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EC number: 613-953-8 | CAS number: 66603-10-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Study initiation date: 14.10.2004; Experimental start date: 18.10.2004; Experimental termination date: 16.11.2004; Study completion date: 25.01.2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 217 (Soil Microorganisms: Carbon Transformation Test)
- Version / remarks:
- adapted 21 st January 2000
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Guidance Document on Terrestrial Ecotoxicology Under Council Directive 91/414/EEC. SANCO/10329/2002
- Version / remarks:
- rev. 2 final. Working Document (Draft), 17 October 2002.
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Cyclohexylhydroxydiazene 1-oxide, potassium salt
- EC Number:
- 613-953-8
- Cas Number:
- 66603-10-9
- Molecular formula:
- C6H11KN2O2
- IUPAC Name:
- Cyclohexylhydroxydiazene 1-oxide, potassium salt
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- Lot/Batch number: VM 2012
Purity: 30 % (30.41 % analysed) in water
Formulation type: aqueous solution
Appearance: yellowish liquid
Density: 1.12 g/cm³ at 25 °C
Received: 27.09.2004
Expiration date: 28.07.2006
Chemical name: N-Cyclohexyldiazeniumdioxy-potassium
Solubility in water: Soluble
Storage conditions: room temperature (> 15 °C)
Stability: expected to be stable at least for 2 years if stored under the cited storage conditions
Test concentrations: 20 mg/kg, 100 mg/kg soil dry weight (d.w.)
Sampling and analysis
- Analytical monitoring:
- not specified
- Details on sampling:
- Soil samples (100 g d.m.) are taken at intervals of 3 hours, 7, 14 and 28 days after application of the test item. Before the beginning of the test, the optimal glucose concentration was determined
Test substrate
- Vehicle:
- yes
- Remarks:
- water
- Details on preparation and application of test substrate:
- AMENDMENT OF SOIL
- Type of organic substrate: Biologically active agricultural soil: loamy sand soil.
SOIL REMOVAL, SOIL PREPARATION AND SOIL STORAGE
Corresponding to the OECD Guideline an agriculturally utilized soil was selected. The soil was removed to a depth of 20 cm as mixed samples. Because the soil was wet it was carefully dried at room temperature. Afterwards, the soil was passed through a 2 mm mesh sieve. Subsequently, the soil was stored at a temperature of 4 °C in containers under aerobic conditions in the dark.
TEST PREPARATION
The examination was performed with one soil.
The test item was applied at a test concentration of 20 mg/kg soil d.w. and a fivefold test concentration of 100 mg/kg soil d.w. The short-term respiration was determined on day 0 (after approximately 3 hours) and at intervals of 7, 14, and 28 days after application.
Test organisms
- Test organisms (inoculum):
- soil
Study design
- Total exposure duration:
- 28 d
Test conditions
- Test temperature:
- (20 ± 2) °C in a climatic room
- Moisture:
- Water content of soil: approximately 45 % of WHC
- Details on test conditions:
- TEST SYSTEM
- Testing facility: Biochem Agrar
- Test container (type, material, size): Soil samples were incubated at 20 °C ± 2 °C while stored in new plastic vessels.
- Amount of soil: 1200 g soil d.w. per vessel
- No. of replicates per concentration: 3
- No. of replicates per control: 3
SOIL INCUBATION
- The incubation of the prepared soil was carried out in new plastic vessels ( 4 L)
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographical reference of sampling site (latitude, longitude): 12.694435960 degrees East, 51.403774567 degrees North
- History of site: fallow ground
- Vegetation cover: fallow ground
- Treatments with pesticides or fertilizers: none
- Accidental contamination: none
- Depth of sampling: The soil was removed to a depth of 20 cm as mixed samples.
- Humus content [%]: 2.51
- Nmin [mg/100 g soil d.w.]: 0.99
- % sand: 50.2
- % silt: 39.1
- % clay: 10.7
- Soil taxonomic classification: loamy sand soil.
