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EC number: 701-394-3 | CAS number: 1782069-81-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018-05-07 until 2018-12-21
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
- Version / remarks:
- Year 2000
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- AMENDMENT OF SOIL
- Type of organic substrate: Freshly sampled and 2mm sieved soil from the LUFA Speyer, standard soil type 2.3, silty sand (Sand content: 57.3%; pH: 5.9; organic carbon content: 0.66%)
APPLICATION OF TEST SUBSTANCE TO SOIL
- Method: Application solution (20 mL) was applied dropwise to quartz sand (40g) and carefully mixed with a mortar. After evaporation of the solvent the quartz sand-test item mixture was mixed with a soil batch of 500 g dry soil per treatment.
VEHICLE:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Acetone
- Evaporation of vehicle before use: yes - Test organisms (inoculum):
- soil
- Total exposure duration:
- 28 d
- Test temperature:
- 20 +/- 2°C
- Moisture:
- Soil water content was adjusted to about 49.32 % MWC and was was controlled weekly by weighing.
- Details on test conditions:
- TEST SYSTEM
- Test container (type, material, size): Glass incubation flasks, 1 L, stoppered with cotton wool plugs
- Amount of soil: 150g (dw)
- No. of replicates per concentration: 2
- No. of replicates per control: 2
- No. of replicates per vehicle control: 2
SOIL INCUBATION
- Method: The soil arrived on 26-Apr-2018 (DAT -54) at IES Ltd., was stored in the dark under aerobic conditions at approx. 4 °C for 28 days and then pre-moistened to approx. 40 % MWHC. Afterwards, the pre-moistened soil was acclimatised to test conditions (dark, approx. 20 °C) for 26 days until test start on 19-Jun-2018 (DAT 0). Prior to application, the soil moisture content was adjusted to 45 % MWHC.
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographical reference of sampling site (latitude, longitude): LUFA Speyer 2.3
- Vegetation cover: No cultivation 2014 - 2018
- Treatments with pesticides or fertilizers: No fertilization 2017 – 2018 and 2015 – 2016; 2014 3 x 3500 kg CaO/ha, 1463 kg MgO/ha; No pesticides 2014 - 2018
- Depth of sampling: approx. 20 cm
- Soil type: Silty sand
- % sand: 57.3%
- pH (in water): 5.9
- Initial nitrate concentration for nitrogen transformation test (mg nitrate/kg dry weight): 0.08 %
- Maximum water holding capacity (in % dry weigth): 34.9
- Cation exchange capacity (mmol/kg): 7.3
- Initial microbial biomass as % of total organic C: 3.23 (The microbial biomass of the test soil was measured by the fumigation method. The mean values of soluble total organic carbon of the fumigated soil and not fumigated soil samples were 137.5 and 56.4 mg/kg dry soil, respectively, resulting in the calculation of a microbial biomass of 213.3 mg/kg dry soil)
The soil for the nitrification measurements was amended with powdered lucerne meal as a source of organic nitrogen before application of the test item. The lucerne meal had contents of 41.9 % carbon and 2.65 % nitrogen resulting in a C:N ratio of approx. 15.8:1 and was added to the soil in amounts of approx. 5 g/kg dry soil.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Nitrification was determined at DAT 0 and 28
VEHICLE CONTROL PERFORMED: yes - Nominal and measured concentrations:
- Nominal: 62.5, 125, 250, 500 and 1000 mg/kg dry soil
- Reference substance (positive control):
- yes
- Remarks:
- Nitrapyrin PESTANAL (CAS 1929-82-4)
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Details on results:
- The daily soil nitrate formation rate was calculated from the nitrate concentrations at DAT 0 and 28 (Table 5). The daily nitrate formation rate in the control and solvent control was -0.23 and -0.84 mg/kg dry soil/day, respectively, and differed statistically significantly from each other (Student t-test, two-sided, α = 0.05). This difference was considered to be the consequence of calculations based on different starting nitrate concentrations at Day 0 (see section 6.2.3). During the 28 days of the study, the soil microflora in the control and solvent control had sufficient time to equilibrate and produced comparable values of nitrate concentration at DAT 28 (see section 6.2.4). Since the solvent control and the test item treatments were treated with the same solvent, the solvent control was used for further statistical comparisons with the reference item treatment as well as with the test item treatments.
In the test item treatments, mean nitrate formation rate was in the range of -0.92 to 1.38 mg nitrate/kg dry soil/day without a concentration-effect relationship and was not statistically significantly smaller compared to the solvent control (Dunnett t-test, one-sided smaller, α = 0.05). A decrease of soil nitrate concentration between Day 0 and 28 can occur when the microbial community of nitrifiers and denitrifiers change during the course of the study and was found in this study for the majority of the treatments. This decrease was not considered to be caused by the test item since no concentration-effect relationship on nitrate formation rate was observed. - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
The mean nitrate formation rate at DAT 28 in the reference item treatment was 94 % lower than in the solvent control and differed statistically significantly from the solvent control (Student t-test, one-sided smaller, α = 0.05; due to the outlier test after Hampel, replicate 3 of the reference item treatment was excluded). The statistically significantly decreased nitrate formation rate in the reference item treatment was the expected effect of the reference item in order to demonstrate that the used test system was suitable to detect potential effects of the test item on the nitrification potential of the exposed soil microflora. - Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC for nitrate concentration and formation were >= 1000 mg/kg dry soil.
