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Diss Factsheets
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EC number: 200-580-7 | CAS number: 64-19-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP, non guideline study, published in peer reviewed literature, limitations in reporting but otherwise adequate for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Comparison of the Toxicity Thresholds of Water Pollutants to Bacteria, Algae, and Protozoa in Cell Multiplication Inhibition Test
- Author:
- Bringmann, G. and Kuhn, R.
- Year:
- 1 980
- Bibliographic source:
- Water Research 14(3) 231-241
Materials and methods
- Principles of method if other than guideline:
- The effect of the test substance on the inhibition of the multiplication of bacterial cells of the genus Pseudomonas was investigated. Cultures of the test organisms were exposed to a series of test concentrations of the test substance for 16 hours and the effects on cell multiplication measured using a turbidimetric technique. Results are reported relative to the blank controls.
- GLP compliance:
- no
- Remarks:
- predates GLP
Test material
- Reference substance name:
- Acetic acid
- EC Number:
- 200-580-7
- EC Name:
- Acetic acid
- Cas Number:
- 64-19-7
- Molecular formula:
- C2H4O2
- IUPAC Name:
- acetic acid
- Details on test material:
- No data reported
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No data reported
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- The concentration of the bacterial suspension was measured turbidimetrically at 0 and 16 hours
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Test solutions prepared from a stock solution, with each dilution containing 1 part v/v test substance solution in 2^0 to 2^14 v/v double distilled water. Nutrient medium, trace element solution and vitamin solution were added to each test vessel together with 10ml of bacterial suspension.
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- Stock cultures of the test strain kept on the nutrient for stock and preliminary cultures in agar slant tubes. New stock cultures prepared at 1 week intervals. The inoculated stock culture incubated at 25°C for 24h
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 16 h
- Post exposure observation period:
- None
Test conditions
- Hardness:
- No data reported
- Test temperature:
- 25°C
- pH:
- authors state that the solution of test substance was neutralised by the addition of a minimal volume of acid or alkaline solution
- Dissolved oxygen:
- No data reported
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- No data reported
- Details on test conditions:
- Four parallel dilution series in 300ml Erlenmeyer flasks, stoppered with cotton-lined plastic caps. Both inoculated and non-inoculated dilution series left at 25°C for 16h
- Reference substance (positive control):
- no
- Remarks:
- No data reported
Results and discussion
Effect concentrations
- Duration:
- 16 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 150 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
- After 16 hours the extinction of the monochromatic radiation at 436nm in a 10mm layer in the inoculated dilution series was measured. The lowest concentration having a mean extinction value ≥3 below the mean for non-toxic dilutions is reported as the EC3.
- Results with reference substance (positive control):
- No data reported
- Reported statistics and error estimates:
- No data reported
Applicant's summary and conclusion
- Validity criteria fulfilled:
- no
- Remarks:
- non-standard test
- Conclusions:
- An 16h EC3 of 850mg/l has been reported for Pseudomonas putida . This can be considered to be the NOEC.
- Executive summary:
An 16h EC3 of 850mg/l has been reported for Pseudomonas putida . This can be considered to be the NOEC. Although this study is non-GLP and is non guideline, it is considered suitable for use as a key study. The study investigates the effects of the test substance on a standard test organism. A series of exposure concentrations were used and results reported as the inhibition of culture growth relative to controls.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.