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Diss Factsheets
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EC number: 202-974-4 | CAS number: 101-77-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
- Deviations:
- yes
- Remarks:
- In order to avoid false positive results by reaction of the test substance with nitrite, ammonia reduction was used too as endpoint for the determination of inhibiting effects beside the formation of nitrate.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on preparation and application of test substrate:
- AMENDMENT OF SOIL
- Type of organic substrate: Loamy sand according to DIN (supplied bei LuFa Speyer)
APPLICATION OF TEST SUBSTANCE TO SOIL
- Method: The test substance was grounded in a mortar. Than the required amount of the test substance for each test concentration were balanced with 5 g quartz sand as carrier (equal to 10 g/kg dry weight) and mixed well. The test substance was grounded in a mortar. Than the required amount of the test substance for each test concentration were balanced with 5 g quartz sand as carrier (equal to 10 g/kg dry weight) and mixed well. 14 portions of the prepared soil were blended with a mixer with quartz sand containing the test substance in the scheduled test concentrations and powdered ammonium sulfate (grounded in a mortar). 2 portions of the soil were mixed with quartz sand without the test substance and ammonium sulfate for blank control assays. The substrate proposed in OECD Guideline 216 (powdered plant matter) was not used because, as suggested in the guideline, there is not need to determine potential effect of the test substance (not being an agricultural chemical) on carbon transformation in soil. The potential variability in the carbon transformation process, which acts on the powdered plant matter to release ammonium, could therefore be avoided by direct application of a known quantity of a readily-available ammonium source. Therefore, in the modified test design, the plant meal amendment was omitted in all treatments, and not just the treatment associated with highest concentration of the test substance. - Test organisms (inoculum):
- soil
- Total exposure duration:
- 28 d
- Test temperature:
- 20.7-21.6 °C (2 times the scheduled temperature range was failed temporary with 22.9 °C and 23.3 °C. These filatures had no influence to the test.)
- Moisture:
- 40 ± 5% of the WHCmax
- Details on test conditions:
- TEST SYSTEM
- Testing facility: BASF SE, Experimental Toxicology and Ecology, 67056 Ludwigshafen, Germany
- Amount of soil: approx. 579 g
- No. of replicates per concentration: 2
- No. of replicates per control: 2
SOIL INCUBATION
- Method: bulk
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- History of site: The soil was not treated with crop protection products for more than 4 years. The sampling field was not cultivated for more than 4 years and no fertilizer application was performed during the last 4 years before sampling the soil.
- Vegetation cover: no data
- Treatments with pesticides or fertilizers: no
- Accidental contamination: no
- C/N ratio: The C:N ratio of the organic matter naturally present in the test soil was 9:1.1
- Depth of sampling: approx. 20 cm
- Soil classification system: Loamy sand according to DIN, sandy loam according to USDA
- pH (in water): 7.3 ± 0.1 (0.01M CaCl2)
- Initial nitrate concentration for nitrogen transformation test (mg nitrate/kg dry weight): The initial nitrate concentration for the nitrogen transformation test was determined using triplicate sample analyses from duplicate control test vessels at Day 0. The measured nitrate concentrations ranged from 217.89 to 230.19 mg/kg d.w. (5.6 % difference between lowest and highest values)
- Cation exchange capacity: 16.6 ± 2.8 meq/100 g
- Pretreatment of soil: 48 h before start of the test 9576.11 g of the soil were mixed with 846 mL deionized water.
- Storage (condition, duration): The soil was stored from 12 Oct 2012 to 15 Oct 2012 in a cooled storage room in a closed plastic sack at a temperature of 6.7 °C to 7.8 °C in the dark until usage.
- Initial microbial biomass as % of total organic C: 484.4 ± 11.8 mg carbon/kg Dw/h - Nominal and measured concentrations:
- 10, 20, 50, 125, 250, 500 and 1000 mg of the test substance per kg dry weight of soil (nominal)
- Reference substance (positive control):
- no
- Duration:
- 28 d
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: ammonium reduction
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: ammonium reduction
- Details on results:
- - Effect concentrations exceeding solubility of substance in test medium: no
- no effects on nitrate formation were detected but nor EC-values were derived as only ammonia reduction results were used for statistical ECx avaluation. - Validity criteria fulfilled:
- yes
- Conclusions:
- Nitrification in soil is not inhibited by the test substance neither be measuring the ammonia decrease nor by the formation of nitrate as endpoints.
Reference
- The basis for the reported effect endpoint was chosen as ammonium disappearance, and not nitrate formation, because the test substance has been shown to react directly with nitrite (a transient intermediate in the transformation of ammonium to nitrate). The biological transformation of ammonium to nitrate proceeds through nitrite as an intermediate. The study did not include analyses of, or information relating directly to, formation of nitrite. However, if inhibition of nitrite formation were to have occurred, this would be reflected in the results obtained for nitrate formation as a function of applied test substance and time.
- The standard test design of OECD Guideline 216 involves the catabolism of soil organic matter and a plant matter amendment, which is presumed to result in release of ammonium, which is subsequently oxidized to nitrate. It is well-known that primary aromatic amines, such as the test substance, can react directly with nitrite to form a stable diazonium compound. It is also well-known that the biological transformation of ammonium to nitrate (i.e., nitrification in soil) occurs via a transient nitrite intermediate. Where the presence of the reactive aromatic amine test substance is concurrent with presence of active biological nitrification, a reduced yield of nitrite (and thus nitrate) could be observed which is not directly caused by inhibition or toxicity to the nitrifying microorganisms. In other words, even where the test substance may not have a direct or indirect effect on conversion of ammonium to nitrate (nitrification), such an effect could be inferred if reduced yield of nitrate were observed as a result of unquantified reaction of the transient nitrite intermediate with the test substance.
Description of key information
Nitrifying bacteria are not inhibited by the test substance.
Key value for chemical safety assessment
- Short-term EC50 for soil microorganisms:
- 1 000 mg/kg soil dw
- Long-term EC10 or NOEC for soil microorganisms:
- 1 000 mg/kg soil dw
Additional information
Inhibition of nitrogen cycle in soils was investigates in a GLP test according to OECD guideline 216 (BASF, 2013). After 20 days the EC10 and EC50 was estimated to be >1000 mg/kg soil (d.w.), related to the decrease of ammonia. No adverse effect was observed up to the highest tested concentration. Regarding the nitrate formation, no effects were detected as well.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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