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EC number: 201-240-0 | CAS number: 79-97-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
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- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
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- Endpoint summary
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 2010
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Remarks:
- Stability of test substance was measured
- Details on sampling:
- The test substance was stored as follows: room temperature, dark, air-tight in a desiccator.
Sampled before and after exposure - Vehicle:
- no
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected:
Konan-Chubu Water Reclamation Plant
- Preparation of inoculum for exposure: MLSS was measured and adjusted to 3000 mg/L by adding ultra pure water on the day of collection. In addition, the synthetic sewage feed was added to the sludge (50mL synthetic sewage feed/1L sludge) and was stored until use for the exposure. The pH was checked and adjusted by adding NaOH to pH 7.5 -/= 0.5 on the day of exposure.
- Storage conditions: 20 +/- 2°C
- Mixed liquor suspended solid concentration (MLSS): 3180 mg/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- Not applicable
- Test temperature:
- 20 +/- 2°C
- Nominal and measured concentrations:
- 1, 5.6, 32, 180, 1000 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1000 mL glass beakers
- Aeration: 0.5 to 1L/min
- No. of vessels per concentration (replicates): 3 (main phase)
- No. of vessels per control (replicates): 6 (main phase)
- Nutrients provided for bacteria: Syntheic sewage feed
- Nitrification inhibitor used: N-allylthiourea
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Ultra pure water
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : 3 hour respiration inhibition (based on oxygen consumption).
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 5.62
- Range finding study
- Test concentrations: 1,10, 100 and 1000 mg/L (nominal)
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol (DCP)
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Basis for effect:
- inhibition of nitrification rate
- Remarks on result:
- not determinable
- Remarks:
- % of inhibition fluctuated largely. Total respiration was greatly attributed to inhibition of hetrotrophic respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 5.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Temperature was maintained at 20.3°C
See tables in Any other information on results incl. tables. - Results with reference substance (positive control):
