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Diss Factsheets
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EC number: 701-394-3 | CAS number: 1782069-81-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Sediment toxicity
Administrative data
Link to relevant study record(s)
- Endpoint:
- sediment toxicity: long-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 225 (Sediment-Water Lumbriculus Toxicity Test Using Spiked Sediment)
- Version / remarks:
- 2006
- Deviations:
- not specified
- GLP compliance:
- not specified
- Remarks:
- Published study from a peer-reviewed journal.
- Analytical monitoring:
- yes
- Details on sampling:
- SEDIMENT
- Sampling interval: Sediment samples were taken at the end of the test (28 days) - Vehicle:
- yes
- Remarks:
- ethyl acetate
- Details on sediment and application:
- PREPARATION OF SPIKED SEDIMENT
- Details of spiking: The whole amount of sediment was treated with the test item.
- Equilibration time: 7 days
- Equilibration conditions: Test conditions
- Controls: Unspiked sediment
- Chemical name of vehicle: Ethyl acetate
- Concentration of vehicle in test medium (stock solution and final test solution): Not reported
- Evaporation of vehicle before use: Yes, for 24 hours - Test organisms (species):
- Lumbriculus variegatus
- Details on test organisms:
- TEST ORGANISM
- Source: Lab culture of ECT Oekotoxikologie GmbH (Flörsheim, Germany)
- Breeding conditions: Worms were kept in continuously aerated 5 L aquaria filled with reconstituted water (pH-value: 7.9 to 8.4, conductivity: 540 μS/cm) and a sediment layer of 1 cm depth, containing pure quartz sand with a grain size between 0.1 and 0.4 mm. The culture was maintained at 20 ± 1 °C and the light dark rhythm was adjusted to 16:8 h.
- Age of animals at beginning of exposure: Not reported, worms of the same developmental status were used for the test.
- Food type: Tetramin
- Amount: ad libitum
- Frequency: Once a week - Study type:
- laboratory study
- Test type:
- static
- Water media type:
- freshwater
- Type of sediment:
- artificial sediment
- Limit test:
- no
- Duration:
- 28 d
- Exposure phase:
- total exposure duration
- Test temperature:
- 20 ± 1 °C
- pH:
- 7.9 to 8.4
- Conductivity:
- 540 μS/cm
- Nominal and measured concentrations:
- 0.08, 0.4, 2, 10 and 50 mg/kg dw sediment
< 0.05, 0.21, 1.47, 6.18, 51.5 mg/kg sediment dw (measured at day 28) - Details on test conditions:
- TEST SYSTEM
- Test container (material, size): 500 mL glass beakers (8 cm diameter), covered with a plastic cap.
- Sediment volume: Each beaker was filled with 40 g dw spiked artificial sediment.
- Overlying water volume: 200 mL
- Depth of sediment and overlying water: Sediment layer 1 cm deep, overlying water depth not reported.
- Aeration: yes
- Aeration frequency and intensity: Continuously aerated
EXPOSURE REGIME
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control / vehicle control: 4
OVERLYING WATER CHARACTERISTCS
- Type of water: Reconstituted water
CHARACTERIZATION OF ARTIFICIAL SEDIMENT
- Particle size distribution
- % sand: pure quartz sand with a grain size between 0.1 and 0.4 mm
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark
EFFECT PARAMETERS MEASURED: At the end of the test, the number and weight of the worms were recorded as endpoints. Once in a week, physical and chemical parameters of the overlying water such as temperature, pH-value, conductivity, and
nitrite and ammonium content were measured and recorded.
VEHICLE CONTROL PERFORMED: yes - Reference substance (positive control):
- no
- Key result
- Duration:
- 28 d
- Dose descriptor:
- EC10
- Effect conc.:
- >= 51.5 mg/kg sediment dw
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks:
- and weight
- Remarks on result:
- not determinable
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- >= 51.5 mg/kg sediment dw
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks:
- and weight
- Remarks on result:
- not determinable
- Details on results:
- - Mortality of test animals at end of exposure period: Due to the fast degradation of dead worms in the sediment, mortality could not be measured.
- Reported statistics and error estimates:
- All data were tested for normality by the Kolmogorov-Smirnov test. As the data were normally distributed, significant differences compared to the solvent control were calculated using a one-way analysis of variance (ANOVA) followed by Dunn’s post hoc test.
