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EC number: 620-582-5 | CAS number: 301341-58-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04.12.2012 to 07.12.2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Auf dem Aberg 1, D-57392 Schmallenberg, Germany
Test material
- Reference substance name:
- dipotassium 2-dodecanamidoacetate 2-tetradecanamidoacetate
- EC Number:
- 620-582-5
- Cas Number:
- 301341-58-2
- Molecular formula:
- R-CO-NH-CH2-COOK (Note: "R-CO-" is a fatty acid residue)
- IUPAC Name:
- dipotassium 2-dodecanamidoacetate 2-tetradecanamidoacetate
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Ajinomoto Co., Inc., 15-1 Kyobashi 1-Chome, Chuo-Ku, Tokyo 104-8315 Japan/Lot/Batch No.: 110825
- Expiration date of the lot/batch: 25.08.2014
- Purity test date: 24.01.2012
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: dark and cool place, protected from light
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: no
- Preliminary purification step (if any): no
- Final dilution of a dissolved solid, for the definitive test stock liquid or gel: The final test concentrations were obtained by dilution of the stock solution
- Final preparation of a solid: For the definitive test a stock solution was prepared by weighing 200 mg of the test item and dissolving in 2 L of dilution water.
FORM AS APPLIED IN THE TEST (if different from that of starting material): aqueous solution
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: stock solution was prepared in dilution water by weighing 200 mg of the test item, transferring it into a glass bottle and dissolving it in 2 L of dilution water. The solution was stirred for 2 h at about 300 rpm. The final test concentrations were obtained by dilution.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (water flea)
- Strain: STRAUS
- Source: German Federal Environment Agency, Institut für Wasser-, Boden- und Lufthygiene. Specimens used in the test were bred in the laboratory at Fraunhofer IME
- Age of parental stock (mean and range, SD): <24 h
- Method of breeding: Adult Daphnia, at least 3 weeks old, were separated from the stock population by sieving. Batches of 30 - 50 animals were held at room temperature in ca. 1.8 L of dilution water for one week. Algae growing in the log-phase were centrifuged and the pellet was re-suspended in a few mL of medium. Thirty millilitres of this suspension was given to 1 L of medium. The water was changed once per week.
- Feeding during test: no
- Food type: algal suspension (Desmodesmus subspicatus) and LiquizellR (HOBBY)
- Frequency: daily
ACCLIMATION
- Acclimation period: not necessary
- Acclimation conditions (same as test or not): same conditions
- Health during acclimation (any mortality observed): yes
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20.3°C
- pH:
- 7.85 – 8.40
- Dissolved oxygen:
- 5.1 – 9.2 mg/L
- Nominal and measured concentrations:
- 100.0, 50.0, 25.0, 12.5, 6.25 mg/L (nominal concentrations)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beaker
- Material, size, headspace, fill volume: glass/60 mL/-/50 mL
- Aeration: not during the test
- Type of flow-through (e.g. peristaltic or proportional diluter): test conducted under static conditions
- Renewal rate of test solution (frequency/flow rate): the test solution was not changed during the test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Purified drinking water (filtration with activated charcoal, passage through a lime-stone column and aeration) was used according to guideline
- Alkalinity: 1.8 mmol/L
- Ca/Mg ratio: 4:1
- Conductivity: 206.4 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: regularly (not further specified)
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8 h light/dark cycle
- Light intensity: 499 – 509 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Immobilisation and sublethal effects, determined after 24 and 48 h
VEHICLE CONTROL PERFORMED: no (no vehicle used)
RANGE-FINDING STUDY: performed
- Test concentrations: 1, 10, 100 mg/L nominal
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- 24h-EC50 = 1.03 mg/L
- Reported statistics and error estimates:
- The NOEC and LOEC values were determined using statistical methods like ANOVA followed by Williams' Multiple Sequential t-Test. The computer program ToxRat(R)Professional was used for the statistical evaluations.
Any other information on results incl. tables
No statistically significant effects on immobilisation of Daphnia magna were observed up to the highest test concentration.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of acylglycinate GCK-11 -T to Daphnia magna was determined in a study conducted according to guideline. In this static test the 48 h NOEC-, LOEC-, and EC50 -values were determined to be >=100 , >100, and >100 mg/L nominal, respectively.
- Executive summary:
The acute toxicity of acylglycinate GCK-11 -T to Daphnia magna was investigated in a study conducted according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) and EU Method C.2 (Acute Toxicity for Daphnia). In this static test, 20 daphnids per concentration were exposed to nominal concentrations of 0 (control), 6.25, 12.5, 25.0, 50.0, and 100.0 mg/L for 48 h. The 48 h NOEC-, LOEC-, and EC50 -values were determined to be >=100, >100, and >100 mg/L nominal, respectively.
This study is classified as reliable without restriction and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.
Results Synopsis
Test organism: Daphnia magna (<24 h old)
Test type: static
48 h EC50>100 mg/L nominal
Endpoint(s) effected: immobilisation
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