- EC50 of DPC = 7.79 mg/L (Total respiration), 9.3 mg/L (Heterotrophic respiration) and 0.137 mg/L (nitrification respiration). Validity criteria met for reference substance.
- Reported statistics and error estimates:
- NOEC value was determined by an analysis of variance (ANOVA), Williams test. The significant level for test was set a p=0.05
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 3h EC50 value was calculated to be >1000 mg/L for total respiration inhibition. The NOEC was estimated to be 5.6 mg/L for total respiration inhibition.
Reference
Table 1: pH values
Concentration (mg/L) |
Type of respiration |
Total respiration |
Type of respiration |
Heterotrophic respiration |
||
No. |
pH |
No. |
pH |
|||
1* |
2* |
1* |
2* |
|||
Control |
FB-1 |
7.4 |
7.7 |
FBH-1 |
7.4 |
7.8 |
FB-2 |
- |
7.7 |
FBH-2 |
- |
7.8 |
|
FB-3 |
7.7 |
FBH-3 |
7.8 |
|||
FB-4 |
7.7 |
FBH-4 |
7.8 |
|||
FB-5 |
7.7 |
FBH-5 |
7.8 |
|||
FB-6 |
7.7 |
FBH-6 |
7.8 |
|||
1.0 |
FT1-1 |
7.4 |
7.7 |
FTH1-1 |
7.4 |
7.9 |
FT1-2 |
- |
7.6 |
FTH1-2 |
- |
7.8 |
|
FT1-3 |
7.7 |
FTH1-3 |
7.8 |
|||
5.6 |
FT2-1 |
7.4 |
7.7 |
FTH2-1 |
7.4 |
7.7 |
FT2-2 |
- |
7.6 |
FTH2-2 |
- |
7.8 |
|
FT2-3 |
7.6 |
FTH2-3 |
7.8 |
|||
32 |
FT3-1 |
7.4 |
7.6 |
FTH3-1 |
7.4 |
7.8 |
FT3-2 |
- |
7.6 |
FTH3-2 |
- |
7.7 |
|
FT3-3 |
7.6 |
FTH3-3 |
7.7 |
|||
180 |
FT4-1 |
7.4 |
7.5 |
FTH4-1 |
7.4 |
7.9 |
FT4-2 |
- |
7.5 |
FTH4-2 |
- |
7.7 |
|
FT4-3 |
7.6 |
FTH4-3 |
7.7 |
|||
1000 |
FT5-1 |
7.4 |
7.6 |
FTH5-1 |
7.4 |
7.8 |
FT5-2 |
- |
7.5 |
FTH5-2 |
- |
7.8 |
|
FT5-3 |
7.5 |
FTH5-3 |
7.7 |
1* Before adding activated sludge
2* At the end of exposure period
Table 2: Oxygen consumption rates of blank controls
Type of respiration |
No. |
Concentration (mg/L) |
Time (min) |
DO (mg/L) |
R (mgO2/L/hr) |
Average |
SD |
CV (%) |
||
(mgO2/L/hr) |
||||||||||
Total respiration |
FB-1 |
- |
0.75 |
Q1 |
3.96 |
RTB-1 |
46.8 |
45.2 |
1.1 |
2.4 |
3.25 |
Q2 |
2.01 |
||||||||
FB-2 |
0.75 |
Q1 |
3.41 |
RTB-2 |
44.9 |
|||||
2.58 |
Q2 |
2.04 |
||||||||
FB-3 |
0.75 |
Q1 |
3.69 |
RTB-3 |
45.3 |
|||||
2.92 |
Q2 |
2.05 |
||||||||
FB-4 |
0.75 |
Q1 |
2.96 |
RTB-4 |
43.3 |
|||||
2.08 |
Q2 |
2.00 |
||||||||
FB-5 |
0.75 |
Q1 |
3.22 |
RTB-5 |
45.2 |
|||||
2.33 |
Q2 |
2.03 |
||||||||
FB-6 |
0.75 |
Q1 |
4.27 |
RTB-6 |
45.6 |
|||||
3.67 |
Q2 |
2.05 |
||||||||
Heterotrophic respiration |
FBH-1 |
1.00 |
Q1 |
4.99 |
RHB-1 |
36.6 |
40.1 |
- |
- |
|
5.83 |
Q2 |
2.04 |
||||||||
FBH-2 |
1.00 |
Q1 |
5.14 |
RHB-2 |
37.6 |
|||||
6.00 |
Q2 |
2.01 |
||||||||
FBH-3 |
1.00 |
Q1 |
4.71 |
RHB-3 |
40.9 |
|||||
4.92 |
Q2 |
2.04 |
||||||||
