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Diss Factsheets
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EC number: 932-164-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 10th February 2003 to 16th May 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Water samples were taken at 0, 24, 48, 72 and 96 h for quantitative analysis.
- Details on test solutions:
- Samples are extracted with dichloromethane, dryed with sodium sulphate and the sovent was evaporated. Then the analysis was done in as solution in acetone.
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- Fish were obtained from Brow Well Fisheries, Hebden, UK. Fish were maintained in a glass fibre tank.
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Sub-lethal effects were observed at concentrations of 3.2 mg/L and above. These responses were fish swimming at the bottom of the test vessel, swimming at the surface, loss of equilibrium and presence of moribund fish.
- Hardness:
- 133 mg/L as CaCO3
- Test temperature:
- 14 °C
- pH:
- 7.6 - 7.7
- Dissolved oxygen:
- 81-91 air saturation value
- Salinity:
- 441 µS/m
- Nominal and measured concentrations:
- Nominal Measured
1 0.466
1.8 0.766
3.2 1.64
5.6 5.2
10 8.68 - Details on test conditions:
- Concentrations for the definitive tests are chosen according to the results for the range-finding test. LC50-values are calculated from the results using various statistical methods.
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.77 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: The NOEC is based upon zero mortalities and the absence of any sub-lethal effects of exposure at this concentration
- Duration:
- 96 h
- Dose descriptor:
- other: 95% CL
- Effect conc.:
- >= 1.6 - <= 5.2 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL. Concentrations resulting in 0% and 100% mortalities respectively
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.9 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Given the decline in measured test concentrations it was considered justifiable to base the results on the time-weighted mean measured concentrations of the test media to give a "worst case" analysis of the data.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of the test material to the freshwater fish rainbow trout (Oncorhynchus mykiss) has been investigated. Based on the time-weighted mean measured test concentrations the acute toxicity of the test material to rainbow trout gave a 96-Hour LC50 value of 2.9 mg/L with 95% confidence limits of 1.6-5.2 mg/L. The No Observed Effect Concentration was 0.77 mg/L.
- Executive summary:
A study was performed to assess the acute toxicity of the test material to rainbow trout (Oncorhynchus mykiss). The method followed that described in the OECD guidelines for Testing of Chemicals (1992) No. 203, “Fish, Acute Toxicity Test” referenced as Method C.1 of Commission Directive 92/69/EEC.
Following a preliminary range-finding test, fish were exposed, in groups of twenty, to an aqueous dispersion of the test material over a range of concentrations of 1.0, 1.8, 3.2, 5.6 and 10mg/L for a period of 96 hours at a temperature of 13.9 to 14.5 °C under semi-static test conditions. The test was conducted in the dark. The number of mortalities and any sub-lethal effects of exposure in each test vessel and control vessel were determined 3 and 6 hours after the start of exposure and then daily throughout the test until termination after 96 hours.
Given the decline in measured test concentrations it was considered justifiable to base the results on the time-weighted mean measured test concentrations of the test media to give a “worst case” analysis of the data. The 96-Hour LC50 based on the time weighted mean measured test concentrations was 2.9 mg/L with 95% confidence limits of 1.6-5.2 mg/L. The No Observed Effect Concentration was 0.77 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- From 6 September 1991 to 25 October 1991
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Concentrations for the definitive tests are chosen according to the results for the range-finding test. LC50-values are calculated from the results using various statistical methods.
- Test organisms (species):
- Leuciscus idus melanotus
- Details on test organisms:
- TEST ORGANISM
- Strain: Leuciscus idus melanotus
- Source: Fischzucht Eggers, W-2354 Hohenwestedt
- Length at study initiation (length definition, mean, range and SD): 5-8 cm - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 20
- pH:
- 8.38
- Dissolved oxygen:
- 7.88
- Details on test conditions:
- Preparation of stock solution with a concentration of 1g/L of test substance by dilution with drinking water.
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 10 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 13 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 16 mg/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- LC50(96h)= 13 mg/L
- Executive summary:
According to OECD Guideline 203 (Fish, Acute toxicity Test)
The acute toxicity of test material to the Leuciscus idus melanotus was determinate in a 96 -hour, static, aquatic effects test. A total of 10 fishes were exposed to each of six test substance concentrations. Concentrations were 2, 4, 10, 14 and 16 mg/L. The LC50(96h) was 13 mg/L.
Referenceopen allclose all
Description of key information
According to OECD Guideline 203 (Fish, Acute toxicity Test).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 2.9 mg/L
Additional information
There are two studies available for this endpoint. The key study was chosen as the study was performed under GLP-conditions (reliability 1). This study was additionally resulting in a higher toxicity of the substance and represents the worst case for acute toxicity to fish. The key study gave an LC50(96h) of 4.2 mg/L (based on nominal test concentrations). The LC50 (96h) based on time-weighted mean measured test concentrations was 2.9 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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