- Validity criteria fulfilled:
- yes
- Remarks:
- The observed factor of reproduction in the control groups with a value of 3.98 within 28 days (must be ≥ 1.80). At the end of the test, the pH ranged between 7.4 and 8.6 and dissolved oxygen level was always above 60% of air saturation volume.
- Conclusions:
- There were no significant effects on reproduction or biomass up to 51.5 mg/kg sediment dw, the highest concentration tested.
- Executive summary:
The chronic toxicity of 4-methylbenzylidene camphor to Lumbriculus variegatus was determined in a 28 -day test under static conditions. Test organisms were exposed to five nominal concentrations of 0.08, 0.4, 2, 10 and 50 mg/kg sediment dry weight, alongside a control and solvent control. Four replicates were used for each treatment group and control, with each replicate containing 10 test organisms. The number and weight of the worms were determined at the end of the test. Test item concentrations were analysed on day 28 and measured concentrations were used for the reporting of results. There were no concentration dependent, significant effects on reproduction or biomass up to 51.5 mg/kg sediment dw, the highest concentration tested. This study is considered to be reliable with restrictions (Klimisch 2) as it was conducted according to OECD Guideline 225, with minor limitations in the reporting.
Reference
Table 1. Nominal and measured test concentrations at the end of the test
Nominal concentration on day 0 (mg/kg sediment dw) | Measured concentration on day 28 (mg/kg sediment dw) | % Recovery on day 28 |
Control | not detectable | - |
Solvent control | not detectable | - |
0.08 | < 0.05 | - |
0.40 | 0.21 | 55.1 |
2.00 | 1.47 | 76.9 |
10.0 | 6.18 | 64.9 |
50.0 | 51.5 | 108 |
Table 2. Reproduction and weight of Lumbriculus variegatus exposed to the test item in a 28-day sediment test (mean ± standard deviation; n = 4)
Measured concentration (mg/kg sediment dw) | Reproduction (number of worms per test vessel) | Dry weight (mg/worm) | Total biomass (mg) |
Control | 39.8 ± 12.7 | 0.59 ± 0.38 | 41.0 ± 19.0 |
Solvent control | 34.3 ± 2.50 | 0.92 ± 0.23 | 39.5 ± 1.22 |
0.06 | 25.0 ± 4.40 | 1.16 ± 0.23 | 29.2 ± 7.92 |
0.21 | 23.5 ± 4.20 | 1.16 ± 0.27 | 27.1 ± 7.51 |
1.47 | 24.3 ± 5.12 | 1.33 ± 0.15 | 32.3 ± 8.15 |
6.18 | 22.3 ± 2.63* | 1.19 ± 0.37 | 26.0 ± 6.89 |
51.5 | 23.5 ± 1.92 | 1.24 ± 0.24 | 29.2 ± 7.09 |
Description of key information
There were no significant effects on reproduction or biomass of Lumbricus variegatus up to 51.5 mg/kg sediment dw, the highest concentration tested, after 28 -day exposure to the test item.
Key value for chemical safety assessment
- EC10, LC10 or NOEC for freshwater sediment:
- 51.5 mg/kg sediment dw
Additional information
The chronic toxicity of 3-(4'-methylbenzylidene)-camphor to Lumbricus variegatus was determined in a 28 -day test under static conditions. Test organisms were exposed to five nominal concentrations of 0.08, 0.4, 2, 10 and 50 mg/kg sediment dry weight, alongside a control and solvent control. Four replicates were used for each treatment group and control, with each replicate containing 10 test organisms. The number and weight of the worms were determined at the end of the test. Test item concentrations were analysed on day 28 and measured concentrations were used for the reporting of results. There were no significant effects on reproduction or biomass up to 51.5 mg/kg sediment dw, the highest concentration tested. This study is considered to be reliable with restrictions (Klimisch 2) as it was conducted according to OECD Guideline 225, with minor limitations in the reporting.
Furthermore the chronic toxicity of the test substance to hte gastropod mollusc Potamopyrgus antiopodarum was investigated in a 56 -day sediment exposure test under static conditions. Due to methodological deficiencies this study was assessed as not reliable (RL=3) and therefore disregarded for the chemical safety assessment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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