FBH-4 |
1.00 |
Q1 |
5.53 |
RHB-4 |
40.7 |
|||||
6.17 |
Q2 |
2.02 |
||||||||
FBH-5 |
1.00 |
Q1 |
4.41 |
RHB-5 |
41.3 |
|||||
4.50 |
Q2 |
2.00 |
||||||||
FBH-6 |
1.00 |
Q1 |
4.20 |
RHB-6 |
43.4 |
|||||
4.00 |
Q2 |
2.03 |
||||||||
Nitrification respiration |
- |
|
|
|
RNB-1 |
6.7 |
5.1 |
- |
- |
|
RNB-2 |
4.8 |
|||||||||
RNB-3 |
5.2 |
|||||||||
RNB-4 |
3.2 |
|||||||||
RNB-5 |
5.1 |
|||||||||
RNB-6 |
5.5 |
Equation:
R=(Q1-Q2)/Δtx60
RNB=RTB-RHB (Average)
Table 3: Oxygen consumption rates, percent inhibitions of test mixtures (Total respiration)
Type of respiration |
No. |
Concentration (mg/L) |
Time (min) |
DO (mg/L) |
R (mgO2/L/hr) |
Average (mgO2/L/hr) |
Inhibition |
Average (%) |
|||
Total respiration
|
FT1-1 |
1.0 |
0.75 |
Q1 |
4.06 |
RTT1-1 |
48.5 |
46.2 |
ITT1 |
-7.3 |
-2.3 |
3.25 |
Q2 |
2.04 |
|||||||||
FT1-2 |
0.75 |
Q1 |
3.63 |
RTT1-2 |
45.3 |
-0.2 |
|||||
2.83 |
Q2 |
2.06 |
|||||||||
FT1-3 |
0.75 |
Q1 |
3.28 |
RTT1-3 |
44.9 |
0.7 |
|||||
2.42 |
Q2 |
2.03 |
|||||||||
FT2-1 |
5.6 |
0.75 |
Q1 |
2.02 |
RTT2-1 |
45.6 |
44.5 |
ITT2 |
-0.9 |
1.5 |
|
2.58 |
Q2 |
0.63 |
|||||||||
FT2-2 |
0.75 |
Q1 |
3.74 |
RTT2-2 |
44.3 |
2.0 |
|||||
3.08 |
Q2 |
2.02 |
|||||||||
FT2-3 |
0.75 |
Q1 |
3.17 |
RTT2-3 |
43.7 |
3.3 |
|||||
2.33 |
Q2 |
2.02 |
|||||||||
FT3-1 |
32 |
0.75 |
Q1 |
2.71 |
RTT3-1 |
36.4 |
40.8 |
ITT3 |
19.5 |
9.8 |
|
3.67 |
Q2 |
0.94 |
|||||||||
FT3-2 |
0.75 |
Q1 |
1.88 |
RTT3-2 |
41.3 |
8.6 |
|||||
2.42 |
Q2 |
0.73 |
|||||||||
FT3-3 |
0.75 |
Q1 |
2.91 |
RTT3-3 |
44.6 |
1.3 |
|||||
1.92 |
Q2 |
2.04 |
|||||||||
FT4-1 |
180 |
0.75 |
Q1 |
3.16 |
RTT4-1 |
34.8 |
31.1 |
ITT4 |
23.0 |
31.1 |
|
2.75 |
Q2 |
2.00 |
|||||||||
FT4-2 |
0.75 |
Q1 |
2.91 |
RTT4 -2 |
28.1 |
37.8 |
|||||
2.67 |
Q2 |
2.01 |
|||||||||
FT4-3 |
0.75 |
Q1 |
3.69 |
RTT4-3 |
30.5 |
32.5 |
|||||
4.67 |
Q2 |
2.04 |
|||||||||
FT5-1 |
1000 |
0.75 |
Q1 |
1.97 |
RTT-5-1 |
32.2 |
31.0 |
ITT5 |
28.8 |
31.5 |
|
3.08 |
Q2 |
0.72 |
|||||||||
FT5-2 |
0.75 |
Q1 |
2.67 |
RTT5-2 |
33.8 |
25.2 |
|||||
1.92 |
Q2 |
2.01 |
|||||||||
FT5-3 |
0.75 |
Q1 |
3.77 |
RTT5-3 |
26.9 |
40.5 |
|||||
4.67 |
Q2 |
2.01 |
Equation:
R=(Q1-Q2)/Δtx60
ITT={1-RTT/RTB (Average)} x 100
Table 4: Oxygen consumption rates, percent inhibitions of test mixtures (heterotropic respiration)
Type of respiration |
No. |
Concentration (mg/L) |
Time (min) |
DO (mg/L) |
R (mgO2/L/hr) |
Average (mgO2/L/hr) |
Inhibition |
Average (%) |
|||
Heterotrophic respiration
|
FTH1-1 |
1.0 |
1.00 |
Q1 |
5.67 |
RHT1-1 |
40.2 |
39.3 |
IHT1 |
-0.2 |
2.1 |
6.42 |
Q2 |
2.04 |
|||||||||
FTH1-2 |
1.00 |
Q1 |
5.02 |
RHT1-2 |
38.0 |
5.2 |
|||||
5.75 |
Q2 |
2.01 |
