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EC number: 257-473-3 | CAS number: 51851-37-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997-04-22 to 1997-04-24
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0(Control), 6.7, 13.4, 33.4 and 66.8 mg/L and test stock solution.
- Sampling method: A series of concentrations of the test substance in distilled, deionised water were prepared to assess their stability. Their carbon content was measured initially and then again after 24, 48 and 72 hours. The concentration of the freshly prepared stock solution (in articial fresh water) used to prepare the test media was also determined. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A 1000 mg/L stock solution was prepared by adding the test substance to artificial fresh water and then stirring for 18 hours followed by filtration.
- Controls: Artificial fresh water - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain: Clone 5
- Source: Own laboratory culture
- Age at study initiation (mean and range, SD): <24 hours
- Method of breeding: Parthenogenic reproduction
- Feeding during test: none
ACCLIMATION
- Acclimation conditions: same as test
- Type and amount of food: Scenedesmus subspicatus is added to the cultures in amounts such that it is all consumed prior to feeding again the next day.
- Feeding frequency: daily - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 250 mg/L as CaCO3
- Test temperature:
- 20+/-1ºC
- pH:
- 7.9-8.0
- Dissolved oxygen:
- 8.2-8.6 mg/L
- Salinity:
- not applicable
- Nominal and measured concentrations:
- Nominal loading rate: 0 (Control) and 1000 mg/L
Dissolved organic carbon (DOC) concentration of stock solution: 9 mg/L
Dissolved organic carbon (DOC) concentration of test media at start of test: 10 mg/L.
Results of the analysis of media prepared for assessing the stability of the test solutions showed that for nominal concentrations in the range 6.7 to 66.8 mg/L the concentration after 72 hours was at least 80% of that in the freshly prepared media.
The test results are interpreted with respect to the measured DOC concentration of the stock solution (9 mg/L) and the initial loading rate of the test substance used in the preparation of the stock solution (1000 mg/L). - Details on test conditions:
- TEST SYSTEM
- Test vessel: Rimmed test tubes
- Type: open
- Material, size, headspace, fill volume: glass, 10 mL with 10 mL of test medium
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 2 mL/test organism
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Artificial fresh water
- Ca/mg ratio: 4:1
- Culture medium different from test medium: yes (Elendt M4 medium used for culture)
- Intervals of water quality measurement: Start and end of test
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: Continuous darkness
EFFECT PARAMETERS MEASURED: Mobility after 24 and 48 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable
- Justification for using less concentrations than requested by guideline: not reported - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: hydrolysis products
- Basis for effect:
- mobility
- Remarks on result:
- other: based on measured DOC concentration
- Duration:
- 48 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: hydrolysis products
- Basis for effect:
- mobility
- Remarks on result:
- other: based on measured DOC concentration
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- but exposure is to hydrolysis products
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: 0
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- 24-h EC50: in the range 1-2 mg/L, which is within the normal range for the laboratory culture. - Reported statistics and error estimates:
- Not applicable - no effects observed at the only test concentration
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 48-hour EC50 value of >9.0 mg/L and a NOECof ≥9.0 mg/L have been determined for the effects of the test substance on mobility of Daphnia magna based on mean measured initial Dissolved Organic Carbon (DOC) concentration. The test medium was prepared at an initial loading rate of 1000 mg/L of the substance and was subsequently filtered before introducing the test organisms. The test substance hydrolyses in water and it is likely that the test organisms were primarily exposed to the hydrolysis products of the substance.
Reference
Table 1. Results of analysis of test media and stability test media
Nominal test substance concentration (mg/L) | Measured concentration at start (mg/L) | Measured concentration after 24 h (mg/L) | Measured concentration after 48 h (mg/L) | Measured concentration after 72 h (mg/L) | Mean measured concentration (mg/L) |
66.8 | 66.8 | 54.8 | 56.3 | 53.7 | 57.9 |
33.4 | 31.2 | 28.0 | 27.4 | 26.8 | 28.4 |
13.4 | 13.3 | 11.3 | 13.1 | 12.2 | 12.5 |
6.7 | 6.8 | 5.1 | 5.2 | 7.0 | 6.0 |
9.0 (Test medium stock solution. Initial nominal loading rate of 1000 mg/L) | 10 | - | - | - | - |
No toxic effects were observed in the Control or the only test loading rate/concentration.
Description of key information
Short-term toxicity to aquatic invertebrates: 48-hour EC50 >9.0 mg/l (measured) (highest concentration tested) (EU Method C.2 (Acute Toxicity for Daphnia)).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- > 9 mg/L
Additional information
A 48-hour EC50 value of >9.0 mg/l (measured) (highest concentration tested) have been determined for the effects of [2-(perfluorohexyl)ethyl]triethoxysilane (CAS 51851-37-7, EC 257-473-3) on mobility of Daphnia magna based on mean measured initial Dissolved Organic Carbon (DOC) concentration. The test medium was prepared at an initial loading rate of 1000 mg/l of the substance and was subsequently filtered before introducing the test organisms.
As the extremely low water solubility of the test substance will limit hydrolysis, it is likely that the test organisms were exposed to parent substance.
Long-term aquatic invertebrate testing of the parent substance has not been considered feasible due to the very low water solubility of the substance (6.4E-06 mg/l) and very high log Kow(7.2). These values indicate that maintaining stable aqueous test solutions will be extremely difficult. Furthermore, once dissolved, the substance will have the tendency to adsorb onto the glassware under experimental conditions and onto dissolved organic matter in the natural environment.
Refer to IUCLID Section 6.1 and Section 7.1 of the CSR for discussion of the surface active properties of the parent substance, silanol hydrolysis product and condensation products and further discussion of the hydrolysis rates of the parent substance.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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