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EC number: 233-195-8 | CAS number: 10061-01-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18th October 1988 - 2nd March 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study conducted in accordance with generally accepted scientific principles, possibly with incomplete reporting or methodological deficiencies, which do not affect the quality of the relevant results.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- yes
- Remarks:
- As the test substance is volatile, it was injected into the oxygen uptake measuring apparatus during sludge addition. The contact time between the test substance and activated sludge was therefore reduced from three hours to 10 minutes
- Principles of method if other than guideline:
- Part C: Methods for the Determination of Ecotoxicity, Official Journal of the European Communities No. L 133 31, 118-122 (1988).
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- (Z)-1,3-dichloropropene
- EC Number:
- 233-195-8
- EC Name:
- (Z)-1,3-dichloropropene
- Cas Number:
- 10061-01-5
- Molecular formula:
- C3H4Cl2
- IUPAC Name:
- 1,3-dichloroprop-1-ene
- Details on test material:
- NAME: CIS-l,3-DICHLOROPROPENE
BATCH (& OTHER) NUMBERS SNC NO: 1986; 88009
TOXICOLOGY REF. NUMBER: ST88/253
SOURCE: Shell Nederland Raffinaderij B.V., Rotterdam
DATE RECEIVED: 18th October 1988
APPEARANCE: Clear colourless liquid
CHARACTERIZATION: Actual Analysis
Cis-l,3-DCP (SMS 2060) 94.51% and 95.45%
(LPM 3998) 96.9 %
(LPM 4922) 97.51%
Trans-1,3-Dichloropropene (LPM 3998) 1. 5 %
Cis+Trans (LPM 3998) 98.4%
3,3-DCP (LPM 3988) 0%
Benzene 0%
1,2-Dichloropropane 0.25%
2,3-Dichloropropane 0.01%
Water % m/m (LPM 4687) 0.03%
Edenol D81 (LPM 4389) 1.0%
Origin of sample CAS-CMDU-S
DENSITY: 1.224 g/ml at 15.2 ºC
(Measured at SRC using Paar DMA 35 density meter)
DATE RELEASED: 18th November 1988 and 30th May 1989
Storage of this test substance
Following its arrival in Compound Control this test substance was stored in the dark at ambient temperature under nitrogen.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- NDA
Test solutions
- Vehicle:
- no
- Details on test solutions:
- 32 mg/l, 100 mg/l, 320 mg/l and 1 000 mg/l solutions
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Samples of activated sludge from Canterbury Sewage Works were aerated for three hours at 20 °C in the presence of a standard amount of synthetic sewage feed.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- N/A
Test conditions
- Hardness:
- NDA
- Test temperature:
- 20 ºC
- pH:
- NDA
- Dissolved oxygen:
- NDA
- Salinity:
- NDA
- Nominal and measured concentrations:
- Nominal: 32, 100, 320 and 1000 mg/l
- Details on test conditions:
- Flasks amended with a range of concentrations of a reference inhibitor (3,5-dichlorophenol), and flasks containing sludge plus feed but no inhibitory substances ("controls") were run in parallel. After a contact time of three hours, the oxygen consumption (respiration) rate of each reference inhibitor and control sample was measured. As cis-1,3-dichloropropene is volatile, aeration for three hours would have resulted in substantial losses of the test substance. The compound was therefore injected into the measuring vessel during the addition of sludge plus feed (previously incubated for three hours). The contact time between the activated sludge and the test substance was therefore the duration of the respiration rate measurement (7-10 minutes) and not three hours.
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 10 min
- Dose descriptor:
- EC50
- Effect conc.:
- 279 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The effect of cis-l,3-dichloropropene on the respiration rate of activated sludge is given in Table 1.
- Results with reference substance (positive control):
- As the EC50 for the reference inhibitor 3,5-dichlorophenol was within the range 5-30 mg/l (8.5 mg/l), and the relative standard deviation of the two controls was <15%, the test was considered to be valid.
- Reported statistics and error estimates:
- The respiration rate of the activated sludge is calculated from the linear portion of the oxygen consumption curve and is given in units of mg O2/g sludge/h. Percentage inhibition of respiration rate is calculated at each concentration of test or reference substance using the equation:
% Inhibition = (1 - 2Rs / (Rc1 + Rc2)) x 100
where Rs = respiration rate at tested concentration of test or reference substance,
RCI - respiration rate of control "C1",
RC2 - respiration rate of control "C2".
If a suitable range of inhibition was observed over the range of test or reference substance concentrations, then inhibition curves of % inhibition at each concentration against log10 concentration will be plotted. The concentration of substance which inhibits respiration by 50 % (EC50) can then be interpolated from this curve.
Any other information on results incl. tables
Table 1: Effect of cis-l,3-dichloropropene on the respiration rate of activated sludge
Substance |
Concentration (mg/L) |
% Inhibition |
Cis-1,3-Dichloropropene
EC50 |
32 |
37 |
100 |
37 |
|
320 |
56 |
|
1000 |
58 |
|
279 |
|
|
3,S-dichlorophenol
EC50 |
1 |
-12 |
3.2 |
18 |
|
10 |
58 |
|
32 |
83 |
|
100 |
91 |
|
8.5 |
|
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The effect of cis-1,3-dichloropropene on the respiration rate of activated sludge has been assessed by a modified version of the EEC procedure. As the test substance is volatile, it was injected into the oxygen uptake measuring apparatus during sludge addition. The contact time between the test substance and activated sludge was therefore reduced from three hours to the duration of the respiration.rate measurement (approximately 10 minutes).
The EC50 for cis-l,3-dichloropropene under these conditions was found to be 279 mg/l. - Executive summary:
The aim of this study was to determine the effect of cis-l ,3-dichloropropene on the respiration rate of activated sludge. As the test substance is volatile, it was injected into the oxygen uptake measuring apparatus during sludge addition. The contact time between the test substance and activated sludge was therefore reduced from three hours to the duration of the respiration rate measurement (approximately 10 minutes).
The EC50 for cis-l ,3-dichloropropene under these conditions was found to be 279 mg/l.
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