|||||||||
FTH1-3 |
1.00 |
Q1 |
4.76 |
RHT1-3 |
39.6 |
1.2 |
|||||
5.17 |
Q2 |
2.01 |
|||||||||
FTH2-1 |
5.6 |
1.00 |
Q1 |
4.26 |
RHT2-1 |
38.1 |
38.2 |
IHT2 |
5.0 |
4.8 |
|
4.50 |
Q2 |
2.04 |
|||||||||
FTH2-2 |
1.00 |
Q1 |
5.76 |
RHT2-2 |
37.7 |
6.0 |
|||||
6.92 |
Q2 |
2.04 |
|||||||||
FTH2-3 |
1.00 |
Q1 |
4.51 |
RHT2-3 |
38.7 |
3.5 |
|||||
4.83 |
Q2 |
2.04 |
|||||||||
FTH3-1 |
32 |
1.00 |
Q1 |
2.20 |
RHT3-1 |
28.8 |
30.9 |
IHT3 |
28.2 |
23.0 |
|
3.50 |
Q2 |
1.00 |
|||||||||
FTH3-2 |
1.00 |
Q1 |
4.84 |
RHT3-2 |
31.1 |
22.4 |
|||||
6.42 |
Q2 |
2.03 |
|||||||||
FTH3-3 |
1.00 |
Q1 |
4.65 |
RHT3-3 |
32.7 |
18.5 |
|||||
5.83 |
Q2 |
2.02 |
|||||||||
FTH4-1 |
180 |
6.13 |
Q1 |
6.13 |
RHT4-1 |
23.9 |
26.2 |
IHT4 |
40.4 |
34.7 |
|
2.54 |
Q2 |
2.54 |
|||||||||
FTH4-2 |
5.19 |
Q1 |
5.19 |
RHT4-2 |
26.4 |
34.2 |
|||||
2.00 |
Q2 |
2.00 |
|||||||||
FTH4-3 |
4.75 |
Q1 |
4.75 |
RHT4-3 |
28.3 |
29.4 |
|||||
2.00 |
Q2 |
2.00 |
|||||||||
FTH5-1 |
1000 |
5.22 |
Q1 |
5.22 |
RHT-5-1 |
36.0 |
28.6 |
IHT5 |
10.2 |
28.7 |
|
2.07 |
Q2 |
2.07 |
|||||||||
FTH5-2 |
5.51 |
Q1 |
55.1 |
RHT5-2 |
25.0 |
37.7 |
|||||
2.00 |
Q2 |
2.00 |
|||||||||
FTH5-3 |
5.42 |
Q1 |
5.42 |
RHT5-3 |
24.8 |
38.2 |
|||||
2.01 |
Q2 |
2.01 |
Equation:
R=(Q1-Q2)/Δtx60
ITT={1-RHT/RHB (Average)} x 100
Table 5: Oxygen consumption rates, percent inhibitions of test mixtures (nitrification respiration)
Type of respiration |
No. |
Concentration (mg/L) |
R (mgO2/L/hr) |
Average (mgO2/L/hr) |
Inhibition |
Average (%) |
||
Nitrification respiration
|
- |
1.0 |
RNT1-1 |
9.2 |
6.9 |
IHT1 |
-80.4 |
-35.9 |
RNT1-2 |
6.0 |
-17.6 |
||||||
RNT1-3 |
5.6 |
-9.8 |
||||||
- |
5.6 |
RNT2-1 |
7.4 |
6.3 |
IHT2 |
-45.1 |
-24.2 |
|
RNT2-2 |
6.1 |
-19.6 |
||||||
RNT2-3 |
5.5 |
-7.8 |
||||||
- |
32 |
RNT3-1 |
5.5 |
9.9 |
IHT3 |
-7.8 |
-93.4 |
|
RNT3-2 |
10.4 |
-103.9 |
||||||
RNT3-3 |
13.7 |
-168.6 |
||||||
- |
180 |
RNT4-1 |
8.6 |
4.9 |
IHT4 |
-68.6 |
3.3 |
|
RNT4-2 |
1.9 |
62.7 |
||||||
RNT4-3 |
4.3 |
15.7 |
||||||
- |
1000 |
RNT-5-1 |
3.6 |
2.4 |
IHT5 |
29.4 |
53.6 |
|
RNT5-2 |
5.2 |
-2.0 |
Equation:
R=(Q1-Q2)/Δtx60
ITT={1-RNT/RNB (Average)} x 100
Description of key information
OECD guideline, GLP compliant study. Activated sludge was exposed to the test substance for 3 hours during the test. % of respiration inhibition (total, heterotrophic, nitrification) was recorded.
EC50 value of >1000 mg/L and NOEC value of 5.6 mg/L were calculated for total respiration inhibition.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 5.6 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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