- Executive summary:
In a valid, reliable and conclusive study according to OECD guideline 216 (2000), soil microorganisms were exposed to the test item in natural soil for 28 days. The NOEC and LOEC for nitrate concentration and nitrate formation at Day 28 were ≥ 1000 and > 1000 mg/kg dry soil, respectively.
Reference
Results nitrate concentration
Treatment | Incu-bation time [days] | Nitrate concentration [mg NO3-/ kg dry soil] | Stat. | ||||||
Replicates | % deviation from control | ||||||||
a | b | c | Mean | SD | CV | ||||
Control | 0 | 56,5 | 55,8 | 56,0 | 56,1 | 0,4 | 1 | -21 | s.a |
28 | 49,3 | 48,5 | 51,5 | 49,8 | 1,6 | 3 | 5 | n.s.a | |
Solvent Control Aceton |
0 | 71,3 | 71,0 | 70,0 | 70,8 | 0,7 | 1 | 0 | - |
28 | 45,5 | 47,5 | 49,0 | 47,3 | 1,8 | 4 | 0 | - | |
Neo Heliopan MBC: 62.5 mg/kg dry soil |
0 | 62,3 | 53,0 | 53,5 | 56,3 | 5,2 | 9 | -20 | n.s. |
28 | 93,5 | 92,3 | 98,8 | 94,8 | 3,4 | 4 | 100 | n.s. | |
Neo Heliopan MBC: 125 mg/kg dry soil |
0 | 55,0 | 50,3 | 67,3 | 57,5 | 8,8 | 15 | -19 | n.s. |
28 | 38,5 | 24,3 | 32,8 | 31,8 | 7,2 | 23 | -33 | s. | |
Neo Heliopan MBC: 250 mg/kg dry soil |
0 | 49,5 | 47,5 | 47,8 | 48,3 | 1,1 | 2 | -32 | s. |
28 | 67,3 | 64,5 | 60,5 | 64,1 | 3,4 | 5 | 35 | n.s. | |
Neo Heliopan MBC: 500 mg/kg dry soil |
0 | 87,0 | 87,0 | 93,3 | 89,1 | 3,6 | 4 | 26 | s. |
28 | 70,3 | 67,0 | 80,5 | 72,6 | 7,0 | 10 | 53 | n.s. | |
Neo Heliopan MBC: 1000 mg/kg dry soil |
0 | 66,8 | 91,3 | 71,8 | 76,6 | 12,9 | 17 | 8 | n.s. |
28 | 64,3 | 78,5 | 85,5 | 76,1 | 10,8 | 14 | 61 | n.s. | |
6.7 mg Nitrapyrin/kg dry soila | 0 | 80,5 | 97,0 | 72,0 | 88,8 | 11,7 | 13 | 25 | n.s.b |
28 | 33,0 | 53,8 | 57,8 | 43,4 | 14,7 | 34 | -8 | n.s.c |
Results nitrate formation rate
Treatment | Incu-bation time [days] | Nitrate formation rate [mg NO3-/ kg dry soil/day] | Stat. | ||||||
Replicates | % deviation from control | ||||||||
a | b | c | Mean | SD | CV | ||||
Control | 28 | -0,26 | -0,26 | -0,16 | -0,23 | 0,06 | -25 | -73 | s.a |
Solvent Control Aceton |
28 | -0,92 | -0,84 | -0,75 | -0,84 | 0,08 | -10 | 0 | - |
Neo Heliopan MBC: 62.5 mg/kg dry soil |
28 | 1,12 | 1,40 | 1,62 | 1,38 | 0,25 | 18 | -265 | n.s. |
Neo Heliopan MBC: 125 mg/kg dry soil |
28 | -0,59 | -0,93 | -1,23 | -0,92 | 0,32 | -35 | 10 | n.s. |
Neo Heliopan MBC: 250 mg/kg dry soil |
28 | 0,63 | 0,61 | 0,46 | 0,57 | 0,10 | 17 | -168 | n.s. |
Neo Heliopan MBC: 500 mg/kg dry soil |
28 | -0,60 | -0,71 | -0,46 | -0,59 | 0,13 | -22 | -30 | n.s. |
Neo Heliopan MBC: 1000 mg/kg dry soil |
28 | -0,09 | -0,46 | 0,49 | -0,02 | 0,48 | -2673 | -98 | n.s. |
6.7 mg Nitrapyrin/kg dry soila | 28 | -1,70 | -1,54 | -0,51 | -1,62 | 0,11 | -7 | 94 | s.b |
Description of key information
The NOEC (28 days) for nitrate concentration and formation was found to be >= 1000 mg/kg dry soil in an OECD 216 test with soil microorganisms.
Key value for chemical safety assessment
- Long-term EC10 or NOEC for soil microorganisms:
- 1 000 mg/kg soil dw
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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