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EC number: 201-240-0 | CAS number: 79-97-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22 Nov - 27 Dec 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Version / remarks:
- 17th Jul 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- National GLP Compliance Monitoring Authority, Gov. of India (22 Jul 2016)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Secondary effluent of JRF Sewage Treatment Plant treating predominantly domestic sewage
- Storage conditions: aerobic (aeration) at 22 ± 2 °C
- Storage length: 7 d
- Pretreatment: The secondary effluent was filtered through Whatman No 1 filter paper.
- Concentration of sludge: 2.01 x 10E+04 CFU/mL (bacteria), 2.00 x 10E+02 CFU/mL (fungus)
- Initial cell/biomass concentration: 1 mL/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 5.02 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral medium according to guideline
- Test temperature: 22 ± 2 °C
- Aeration of dilution water: The mineral medium was strongly aerated for 20 min using aerator and allowed to stand for 20 h at 22 ± 2 °C in a horizontal laminar flow under aseptic conditions.
- Continuous darkness: Yes
- Other: For each test, only one batch of high purity water (Type I Milli-Q) and mineral medium was used.
TEST SYSTEM
- Culturing apparatus: BOD incubator (KB-12, LCI-290)
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Aeration of mineral medium (20 min) and secondary effluent (7 d)
- Measuring equipment: Dissolved Oxygen / pH Meter (HQ-40d)
- Test performed in open system: No (Closed Bottle Test)
- Other: 10 test bottles each were prepared for the test suspension, inoculum blanc and the procedure control. 6 bottles were prepared for the toxicity control. The bottles of the respective series will be withdrawn in replicate for dissolved oxygen analysis.
SAMPLING
- Sampling frequency: After 0, 7, 14, 21, and 28 d
- Sampling method: The dissolved oxygen of each sample bottle was measured with a Dissolved Oxygen Meter.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes, 2 replicates
- Procedure control: Yes, 2 replicates
- Toxicity control: Yes, 2 replicates - Reference substance:
- other: Potassium hydrogen phtalate
- Remarks:
- C8H5KO4
- Parameter:
- % degradation (O2 consumption)
- Value:
- 5.99
- Sampling time:
- 28 d
- Results with reference substance:
- The percent degradation of the reference substance (potassium hydrogen phthalate) on Day 7, 14, 21, and 28 was 80.51, 83.23, 85.11, and 85.79, respectively.
- Interpretation of results:
- under test conditions no biodegradation observed
- Remarks:
- For further details on validity criteria please refer to “Any other information on results incl. tables”.
Reference
VALIDITY CRITERIA
The validity criteria of the guideline are summarized below (Table 1).
Table 1: Validity criteria for OECD 301.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%. |
Mean degradation of the test item did not reach 20% within 28 d. Criterion cannot be assessed. |
- |
Percentage degradation of the reference compound has reached the pass levels (60%) by day 14. |
83.23% on Day 14 |
Yes |
Oxygen depletion in the inoculum blank should not exceed 1.5 mg dissolved oxygen/L after 28 days. |
Oxygen depletion in the inoculum blank was 1.31 mg dissolved oxygen/L after 28 d. |
Yes |
The residual concentration of oxygen in the test bottles should not fall below 0.5 mg/L at any time. |
Residual concentrations were 6.42 mg/L. |
Yes |
Toxicity control: toxic, if % degradation < 25% within 14 d. |
The percent degradation by the toxicity control was 22.58%. |
No |
BIOLOGICAL RESULTS
The results are summarized in Table 2.
Table 2. Results - percent degradation
Treatment |
% degradation (based on ThOD) |
|||
7 |
14 |
21 |
28 |
|
Procedure Control |
80.51 |
83.23 |
85.11 |
85.79 |
Test Solution |
0.92 |
1.30 |
1.45 |
5.99 |
Toxicity Control |
21.43 |
22.58 |
- |
- |
Description of key information
OECD guideline, GLP-complaint study. The biodegradability of the substance was assessed using the Closed Bottle Test. 5.99% biodegradation occurred within the 28 day test period.
The test substance is not rapidly biodegradable under the conditions of the test.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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