Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 272-271-5 | CAS number: 68784-75-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04 January 2010-06 January 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study has been performes according to OECD and/or EC guidelines and according to GLP principles.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, December 14, 2000.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all test concentrations and the control. Inaddition, the filter used for preparation of the test solutions was retained for possible analysis of the undissolved residue
- Sampling method:
Frequency at t=0 h and t=48 h
Volume 4.8 ml from the approximate centre of the test vessels
Storage Samples were stored in a freezer until analysis.
At the end of the exposure period, the replicates were pooled at each concentration before sampling - Vehicle:
- no
- Details on test solutions:
- Preparation started with a loading rate of 100 mg/l applying one day of magnetic stirring to reach maximum solubility of the test substance in the test medium. The obtained aqueous mixture was filtered through a 0.45 µm membrane filter (Whatman, rc 55) and the clear and colourless filtrate was used as the highest test concentration. The lower test concentrations were prepared by subsequent dilutions of the filtrate in test medium.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (Crustacea, Cladocera) (Straus, 1820)
- Source: In-house laboratory culture with a known history.
- Age at study initiation: < 24 hours
- Method of breeding: With newborn daphnids, i.e. less than 3 days old, by placing about 250 of them into 5 litres of medium in an all-glass culture vessel.
- Feeding during test: No - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 180 mg/l expressed as CaCO3
- Test temperature:
- between 20.6 and 21.7°C
- pH:
- Between 7.4 and 7.5
- Dissolved oxygen:
- Between 8.6 and 9.3 mg/l
- Salinity:
- not applicable
- Nominal and measured concentrations:
- The measured concentration of Barium in the samples taken from the undiluted filtrate at the start of the test was 0.57 mg/l. This concentration remained stable throughout the test period (99% of initial at the end of the test). The concentration of Lead was below the linear range of the calibration curve and therefore reported as lower than 0.01 µg/l. The analysis of the filter used for the preparation of the highest test concentration showed a relatively high content of lead.
- Details on test conditions:
- - Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass, 100 ml, normal headspace
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 for the highest concentration, 2 for the lower test concentrations
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Adjusted ISO medium: the following chemicals (analytical grade) are dissolved in tap water purified by Reverse Osmosis (RO-water, GEON Waterbehandeling, Berkel-Enschot, The Netherlands):
Macro salts: CaCl2.2H2O : 211.5 mg/l
MgSO4.7H2O: 88.8 mg/l
NaHCO3 : 46.7 mg/l
KCl : 4.2 mg/l
The hardness: 180 mg/l expressed as CaCO3 and the pH: 7.7 ± 0.3.
- Culture medium different from test medium: yes, for culturing M7 medium is used
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours photoperiod daily
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : EC50 t=24 and t=48 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Test concentrations:0, 0.1, 1.0, 10 and 100% of a 0.45 µm filtered solution prepared at a loading rate of 100 mg/l - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (K2Cr2O7)
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 other: mg/l (loading rate)
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 100% of the 0.45 µm filtered solution prepared at a loading rate of 100 mg/l
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 other: mg/l (loading rate)
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 100% of the 0.45 µm filtered solution prepared at a loading rate of 100 mg/l
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.57 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- element
- Remarks:
- dissolved barium in fitrate
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.57 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- element
- Remarks:
- dissolved barium in fitrate
- Basis for effect:
- mobility
- Details on results:
- Behavioural abnormalities: no
- Mortality of control: no
- Other adverse effects control: no
No acute immobilisation was observed at any concentration tested. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50/LC50:
The 24h-EC50 was 0.53 mg/l with a 95% confidence interval between 0.46 and 0.64 mg/l.
The 48h-EC50 was 0.38 mg/l with a 95% confidence interval between 0.31 and 0.46 mg/l. - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study Silicic acid(H2Si2O5), barium salt(1:1), lead-doped did not induce acute immobilisation of Daphnia magna exposed to a filtered solution prepared at a loading rate of 100 mg/l. The actual concentration of dissolved Barium in this filtrate was 0.57 mg/l.
The 48h-EC50 was beyond the range tested and therefore exceeded the solubility limit of Silicic acid(H2Si2O5), barium salt(1:1), lead-doped in test medium.
Reference
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.57 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)