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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 204-927-3 | CAS number: 129-00-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study, meets national standard methods, limited documentation, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: AFNOR (draft, not further specified)
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Pyrene
- EC Number:
- 204-927-3
- EC Name:
- Pyrene
- Cas Number:
- 129-00-0
- Molecular formula:
- C16H10
- IUPAC Name:
- pyrene
- Details on test material:
- - Name of test material (as cited in study report): pyrene
- Molecular formula (if other than submission substance): C16H10
- Molecular weight (if other than submission substance): 202
- Substance type: organic
- Physical state: solid
- Source: no data
- Analytical purity: no data
- Typical concentration in coal-tar pitch: 0.94 - 1.48 % (data from NL 2008: Coal-Tar Pitch, Annex-XV dossier )
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES (data from NL 2008: Coal-Tar Pitch, Annex-XV dossier, and WHO 1998: EHC 202, if not otherwise indicated)
- Vapour pressure: 0.6 - 1.0*10^-3 Pa (at 25 °C)
- Water solubility: in freshwater, approx. 125 - 135 µg/L (20 - 25 °C);
soluble portion of the WSF of coal-tar pitch (loading 100 mg/L): 158 - 326 ng/L (mean 240 ng/L, n = 5)
[see 4.8: NOACK 2009_WSF, 100 mg/L nominal]
- Henry's law constant: 1.4 Pa*m3/mol (at 25 °C)
- log Pow: = 5.0 - 5.2
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- no data
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solution close to water saturation used for dilution series (no detailed data, see Report p. 34)
- Eluate: no
- Differential loading: no
- Controls: with and without vehicle
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no data
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): no data
Test organisms
- Test organisms (species):
- Ceriodaphnia dubia
- Details on test organisms:
- TEST ORGANISM
- Source: INERIS
- Age of parental stock (mean and range, SD): <24 h
- Feeding during test
- Food type: Chlorella vulgaris / Pseudokirchneriella subcapitata / fish diet
- Offspring: Males were counted and removed from the test medium.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
Test conditions
- Hardness:
- 240 mg/L (as CaCO3)
- Test temperature:
- 25 +- 2°C
- pH:
- 8.1 +-0.4
- Dissolved oxygen:
- >7.0 mg/L
- Nominal and measured concentrations:
- Nominal and analytical values not documented.
Analytical: calculation of the geometric mean from measured concentrations in fresh and spent medium,
based on an arithmetic model (OECD 1999) (see Report, p. 44/45). - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed (screw cap)
- Material, size, headspace, fill volume: glass vessel (90 mL), test volume 50 mL
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): 1x/d, 5x/wk (each workday)
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- No. of vessels per vehicle control (replicates): none
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: natural mineral water (Evian)
- Ca/Mg ratio: approx. 3:1
- Conductivity: 460 +-100 µs/cm
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark
- Light intensity: 500 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality adults and neonates, abortions, reproduction
VEHICLE CONTROL PERFORMED: yes
RANGE-FINDING STUDY
- Test concentrations: no data - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 7 d
- Dose descriptor:
- EC10
- Effect conc.:
- 2.1 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- dissolved
- Remarks:
- test material
- Basis for effect:
- other: mortality and reproduction
- Duration:
- 7 d
- Dose descriptor:
- EC10
- Effect conc.:
- 2.1 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Remarks:
- test material
- Basis for effect:
- other: mortality and reproduction
- Remarks on result:
- other: 95% C.I.: 1.3 - 3.1 µg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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