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Diss Factsheets
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EC number: 215-290-6 | CAS number: 1319-46-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Ciliated protozoan populations were tested in spiked activated sludge mixed liquor, which is relevant for the effects assessment of STPs. Lead concentrations were measured, and relevant endpoint/end parameter are reported. Followed methodology is well described
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 996
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Guideline:
- other: method described in the publication
- Principles of method if other than guideline:
- Ciliated protozoa populations are exposed for 24 hours to lead chloride, using activated sludge mixed liquor (containing bacteria as food source) astest medium
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Lead dichloride
- EC Number:
- 231-845-5
- EC Name:
- Lead dichloride
- Cas Number:
- 7758-95-4
- Details on test material:
- ACS reagent grade
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- 50 mL of activated sludge mixed liquor was filtered (0.45 ug/L)
Test solutions
- Vehicle:
- no
- Details on test solutions:
- 250 mL sample of activated sludge mixed liquor
Test organisms
- Test organisms (species):
- other: Opercularia minima-Opercularia coarctata-Aspidisca lynceus-Vorticella convallaria-Trochilia minuta-Chilodonella uncinata-Euplotes sp.-Vorticella octava
- Details on inoculum:
- Protozoan community living in the activated sludge mixed liquor collected from the aeration basin of the sewage treatment plant in Roncocesi, Reggio Emilia (Italy), treating urban and industrial waste.
The whole sludge microbial community, maintained into the original microbiota, was tested; tests are therefore more similar to field tests than to lab tests.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
Test conditions
- Hardness:
- no data
- Test temperature:
- 20 +/- 2 °C
- pH:
- 7.6 +/- 0.2
- Dissolved oxygen:
- continuous aeration
- Salinity:
- no data
- Nominal and measured concentrations:
- nominal: 150, 200, 300, 400, 500 mg/L
measured after 24h in activated sludge: 1.50, 1.98, 2.79, 3.09, 6.98 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 mL glass flask containing 250 mL activated sludge mixed liquor
- Control: sample of untreated activated sludge mixed liquor
- No additional feeding during the test; normal bacterial population present in the test medium
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : number of surviving organisms was determined by microscopic observations at 100 x magnification.
Non-motile cells on the surface of the flock were regarded as dead
For each concentration the estimate of protozoan numbers was based on enumerations from sub-samples.
For each concentration two subsamples (replicates) were counted
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- other: LC50
- Effect conc.:
- 5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- element
- Basis for effect:
- other: mortality
- Remarks on result:
- other: 95% CL: 3.57 - 10.2 Species: Opercularia minima
- Duration:
- 24 h
- Dose descriptor:
- other: LC50
- Effect conc.:
- 3.28 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- element
- Basis for effect:
- other: mortality
- Remarks on result:
- other: 95%CL: 2.56-3.55 Species: Opercularia coarctata
- Duration:
- 24 h
- Dose descriptor:
- other: LC50
- Effect conc.:
- 3.78 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- element
- Basis for effect:
- other: mortality
- Remarks on result:
- other: 95%CL: 3.07-5.08 Species: Aspidisca lynceus
- Duration:
- 24 h
- Dose descriptor:
- other: LC50
- Effect conc.:
- 2.29 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- element
- Basis for effect:
- other: mortality
- Remarks on result:
- other: 95%CL: 2.01-2.61 Species: Vorticella convallaria
- Duration:
- 24 h
- Dose descriptor:
- other: LC50
- Effect conc.:
- < 1.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- element
- Basis for effect:
- other: mortality
- Remarks on result:
- other: Species: Trochilia minuta
- Duration:
- 24 h
- Dose descriptor:
- other: LC50
- Effect conc.:
- < 1.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- element
- Basis for effect:
- other: mortality
- Remarks on result:
- other: Species: Chilodonella uncinata
- Duration:
- 24 h
- Dose descriptor:
- other: LC50
- Effect conc.:
- < 1.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- element
- Basis for effect:
- other: mortality
- Remarks on result:
- other: Species: Euplotes sp.
- Duration:
- 24 h
- Dose descriptor:
- other: LC50
- Effect conc.:
- 2.81 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- element
- Basis for effect:
- other: mortality
- Remarks on result:
- other: 95%CL: 2.28-3.55 Species: Voricella octava
- Details on results:
- no additional remarks
- Reported statistics and error estimates:
- LC50s were determined using a probit analysis computer (Finney, 1978)
Any other information on results incl. tables
LC50 determined for each type of organisms
Type of test organisms |
LC50 (mg/L) |
Opercularia minima |
5 |
Opercularia coarctata |
3.28 |
Aspidisca lynceus |
3.78 |
Vorticella convallaria |
2.29 |
Trochilia minuta |
<1.5 |
Chilodonella uncinata |
<1.5 |
Euplotes sp. |
<1.5 |
Voricella octava |
2.81 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The test method is well described, and a LC50 was determined for this test species. Values are based on measured Pb-levels. Due to the followed test method, the results are more field data than lab data.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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