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EC number: 613-953-8 | CAS number: 66603-10-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22.03.1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 1993
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Cyclohexylhydroxydiazene 1-oxide, potassium salt
- EC Number:
- 613-953-8
- Cas Number:
- 66603-10-9
- Molecular formula:
- C6H11KN2O2
- IUPAC Name:
- Cyclohexylhydroxydiazene 1-oxide, potassium salt
- Test material form:
- liquid
- Details on test material:
- Xyligen 30F is a 30% aqueous solution of K-HDO.
Constituent 1
- Specific details on test material used for the study:
- Test substance K-HDO, techn. 30%ig
Chemical name N-Cyclohexyldiazeniumdioxy-potassium
Molecular formula C6H13N203K
Molecular weight 200.28 (pure substance)
Batch number 74 N 9702
Odour Weak odour
Colour Colourless
Aggregate state Liquid
Density 1.13
Solubility in water Fully miscible
Instability against Acids
Storage Room temperature, darkness
Homogeneity Homogenous
Purity of the test substance Technical purity, 30 % active substance content
Sampling and analysis
- Analytical monitoring:
- not required
- Remarks:
- The test substance is fully miscible with water
- Details on sampling:
- O2 consumption has been determined after 30 minutes.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- test concentrations: 2.8, 7.8, 39, 84, 202, 403, 812 mg/L
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Inoculum: Activated sludge from laboratory sewage treatment plants running with municipal and synthetic waste water
Concentration in the test: 1 g/L dry substance
Synthetic nutrition medium: 8 ml/ test assay, 100-times concentrated OECD medium
Volume of the test assay: 250 ml
Incubation temperature: 20 ± 2°C
Study design
- Test type:
- not specified
- Water media type:
- other:
- Remarks:
- not applicable for an activated sludge test
- Limit test:
- no
- Total exposure duration:
- 30 min
- Post exposure observation period:
- Not required
Test conditions
- Hardness:
- not required
- Test temperature:
- not specified
- pH:
- not specified
- Dissolved oxygen:
- not specified
- Salinity:
- not required
- Conductivity:
- not required
- Nominal and measured concentrations:
- Results are given as nominal values.
- Details on test conditions:
- The test has been performed according to the annex of the EEC directive 88/302/EEC 18 Nov. 1987 (Activated sludge respiration inhibition test)
This method corresponds to:
– OECD guidelines for testing of chemicals Activated sludge, respiration inhibition test 209; Paris 1993
– International standards ISO 8192-(1994) Water quality – test for inhibition of oxygen consumption by activated sludge
In the short-term respiration test, the effect of a test substance on the respiration of aerobe microorganisms after a short exposure time (30 up to 80 minutes) will be tested. The effective concentrations at which the respiration compared to a control value without test substance is inhibited for 20, 50 and 80 % (EC20, EC50, EC80) are the determined test results.
The EC20 value is the limit concentration at which toxic effects in biological sewage treatment plants have to be expected. The test concentration used for biological degradation tests should not exceed the EC20 value. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- ca. 4.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 30 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 30 min
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 812 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- See tables below
- Results with reference substance (positive control):
- See tables below
- Reported statistics and error estimates:
- None reported
Any other information on results incl. tables
Results with test substance:
|
Reference values |
Mean value (reference substance) |
Test substance concentrations |
||||||||
Concentration of test substance (mg/L) |
- |
- |
- |
- |
812 |
403 |
202 |
84 |
39 |
7.8 |
2.8 |
O2consumption after 30 min. (mg O2/L·h) |
22 |
23 |
23 |
23 |
5 |
6 |
7 |
9 |
11 |
14 |
23 |
Inhibition of respiration (%) after 30 min. |
- |
- |
- |
- |
78 |
74 |
70 |
61 |
52 |
39 |
0 |
Results with positive control substance:
|
Reference substance (MV) |
Control substance |
||
Substance concentration (mg/L) |
|
1 |
10 |
100 |
O2consumption (mg O2/L x h) |
23 |
20 |
8 |
2 |
Respiration inhibition (%) |
0 |
-13 |
-65 |
-91 |
Effective concentrations EC-values:
|
EC20(mg/L) |
EC50(mg/L) |
EC80(mg/L) |
Maximum test concentration |
K-HDO, techn. 30% |
4.8 |
30 |
- |
812 |
3,5-Dichlorophenol |
ca. 1 |
ca. 5 |
ca. 38 |
1000 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- No respiration inhibition up to 4.8 mg/L
- Executive summary:
The effect of the test substance (30% K-HDO in water) on the respiration of aerobe microorganisms in activated sludge after a short exposure time of 30 has been tested. According to the results of the study the effective concentrations after 30 minutes are as follows:
EC20: 4.8 mg/L corresponds to 1.44 mg K-HDO/L (nominal)
EC50: 30 mg/L corresponds to 9 mg K-HDO/L (nominal)
EC80: > 812 mg/L* corresponds to > 243.6 mg K-HDO/L (nominal)
*At the highest concentration tested (812 mg/L 30% K-HDO in water) an inhibition of 78% was observed.
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