight soil and incubated in the dark at 20°C for 28 days. Samples were taken to determine nitrogen metabolite content on days 5 and 28. CO2 evolution was determined on days 5 – 8 and 25 – 28. Didecyldimethylammonium Chloride can be characterised as having no long-term influence on nitrogen or carbon transformations in soils. The test substance had no significant effect on the production of nitrates, nitrites and carbon dioxide.
Reference
Table 1. Nitrite formation
Dose concentration (mg/g) |
Mean nitrite formation rate (mg/kg dry weight/day) |
% reduction |
||||||
Low humic content sand |
Sandy loam |
Low humic content sand |
Sandy loam |
|||||
Day |
5 |
28 |
5 |
28 |
5 |
28 |
5 |
28 |
0 |
0.35 |
0.02 |
0.80 |
0.02 |
- |
- |
- |
- |
10 |
0.38 |
0.02 |
0.81 |
0.01 |
-8.1 |
6.7 |
-1.2 |
10.7 |
100 |
0.34 |
0.02 |
0.81 |
0.02 |
4.5 |
4.1 |
-1.4 |
0.8 |
1,000 |
0.35 |
0.06 |
0.79 |
0.02 |
0.6 |
-135.4 |
0.8 |
-9.3 |
Table 2. Ammonium formation
Dose concentration (mg/g) |
Mean ammonium formation rate (mg/kg dry weight/day) |
% reduction |
||||||
Low humic content sand |
Sandy loam |
Low humic content sand |
Sandy loam |
|||||
Day |
5 |
28 |
5 |
28 |
5 |
28 |
5 |
28 |
0 |
2.36 |
0.29 |
1.66 |
0.10 |
- |
- |
- |
- |
1,000 |
4.33 |
0.40 |
3.09 |
0.10 |
-83.8 |
-86.5 |
-39.1 |
-6.2 |
Table 3. Carbon dioxide production
Dose concentration (mg/g) |
Mean carbon dioxide formation rate (mg/kg dry weight/day) |
% reduction |
||||||
Low humic content sand |
Sandy loam |
Low humic content sand |
Sandy loam |
|||||
Day |
5-8 |
25-28 |
5-8 |
25-28 |
5-8 |
25-28 |
5-8 |
25-28 |
0 |
284.0 |
28.6 |
237.9 |
25.7 |
- |
- |
- |
- |
10 |
327.3 |
25.3 |
216.3 |
31.2 |
-15.2 |
11.6 |
9.1 |
-21.5 |
100 |
292.3 |
8.7 |
260.4 |
31.3 |
-2.9 |
69.6 |
-9.5 |
-22.0 |
1,000 |
261.9 |
104.3 |
181.6 |
31.4 |
7.8 |
-264.8 |
23.6 |
-22.3 |
Description of key information
One key study is available. The study was conducted in accordance with OECD Guidelines 216 and 217, with no deviations and was GLP compliant. Sandy loam and low humic content sand were treated with the test material Bardac 22 at concentrations of 0, 10, 100 and 1000mg/kg dry weight soil for 28 days to assess the toxicity to soil microorganisms. Didecyldimethylammonium Chloride can be characterised as having no long-term influence on nitrogen or carbon transformations in soils. The test substance had no significant effect on the production of nitrates, nitrites and carbon dioxide.
The EC50 is greater 1000 mg/kg dw, result based on nominal concentration.
Key value for chemical safety assessment
- Short-term EC50 for soil microorganisms:
- 1 000 mg/kg soil dw
Additional information
In a study conducted in accordance with OECD Guidelines 216 and 217, 50 g samples of low humic content sand and sandy loam were treated with Didecyldimethylammonium Chloride (DDAC) at concentrations of 0, 10, 100 and 1000 mg/kg dw soil and incubated in the dark at 20°C for 28 days. Samples were taken to determine nitrogen metabolite content on days 5 and 28. CO2evolution was determined on days 5 – 8 and 25 – 28. Didecyldimethylammonium Chloride can be characterised as having no long-term influence on nitrogen or carbon transformations in soils. The test substance had no significant effect on the production of nitrates, nitrites and carbon dioxide.
It should be noted that the conversion of organic nitrogen to inorganic nitrogen is a three-step sequential process which involves a wide range of microorganisms including fungi, heterotrophic bacteria and autotrophic bacteria. The results of this study indicate that all these groups remain present and functional when exposed to DDAC. The EC50 is greater 1000 mg/kg dw, result based on nominal concentration.
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