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EC number: 613-953-8 | CAS number: 66603-10-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Cyclohexylhydroxydiazene 1-oxide, potassium salt
- EC Number:
- 613-953-8
- Cas Number:
- 66603-10-9
- Molecular formula:
- C6H11KN2O2
- IUPAC Name:
- Cyclohexylhydroxydiazene 1-oxide, potassium salt
- Test material form:
- liquid
- Details on test material:
- 30% w/w aqueous solution of Cyclohexylhydroxydiazene 1-oxide, potassium salt
Constituent 1
- Specific details on test material used for the study:
- Name of the test substance: K-HDO, techn. 30%
Chemical Name: N-Cyclohexyldiazeniumdioxy-potassium
Molecular formula: C6H13N2O3K
Molecular weight (g/mol): 200.28 (pure substance)
Batch No.: 74 N 9702
Manufacturing date: 07.02.95
Odour: product specific
Colour: Colourless
State of aggregation: liquid
Density (g/cm³ at 20 °C): 1.13
Solubility in water (mg/L): miscible in each ratio
Instability: Acids
Remarks for storage: store at room temperature and in darkness
Homogeneity: yes, aqueous solution
Purity of test substance: technical grade, 30 % active ingredient content
pH: 10.5 (of the 30% aqueous solution)
Study design
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: Activated sludge from laboratory plants running with municipal and synthetic waste water
- Concentration of sludge: 1g/L dry matter content - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 2 mg/L
- Based on:
- act. ingr.
- Details on study design:
- Test Procedure
The static Zahn-Wellens test is a static method to determine the ultimate, potential aerobic biodegradation of a test substance (inherent biodegradability) and the elimination from water, respectively. The test substance, a defined inorganic medium and as inoculum activated sludge from a municipal sewage plant or laboratory plant were mixed and aerated at room temperature up to 28 days. Samples are taken in regular intervals and were analysed in this case with substance specific analysis. In addition to the samples with test substance, an assay to determine the blank value (no test substance) and a test with a reference substance was performed.
Reference substance
- Reference substance:
- diethylene glycol
Results and discussion
% Degradationopen allclose all
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- >= 98
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 57
- Sampling time:
- 3 h
- Details on results:
- Decrease of the test substance (substance specific analysis) after 28 days: 98 %
Decrease of the test substance (substance specific analysis) after 3 hours: 57 %
The test substance is well eliminated from water
See also tables below
BOD5 / COD results
- Results with reference substance:
- see tables below
Any other information on results incl. tables
Modified static Zahn-Wellens Test
Overview of the measured values
Test substance: K-HDO, techn. 30%; reference substance: diethylene glycol
Analysis: BW1, KS1: DOC after centrifugation
PS1: substance specific analysis after centrifugation
Measured values: BW1, KS1: [mg/L DOC], PS1: [mg/L K-HDO] and pH values of the test measurements
Date |
Day |
BW1 a b pH |
KS1 a b pH |
PS1 pH |
|||||
26.6.95 |
0 |
0.0 |
0.0 |
7.0 |
226 |
223 |
6.9 |
2 |
7.1 |
26.6.95 |
0.125 |
3.6 |
3.5 |
7.8 |
208 |
206 |
7.9 |
0.8 |
7.8 |
27.6.95 |
1 |
4.4 |
4.1 |
7.2 |
204 |
204 |
7.2 |
0.9 |
7.3 |
30.6.95 |
4 |
7.8 |
7.5 |
7.0 |
190 |
188 |
5.3 |
0.6 |
7.2 |
3.7.95 |
7 |
8.6 |
9.3 |
6.7 |
15.3 |
15.5 |
8.1 |
0.4 |
6.8 |
5.7.95 |
9 |
6.6 |
6.6 |
6.9 |
9.6 |
9.6 |
7.2 |
nv |
nv |
6.7.95 |
10 |
6.4 |
7.8 |
7.1 |
7.6 |
8.4 |
7.1 |
0.4 |
7.2 |
10.7.95 |
14 |
7.7 |
7.9 |
7.0 |
8.0 |
7.9 |
7.1 |
0.4 |
7.0 |
13.7.95 |
17 |
|
|
|
|
|
|
0.2 |
6.8 |
17.7.95 |
21 |
|
|
|
|
|
|
0.04 |
6.8 |
20.7.95 |
24 |
|
|
|
|
|
|
0.02 |
6.9 |
23.7.95 |
27 |
|
|
|
|
|
|
0.02 |
7.0 |
24.7.95 |
28 |
|
|
|
|
|
|
0.04 |
7.1 |
remarks: a/b = DOC single measurements
nv = this value is not determined because no sampling point occurred
DOC-measurements below the detection limit of 0.1 mg/L are presented in the table as 0 mg/L
The substance specific analysis of sample 95/0179/10/2BW1/6/00/00 yielded about 0.03 mg/l K-HDO. The tabulated PS1-values consider this result already.
The KS1 value (reference substance) was accidentally tested again at day 14 although this test was just finished at day 10.The result validates the previous values.
Degree of elimination D”(T) [%] KS1 (DOC-Analysis), PS1 (substance specific analysis)
Date |
Day |
KS1 202 mg/L DOC* |
PS1 2 mg/L K-HDO (active substance) |
26.6.95 |
0 |
0 |
0 |
26.6.95 |
0.125 |
-1 |
57 |
27.6.95 |
1 |
1 |
51 |
30.6.95 |
4 |
10 |
68 |
3.7.95 |
7 |
97 |
79 |
5.7.95 |
9 |
99 |
- |
6.7.95 |
10 |
100 |
79 |
10.7.95 |
14 |
100 |
79 |
13.7.95 |
17 |
|
90 |
17.7.95 |
21 |
|
98 |
20.7.95 |
24 |
|
99 |
23.7.95 |
27 |
|
99 |
24.7.95 |
28 |
|
98 |
Remarks: * = measured adjusted start concentration CA”
The degree of elimination D”(T) relates to the measured, adjusted start concentration CA”.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The elimination of K-HDO from water by absorption has been verified with this test; it is assumed that this will normally also take place in sewage plants and the environment.
- Executive summary:
The elimination of K-HDO from water by absorption has been verified with this test; it is assumed that this will normally also take place in sewage plants and the environment.
Criteria for validity:
Reference substance: diethylene glycol
Degree of degradation of the reference substance (% DOC): 97 after 7 days
Degradation of the reference substance after 14 days > 80%: yes
Test is valid: yes
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