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EC number: 221-573-5 | CAS number: 3147-75-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992-08-25 to 1993-01-20
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Non-GLP-guideline study. Deviations to the updated OECD Guideline (adopted 2006): The coefficient of analysis of the daily growth rate in the control replicates and the coefficient of analysis of the average specific growth rates in the control replicates were not reported. No analytical confirmation.
- Qualifier:
- according to guideline
- Guideline:
- other: 87/302/EEC page 89-94 Algal growth inhibition test
- Deviations:
- no
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 2.0 g of test substance and and 18 g of a 0.4% vehicle solution (Akropal) were mixed together by means of marble-mill for about 24 hours. 1 ml of this blend were mixed and made up to 1000 ml with water achieving a concentration of 100 mg/L
- Controls: Blank control: water, vehicle control: 3.6 mg Akropal/L
- Chemical name of vehicle: alcyl-phenol-polyglycol-ether (Akropal)
- Evidence of undissolved material (e.g. precipitate, surface film, etc): at concentrations of 3.7, 11, 33 and 100 mg/L small parts of the test substance were observed on the surface of the test water .
- Concentration of vehicle in test medium: 0.4 % Akropal (stock solution) - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: Green algae (Scenedesmus subspicatus)
- Source (laboratory, culture collection): Collection of algae cultures, Pflanzenphysiologisches Institut, Universitiy, Nikolausberger Weg 18, 3400 Göttingen, Germany
- Method of cultivation: according to guideline
ACCLIMATION
- Acclimation period: preculture 3 days under test conditions
- Culturing media and conditions (same as test or not): preculture under test conditions
- Any deformed or abnormal cells observed: none reported - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Test temperature:
- 22 ± 2°C
- pH:
- 7.9 - 9.8
- Nominal and measured concentrations:
- Nominal: 1.23, 3.7, 11, 33, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flask
- Type (delete if not applicable): stoppered with aluminium caps, on Lab-shaker
- Material, size, headspace, fill volume: 100 ml Erlenmeyer flasks filled with 50 ml test solution
- Initial cells density: 10500 cells/ml
- Control end cells density: mean cell density after 72 h per ml: 209.8
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes, according to cited guideline
TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: temperature was continously measured, pH was measured at 0h and 72h
OTHER TEST CONDITIONS
- Photoperiod: continously
- Light intensity and quality: Flourescent light, approx. 8000 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: "TOA" cell counter
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3 - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control: yes
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: at testconcentrations 3.7, 11, 33 and 100 mg/l small parts of the test substance were swimming on the surface of the test water after 72 hours of exposure
- Effect concentrations exceeding solubility of substance in test medium: yes - Reported statistics and error estimates:
- The EbC50 values were calculated according to the maximum likelihood method, robit model (Mc Cullagh, P., Nelder, J.A., 1983: Generalized linear models, Chapman & Hall, London)
Reference
Description of key information
With high probability not toxic to aquatic algae and cyanobacteria. No toxic effects occur in the range of water solubility.
Key value for chemical safety assessment
Additional information
In a 72 hour acute toxicity study, the cultures of Scenedesmus subspicatus were exposed to the test item at nominal concentrations of 0, 1.23, 3.77, 11, 33 and 100 mg/L under static conditions in accordance with the EEC guideline 302/87 (1987). The EbC50 values after 72 hours of exposure based on cell number were > 100 mg/L and the NOEbC values after 72 hours of exposure based on cell number were 100 mg/L.
This toxicity study is classified as acceptable and satisfies the guideline requirements for OECD 201 toxicity study.
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