- pH (in water): 6.6 before application, 6.4 after 28 days
- Initial nitrate concentration for nitrogen transformation test (mg nitrate/kg dry weight):
- Maximum water holding capacity (in % dry weigth): 45 % of WHC
- Cation exchange capacity (mmol/kg): 12.15
- Pretreatment of soil: Because the soil was wet it was carefully dried at room temperature. Afterwards, the soil was passed through a 2 mm mesh sieve.
Subsequently, the soil was stored at a temperature of 4 °C in containers under aerobic conditions in the dark.
- Storage (condition, duration): the soil was stored at a temperature of 4 °C in containers under aerobic conditions in the dark.
- Initial microbial biomass as % of total organic C: 24.37 = 1.67 % compared to Corg.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : pH-value, water content, O2 consumption. Soil samples (100 g soil d.w.) were taken at intervals of 3 hours, 7, 14 and 28 days after application of the test item.
RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- yes
- Remarks:
- dinoseb acetate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 28 d
- Dose descriptor:
- other: O2 consumption- deviation from control
- Effect conc.:
- 1.9 other: - % deviation from control with a test concentration of 100 mg/kg soil dry weight
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: O2 consumption
- Remarks on result:
- other: No adverse effects on carbon transformation
- Key result
- Duration:
- 28 d
- Dose descriptor:
- other: O2 consumption deviation - from control
- Effect conc.:
- 0.3 other: 0.3 % deviation from control with a test concentration of 20 mg/kg soil dry weight
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: O2 consumption - deviation from control
- Remarks on result:
- other: No adverse effects on carbon transformation
- Details on results:
- See table below
- Results with reference substance (positive control):
- The reference item dinoseb acetate produced in the soil the expected level of effect (25.4 % inhibition after 28 days).
- Reported statistics and error estimates:
- Calculation of mean values per treatment, standard deviations and coefficients of variation.
Any other information on results incl. tables
Effects on carbon transformation in soil after treatment with Xyligen LP 15684 |
||||||||
Days after application |
Control |
Xyligen LP 15684 |
Xyligen LP 15684 |
|||||
O2consumption (mg/kg soil d.w./h) |
CV (%) |
O2consumption (mg/kg soil d.w./h) |
CV |
Deviation from control (%) |
O2consumption (mg/kg soil d.w./h) |
CV |
Deviation from control (%) |
|
0 |
7.99 |
0.87 |
8.15 |
0.41 |
+ 2.0 |
7.76 |
0.96 |
-2.9 |
7 |
8.06 |
0.67 |
8.06 |
0.35 |
0.0 |
7.83 |
0.65 |
-3.0 |
14 |
7.78 |
0.99 |
7.83 |
1.45 |
+ 0.6 |
7.61 |
0.49 |
-2.1 |
28 |
7.31 |
1.75 |
7.28 |
0.51 |
- 0.3 |
7.17 |
2.22 |
-1.9 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Validity criteria:
The coefficients of variation in control were maximum 1.75 % and thus fulfilled the demanded range (≤ 15 %). In the most recent test, dated 14.01.04 – 11.02.04, the toxic standard Dinoseb acetate caused a reduction of the O2-consumption of 25.4 % after 28 days and thus demonstrated the sensitivity of the test system.
No short-term and long-term effects (OECD 217) on the carbon transformation in the field soil tested up to a concentration of 100 mg Xyligen LP 15684 per kg soil dry weight.
No short-term and long-term effects (OECD 217) on the carbon transformation in the field soil tested up to a concentration of 100 mg Xyligen LP 15684 (30 % K-HDO in water) per kg soil dry weight. - Executive summary:
Based on the results of this study Xyligen LP 15684 (K-HDO 30 % in water) caused no short-term and long-term effects (OECD 217) on the carbon transformation in the field soil tested up to a concentration of 100 mg Xyligen LP 15684 per kg soil dry weight.
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