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EC number: 613-953-8 | CAS number: 66603-10-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu./o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Start of experiment: Apr. 28, 1980
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38 412; test procedure with water organisms (group L) general notes on the design, performance and evaluation of biological test procedures (L1) and determination of the effect of constituents of water on fishes - fish test (L15).
- Version / remarks:
- Draft, January 1979.
- Deviations:
- not specified
- Principles of method if other than guideline:
- German standard methods for the examination of water, waste water and sludge; bio-assays (group L); determination of the effect of substances in water on fish, fish-test (L15)
- GLP compliance:
- no
- Remarks:
- Test performed prior to the implementation of GLP
Test material
- Reference substance name:
- Cyclohexylhydroxydiazene 1-oxide, potassium salt
- EC Number:
- 613-953-8
- Cas Number:
- 66603-10-9
- Molecular formula:
- C6H11KN2O2
- IUPAC Name:
- Cyclohexylhydroxydiazene 1-oxide, potassium salt
- Test material form:
- liquid
- Details on test material:
- Xyligen 30F is a 30% aqueous solution of K-HDO.
Constituent 1
- Specific details on test material used for the study:
- N-cyclohexyldiazeniumdioxy potassium
30% technical active substance, 70% water
Purity: technical
Solubility in water: readily soluble
Lot/Batch Nr.: Test substance Nr.: 79/571
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Leuciscus idus
- Details on test organisms:
- TEST ORGANISM
- Common name: golden orfe
- Strain: Leuciscus idus L.
- Source: P. Eggers, Hohenwestedt, Germany
- Length at study initiation: Body length: 6.4 cm
- Weight at study initiation: Body weight: 1.9 g
ACCLIMATION
- Acclimation period: 3 days
- Type and amount of food during acclimation: "Holstenstolz A complete trout feed" type 34 Wilhelm Stroeh Jun. KG, Hobbersdorf-Luebeck, FRG; ad libitum
- Health during acclimation (any mortality observed): No mortality observed
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No
Test conditions
- Hardness:
- See table below
- Test temperature:
- 20°C ± 1°C
- pH:
- 8 ± 0.1
- Dissolved oxygen:
- > 8 mg/L
- Salinity:
- The following concentrations of salts were added to the completely demineralized water:
344 mg/L CaSO4 • 2H2O
124 mg/L MgSO4 • 7H2O
70 mg/L NaHCO3
3 mg/l KCL - Nominal and measured concentrations:
- No measurement of the test substance. However, KHDO is hydrolytically stable at pH 7 and 9. Furthermore, in all other aquatic tests the nominal concentrations were confirmed within analytical measurements.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 10 litres test water in glass aquaria (30 cm x 22 cm x 24 cm) (for 10 fish)
- Aeration: Continuous aeration with oil-free air
- Renewal rate of test solution (frequency/flow rate): No renewal of test solution
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Test water: prepared from completely demineralized water; pre-purification over an active charcoal filter, demineralization over an "Aquadem" type 42 DF cartridge for complete demineralization supplied by Wassertechnische Anlagen (WTA), Cologne, FRG
The following concentrations of salts were added:
344 mg/L CaSO4 • 2H2O
124 mg/L MgSO4 • 7H2O
70 mg/L NaHCO3
3 mg/l KCL
- Culture medium different from test medium: yes
OTHER TEST CONDITIONS
- Adjustment of pH: no details given in the study report
- Photoperiod: no details given in the study report
- Light intensity: no details given in the study report
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
Determination of the mortality after: 4h, 24h, 48h, 72h, 96 h
Determination or calculation of the median Lethal concentration (LC50), the LC5 and the LC95 by probit analysis after: 4h, 24h, 48h, 96 h
Signs after 4h and 48h
TEST CONCENTRATIONS
- Test concentrations: 46.4, 68.1, 100, 147, 215, 316, 464, 681 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Chloroacetamide
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 4 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 680 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other:
- Remarks:
- tumbling
- Key result
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 680 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other:
- Remarks:
- tumbling
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 340 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other:
- Remarks:
- tumbling
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 171 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other:
- Remarks:
- tumbling
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
- - Behavioural abnormalities: tumbling
- Mortality of control: Chloroacetamide positive control: LC50 about 27 mg/L (at 48 h); this result corresponds to normal sensitivity.
- Results with reference substance (positive control):
- Chloroacetamide positive control: LC50 about 27 mg/L (at 48 h)
This result corresponds to normal sensitivity.
Results are valid. - Reported statistics and error estimates:
- See table below
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Mortality data:
Number
Test-Substance
Concentration (nominal) [mg/L]24 h
48 h
72 h
96 h
0
0
0
0
0
46.4
0
0
0
0
68.1
0
0
0
0
100
0
0
0
0
147
0
2
2
2
215
0
1
5
9
316
0
3
9
10
464
3
10
10
10
681
2
10
10
10
Temperature [°C]
20
pH
7.7-7.9
Oxygen [mg/l]
7.8-8.3
Results of probit analysis:
95 % confidence limits
Concentration
lower
upper
LC50
171.0
147.5
198.6
LC5
128.0
80.6
148.2
LC95
228.5
197.3
364.2
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Golden orfe test: LC50 (96h): 171 mg/L
- Executive summary:
The acute toxicity test performed with 30 % K-HDO technical on Leuciscus idus L. (Golden orfe) produced the following results:
LC 50 after:
4 h
greater than
680 mg/l (10 % level of significance)
24 h
about
680 mg/l
48 h
about
340 mg/l (figure from intrapolation)
96 h
equal to
171 mg/l (slope factor = 1.19)
No effect level: 100mg/l
Highest concentration tested without mortality: 100 mg/l
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